CAJ | 학술논문

利用猪霍乱沙门氏菌lacZ基因设计特异性引物，通过优化各种反应条件，建立了检测猪霍乱沙门氏菌的环介导等温扩增（LAMP）快速检测方法。此方法特异性好，灵敏度高，将细菌DNA稀释到10-8仍可检出，是PCR方法的1000倍。本研究建立的LAMP方法操作简便、灵敏度高、特异性强，为临床检测猪霍乱沙门氏菌和预防人猪霍乱沙门氏菌病提供了技术保障。
이용저곽란사문씨균lacZ기인설계특이성인물，통과우화각충반응조건，건립료검측저곽란사문씨균적배개도등온확증（LAMP）쾌속검측방법。차방법특이성호，령민도고，장세균DNA희석도10-8잉가검출，시PCR방법적1000배。본연구건립적LAMP방법조작간편、령민도고、특이성강，위림상검측저곽란사문씨균화예방인저곽란사문씨균병제공료기술보장。
Based on the specific primers designed according to the lacZ gene of Salmonella choleraesuis a loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of Salmonella choleraesuis. The result could be observed both with agarose gel electrophoresis and naked eyes. The LAMP assay was of high sensitivity and specificity,and could detect as low as 10-8 dilutions. The study provided a very simple and efficient detection method for the clinical diagnosis of Salmonella choleraesuis in food,suitable for rapid detection in laboratories under general conditions.