CAJ | 학술논문

目的：观察电击死大鼠心脏超微结构及热休克蛋白70（heat shock protein 70，HSP70）、低氧诱导因子－1α（hypoxia inducible factor－1α，HIF－1α）的表达变化，探讨其是否能作为电击死法医学鉴定的依据。方法将72只SD大鼠随机分为电击死组、死后电击组和对照组。通过透射电镜观察电击死大鼠心肌超微结构的改变，并采用免疫组织化学技术观测心肌HSP70和HIF－1α的表达变化。结果电击大鼠心肌细胞肌原纤维断裂，线粒体嵴和膜溶解消失，Z线、M线排列紊乱。 HSP70和HIF－1α在电击死组呈强阳性表达，与死后电击组、对照组比较差异有统计学意义（P<0．05）。结论 HSP70和HIF－1α在电击死组表达明显上调，有望作为电击死的诊断参考指标。
목적：관찰전격사대서심장초미결구급열휴극단백70（heat shock protein 70，HSP70）、저양유도인자－1α（hypoxia inducible factor－1α，HIF－1α）적표체변화，탐토기시부능작위전격사법의학감정적의거。방법장72지SD대서수궤분위전격사조、사후전격조화대조조。통과투사전경관찰전격사대서심기초미결구적개변，병채용면역조직화학기술관측심기HSP70화HIF－1α적표체변화。결과전격대서심기세포기원섬유단렬，선립체척화막용해소실，Z선、M선배렬문란。 HSP70화HIF－1α재전격사조정강양성표체，여사후전격조、대조조비교차이유통계학의의（P<0．05）。결론 HSP70화HIF－1α재전격사조표체명현상조，유망작위전격사적진단삼고지표。
Objective T o observe cardiac ultrastructure and the expression of heat shock protein 70 (H SP70) and hypoxia inducible factor-1α (H IF-1α) in electric shock death rats and to explore the application of these indexes as the basis of m edical identification in electric shock death. Methods Seventy-tw o SD rats w ere random ly divided into electric shock death group, postm ortem electric shock group and the control group. T he changes of m yocardial ultrastructure w ere observed by transm ission electron m icro-scope, and the expressions of m yocardial H SP70 and H IF-1α w ere observed by im m unohistochem ical technology. Results M yocardial m yofibril fracture, m itochondrial cristae and m em brane dissolution, and disordered arrangem ent of Z lines and M lines w ere observed in electric shock rats. H SP70 and H IF-1αw ere strong positive expressions in the electric shock death group, significantly com pared w ith the con-trol and postm ortem electric shock groups (P<0.05). Conclusion T he expressions of H SP70 and H IF-1αw ere obviously increased in electric shock death group, w hich m ay be used as the diagnostic indicator of electric shock death.