中国医药生物技术
中國醫藥生物技術
중국의약생물기술
CHINESE MEDICINAL BIOTECHNOLOGY
2015年
4期
356-360
,共5页
僵蚕%白僵菌属%草酸铵%固体发酵
僵蠶%白僵菌屬%草痠銨%固體髮酵
강잠%백강균속%초산안%고체발효
BOMBYX BATRYTICATUS%Beauveria%Ammonium oxalate%Solid fermentation
目的 改进中药僵蛹的发酵方法,以提高其活性成分草酸铵的含量.方法 以蚕蛹为发酵基质,通过接入白僵菌液体菌种制备形成僵蛹.以草酸铵的含量为主要考察指标,结合基质转化率,研究不同接种量对发酵工艺的影响,并与生药僵蚕中草酸铵的含量进行比较.结果 与空白对照比较,随着白僵菌接种量的加大,僵蛹中草酸铵的含量增加,其中以接种量为 20%时含量达最大,为 7.72%,在 5% ~ 25%接种量范围内,僵蛹中草酸铵的含量均高于四个不同产地生僵蚕的含量.结论 该固体发酵法简单、易行、可控,不易污染杂菌,能有效地提高活性成分草酸铵的含量,制备形成的僵蛹某种程度上可作为僵蚕的代用品.
目的 改進中藥僵蛹的髮酵方法,以提高其活性成分草痠銨的含量.方法 以蠶蛹為髮酵基質,通過接入白僵菌液體菌種製備形成僵蛹.以草痠銨的含量為主要攷察指標,結閤基質轉化率,研究不同接種量對髮酵工藝的影響,併與生藥僵蠶中草痠銨的含量進行比較.結果 與空白對照比較,隨著白僵菌接種量的加大,僵蛹中草痠銨的含量增加,其中以接種量為 20%時含量達最大,為 7.72%,在 5% ~ 25%接種量範圍內,僵蛹中草痠銨的含量均高于四箇不同產地生僵蠶的含量.結論 該固體髮酵法簡單、易行、可控,不易汙染雜菌,能有效地提高活性成分草痠銨的含量,製備形成的僵蛹某種程度上可作為僵蠶的代用品.
목적 개진중약강용적발효방법,이제고기활성성분초산안적함량.방법 이잠용위발효기질,통과접입백강균액체균충제비형성강용.이초산안적함량위주요고찰지표,결합기질전화솔,연구불동접충량대발효공예적영향,병여생약강잠중초산안적함량진행비교.결과 여공백대조비교,수착백강균접충량적가대,강용중초산안적함량증가,기중이접충량위 20%시함량체최대,위 7.72%,재 5% ~ 25%접충량범위내,강용중초산안적함량균고우사개불동산지생강잠적함량.결론 해고체발효법간단、역행、가공,불역오염잡균,능유효지제고활성성분초산안적함량,제비형성적강용모충정도상가작위강잠적대용품.
Objective To increase the content of active constituent by improving the fermentative preparation method of Pupa Bombycis Batryticatus.Methods It was prepared with Bombyx mori L. as the substrate inoculated with the fungi,Beauveria bassiana(Bals.) Vuillant. Effects of different inoculation amounts on the fermentation technology were checked, with the content of ammonium oxalate combined with matrix conversion rate as the main indexes. Furthermore, the contents of fermentation products were compared with those of the crude drug, Bombyx Batryticatus.Results Compared with the control group, the content of ammonium oxalate in Pupa Bombycis Batryticatus was raised with the increase of inoculation amount. When the inoculation amount was 20%, the content reached the highest (7.72%). Within the range from 5% to 25% inoculation amount, the contents in fermentation products were all higher than that in crude drugs from four different producing areas.Conclusion The preparation technology is a simple and controllable method. It isn't easily contaminated and can increase the content of active constituent effectively. The product, Pupa Bombycis Batryticatus, prepared by this method will be the alternative of Bombyx Batryticatus to some degree.
