CAJ | 학술논문

目的研究链霉菌 CPCC 203702中的抗结核分枝杆菌活性次级代谢产物.方法发酵液离心后,上清液经乙酸乙酯萃取、正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱和半制备 HPLC等方法进行以抗耻垢分枝杆菌活性为导向的活性化合物分离纯化,通过质谱和核磁共振波谱技术并结合文献数据进行化合物结构鉴定;分别采用平板纸片法和阿尔玛蓝法检测目的化合物的抗耻垢分枝杆菌以及抗结核分枝杆菌活性.结果从链霉菌 CPCC 203702发酵液中分离到 1个具有显著的抗结核分枝杆菌活性的伯尔尼霉素类化合物伯尔尼霉素 C,其抗结核分枝杆菌 MIC为 2μg/ml;完成其核磁数据的完整归属.结论首次报道了伯尔尼霉素类抗生素具有显著的抗结核分枝杆菌活性,丰富了硫肽类抗生素的活性研究,也为抗结核药物的发现提供一个新的视角.
목적 연구련매균 CPCC 203702중적항결핵분지간균활성차급대사산물.방법 발효액리심후,상청액경을산을지췌취、정상규효주색보、Sephadex LH-20응효주색보화반제비 HPLC등방법진행이항치구분지간균활성위도향적활성화합물분리순화,통과질보화핵자공진파보기술병결합문헌수거진행화합물결구감정;분별채용평판지편법화아이마람법검측목적화합물적항치구분지간균이급항결핵분지간균활성.결과 종련매균 CPCC 203702발효액중분리도 1개구유현저적항결핵분지간균활성적백이니매소류화합물백이니매소 C,기항결핵분지간균 MIC위 2μg/ml;완성기핵자수거적완정귀속.결론 수차보도료백이니매소류항생소구유현저적항결핵분지간균활성,봉부료류태류항생소적활성연구,야위항결핵약물적발현제공일개신적시각.
Objective To investigate isolation and identification of anti-Mycobacterium tuberculosis secondary metabolites from Streptomyces sp.CPCC 203702. Methods The fermentation culture was separated into the supernatant and mycelia by using low-speed centrifugation. The supernatant was extracted with EtOAc, and the EtOAc extract was isolated and purified by combined use of silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The anti-Mycobacterium tuberculosis activity was tested by MABA method. The structure of isolated pure compound was elucidated by extensive spectroscopic analyses of NMR and MS, as well as by comparing the reported NMR values in the literatures. Results Berninamycin C was isolated from the supernatant of Streptomyces sp.CPCC 203702. It showed significant anti-Mycobacterium tuberculosis activity with MIC value of 2μg/ml. Conclusion Berninamycin C is obtained from the supernatant of Streptomyces sp.CPCC 203702 and its 1H- and 13C-NMR data are fully assigned for the first time. Berninamycin C shows remarkable anti-Mycobacterium tuberculosis activity which is never reported before.