中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2015年
24期
19-20
,共2页
肝硬化%腹水%内毒素%肿瘤坏死因子-α%白细胞介素-6
肝硬化%腹水%內毒素%腫瘤壞死因子-α%白細胞介素-6
간경화%복수%내독소%종류배사인자-α%백세포개소-6
Hepatic sclerosis%Ascites%Endotoxin%Tumor necrosis factor-α%Interleukin-6
目的 探讨肝硬化自发性细菌性腹膜炎(SBP)患者和肝硬化伴无菌性腹水(SA)患者体内前炎症细胞因子肿瘤坏死因子-α(TNF-α) 、白细胞介素-6(IL-6)的水平及改变的临床意义.方法 采用双抗体夹心酶联免疫吸附法(ELISA)检测 188例肝炎肝硬化腹水患者[其中肝硬化伴有自发性细菌性腹膜炎(SBP组)78例, 肝硬化伴有无菌性腹水(SA组)110例]腹水、血液标本中TNF-α、IL-6水平, 同时应用鲎试剂三肽显色基质偶氮法检测标本内毒素(LPS)水平.同期20例健康体检者作为健康对照组, 并对三组LPS、TNF-α、IL-6水平进行比较.结果 SBP组、SA组血浆LPS、TNF-α、IL-6浓度均显著高于健康对照组(P<0.001); SBP组腹水LPS、TNF-α、IL-6浓度均显著高于SA组(P<0.001);肝硬化腹水患者血浆内毒素与TNF-α、IL-6水平呈正相关(P<0.05).结论 肝炎肝硬化腹水患者存在内毒素血症, 肝炎肝硬化腹水患者TNF-α、IL-6水平升高与体内内毒素水平升高有关.
目的 探討肝硬化自髮性細菌性腹膜炎(SBP)患者和肝硬化伴無菌性腹水(SA)患者體內前炎癥細胞因子腫瘤壞死因子-α(TNF-α) 、白細胞介素-6(IL-6)的水平及改變的臨床意義.方法 採用雙抗體夾心酶聯免疫吸附法(ELISA)檢測 188例肝炎肝硬化腹水患者[其中肝硬化伴有自髮性細菌性腹膜炎(SBP組)78例, 肝硬化伴有無菌性腹水(SA組)110例]腹水、血液標本中TNF-α、IL-6水平, 同時應用鱟試劑三肽顯色基質偶氮法檢測標本內毒素(LPS)水平.同期20例健康體檢者作為健康對照組, 併對三組LPS、TNF-α、IL-6水平進行比較.結果 SBP組、SA組血漿LPS、TNF-α、IL-6濃度均顯著高于健康對照組(P<0.001); SBP組腹水LPS、TNF-α、IL-6濃度均顯著高于SA組(P<0.001);肝硬化腹水患者血漿內毒素與TNF-α、IL-6水平呈正相關(P<0.05).結論 肝炎肝硬化腹水患者存在內毒素血癥, 肝炎肝硬化腹水患者TNF-α、IL-6水平升高與體內內毒素水平升高有關.
목적 탐토간경화자발성세균성복막염(SBP)환자화간경화반무균성복수(SA)환자체내전염증세포인자종류배사인자-α(TNF-α) 、백세포개소-6(IL-6)적수평급개변적림상의의.방법 채용쌍항체협심매련면역흡부법(ELISA)검측 188례간염간경화복수환자[기중간경화반유자발성세균성복막염(SBP조)78례, 간경화반유무균성복수(SA조)110례]복수、혈액표본중TNF-α、IL-6수평, 동시응용후시제삼태현색기질우담법검측표본내독소(LPS)수평.동기20례건강체검자작위건강대조조, 병대삼조LPS、TNF-α、IL-6수평진행비교.결과 SBP조、SA조혈장LPS、TNF-α、IL-6농도균현저고우건강대조조(P<0.001); SBP조복수LPS、TNF-α、IL-6농도균현저고우SA조(P<0.001);간경화복수환자혈장내독소여TNF-α、IL-6수평정정상관(P<0.05).결론 간염간경화복수환자존재내독소혈증, 간염간경화복수환자TNF-α、IL-6수평승고여체내내독소수평승고유관.
Objective To investigate clinical significance of level changes of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in hepatic sclerosis complicated with spontaneous bacterial peritonitis (SBP) patients and hepatic sclerosis complicated with sterile ascites (SA) patients.Methods Double antibody enzyme-linked immune sorbent assays (ELISA) was applied to detect levels of ascites, TNF-α and IL-6 in blood samples of 188 hepatic sclerosis patients. Among them there were 78 cases of hepatic sclerosis complicated with spontaneous bacterial peritonitis (SBP group) and 110 cases of hepatic sclerosis complicated with sterile ascites (SA group). Tachypleus amebocyte lysate tripeptide chromogenic substrate azo method was applied to detect endotoxin (LPS) levels. There were another healthy people at the same period as health control group. Comparisons were made on LPS, TNF-α, and IL-6 levels in the three groups. Results SBP group and SA group both had obviously higher levels of plasma LPS, TNF-α and IL-6 than the health control group (P<0.001). SBP group had much higher levels of ascites LPS, TNF-α and IL-6 than SA group (P<0.001). Plasma LPS in hepatic sclerosis ascites patients had positive correlation with TNF-α and IL-6 (P<0.05).Conclusion Endotoxemia exists in hepatitis cirrhotic ascites patients. Increased TNF-α and IL-6 are related with increased endotoxin in hepatitis cirrhotic ascites patients.
