合肥工业大学学报（自然科学版）
閤肥工業大學學報（自然科學版）
합비공업대학학보（자연과학판）
JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE)
2015年
7期
992-996
,共5页
赵翔%方丽%彭书传%吴克
趙翔%方麗%彭書傳%吳剋
조상%방려%팽서전%오극
多氯联苯降解菌%枯草杆菌W F1%联苯双加氧酶%活性酶%酶学性质
多氯聯苯降解菌%枯草桿菌W F1%聯苯雙加氧酶%活性酶%酶學性質
다록련분강해균%고초간균W F1%련분쌍가양매%활성매%매학성질
polychlorinated biphenyls(PCBs)-degrading strain%Bacillus subtilis WF1%biphenyl dioxy-genase%active enzyme%enzymatic characteristics
文章研究了多氯联苯降解菌所产联苯双加氧酶的酶学性质，结果表明联苯双加氧酶在多氯联苯降解的过程中起到了重要作用。此降解酶为胞内酶，最适温度为35℃，最适pH值为7.5，Na＋促进反应，K＋基本没有影响，M g2＋、Ca2＋、Fe2＋、Cu2＋均有不同程度的抑制作用。以米氏方程为“模型”，确定了动力学方程及其重要的动力学特征参数。
文章研究瞭多氯聯苯降解菌所產聯苯雙加氧酶的酶學性質，結果錶明聯苯雙加氧酶在多氯聯苯降解的過程中起到瞭重要作用。此降解酶為胞內酶，最適溫度為35℃，最適pH值為7.5，Na＋促進反應，K＋基本沒有影響，M g2＋、Ca2＋、Fe2＋、Cu2＋均有不同程度的抑製作用。以米氏方程為“模型”，確定瞭動力學方程及其重要的動力學特徵參數。
문장연구료다록련분강해균소산련분쌍가양매적매학성질，결과표명련분쌍가양매재다록련분강해적과정중기도료중요작용。차강해매위포내매，최괄온도위35℃，최괄pH치위7.5，Na＋촉진반응，K＋기본몰유영향，M g2＋、Ca2＋、Fe2＋、Cu2＋균유불동정도적억제작용。이미씨방정위“모형”，학정료동역학방정급기중요적동역학특정삼수。
The enzymatic characteristics of the biphenyl dioxygenase from a polychlorinated biphenyls (PCBs)-degrading strain was investigated .The result showed that biphenyl dioxygenase played an im-portant role in the degradation process of PCBs .It belonged to endoenzyme ,and the most suitable pH value and temperature for this enzyme were at 7.5 and 35 ℃ ,respectively .The natrium ion could ac-celerate the activity of the enzyme ,the kalium ion had no effect on it ,and the magnesium ion ,calcium ion ,ferric ion and copper ion could suppress the activity at a certain degree .The Michaelis-Menten e-quation w as adopted to simulate the enzymatic process ,and the kinetic equation and key kinetic pa-rameters w ere achieved .
