中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2015年
8期
79-82,83
,共5页
张代宝%闫若潜%刘炜%贾松涛
張代寶%閆若潛%劉煒%賈鬆濤
장대보%염약잠%류위%가송도
鸡基因工程复合抗病毒制剂%冻干%保护剂%保存期
鷄基因工程複閤抗病毒製劑%凍榦%保護劑%保存期
계기인공정복합항병독제제%동간%보호제%보존기
chicken genetic engineering composite antiviral preparation%lyophilization%cryoprotectant%preserve period
采用不同高压灭菌条件处理的不同比例的脱脂奶粉、蔗糖、海藻糖以及维生素C组成的保护剂配方对鸡基因工程复合抗病毒制剂进行冻干,冻干后通过外观检查、pH值检测、复水性检测及效价测定比较筛选出了理想的冻干保护剂,之后将普通水剂型和冻干型鸡基因工程复合抗病毒制剂分别置于4℃、-20℃、-70℃条件下进行保存期试验,结果表明采用脱脂奶粉+蔗糖、脱脂奶粉+蔗糖+海藻糖+维生素C两种配方对鸡基因工程复合抗病毒制剂的保护效果最好,冻干后效价仍为220;普通水剂和冻干型鸡基因工程复合抗病毒制剂在-70℃保存条件下保存360天内效价均未降低,普通水剂分别于90天、180天后效价开始明显下降,而冻干型鸡基因工程复合抗病毒制剂的效价均无明显降低,稳定性良好。本研究初步筛选出了鸡基因工程抗病毒制剂的冻干配方,显著延长了其保存期,为鸡基因工程抗病毒制剂规模化生产和推广应用奠定了基础。
採用不同高壓滅菌條件處理的不同比例的脫脂奶粉、蔗糖、海藻糖以及維生素C組成的保護劑配方對鷄基因工程複閤抗病毒製劑進行凍榦,凍榦後通過外觀檢查、pH值檢測、複水性檢測及效價測定比較篩選齣瞭理想的凍榦保護劑,之後將普通水劑型和凍榦型鷄基因工程複閤抗病毒製劑分彆置于4℃、-20℃、-70℃條件下進行保存期試驗,結果錶明採用脫脂奶粉+蔗糖、脫脂奶粉+蔗糖+海藻糖+維生素C兩種配方對鷄基因工程複閤抗病毒製劑的保護效果最好,凍榦後效價仍為220;普通水劑和凍榦型鷄基因工程複閤抗病毒製劑在-70℃保存條件下保存360天內效價均未降低,普通水劑分彆于90天、180天後效價開始明顯下降,而凍榦型鷄基因工程複閤抗病毒製劑的效價均無明顯降低,穩定性良好。本研究初步篩選齣瞭鷄基因工程抗病毒製劑的凍榦配方,顯著延長瞭其保存期,為鷄基因工程抗病毒製劑規模化生產和推廣應用奠定瞭基礎。
채용불동고압멸균조건처리적불동비례적탈지내분、자당、해조당이급유생소C조성적보호제배방대계기인공정복합항병독제제진행동간,동간후통과외관검사、pH치검측、복수성검측급효개측정비교사선출료이상적동간보호제,지후장보통수제형화동간형계기인공정복합항병독제제분별치우4℃、-20℃、-70℃조건하진행보존기시험,결과표명채용탈지내분+자당、탈지내분+자당+해조당+유생소C량충배방대계기인공정복합항병독제제적보호효과최호,동간후효개잉위220;보통수제화동간형계기인공정복합항병독제제재-70℃보존조건하보존360천내효개균미강저,보통수제분별우90천、180천후효개개시명현하강,이동간형계기인공정복합항병독제제적효개균무명현강저,은정성량호。본연구초보사선출료계기인공정항병독제제적동간배방,현저연장료기보존기,위계기인공정항병독제제규모화생산화추엄응용전정료기출。
The chicken genetic engineering composite antiviral preparation was freeze-dried with different cryopro-tectant prescriptions made up with skim milk,sucrose,trehalose and Vc by means of different autoclaved steriliza-tion. The ideal cryoprotectant was selected through appearance examination,pH measurement,rehydration test and potency test. The aqueous chicken genetic engineering composite antiviral preparation and lyophilized chicken genetic engineering composite antiviral preparation were placed under 4℃,-20℃,-70℃for preservation period test. The result suggested that the two cryoprotectant prescriptions(skim milk+sucrose and skim milk+sucrose+trehalose+Vc)could best protect chicken genetic engineering composite antiviral preparation with the antiviral titer of 220. The preservation period test showed that the aqueous chicken genetic engineering composite antiviral preparation and the ly-ophilized chicken genetic engineering composite antiviral preparation under-70℃could be preserved for one year with no decline of the titer;while under 4℃and-20℃,the titers of the aqueous preparation started to decline respectively after 90d and 180d,and the titer of the lyophilized preparation did not decline under-20℃and decreased a little under 4℃after 180d. The result indicated that the selected cryoprotectants could remarkably extend the preservation period of the chicken genetic engineering composite antiviral preparation. The study selected the cryoprotectant of chicken genetic engineering composite antiviral preparation,successfully prepared lyophilized chicken genetic engineering composite antiviral preparation with good appearance. It also lay the foundation for the large-scale production of chicken genetic engineering composite antiviral preparation and made some contribution to clinic promotion and application.