CAJ | 학술논문

目的观察卡介苗多糖核酸(BCG-PSN)对哮喘大鼠支气管肺泡灌洗液(BALF)、外周血和淋巴液中CD4+IL-17+T细胞(CD4+IL-17+T)与CD4+Foxp3+调节性T细胞(CD4+Foxp3+ Treg)百分比及IL-10、IL-17水平的影响及其可能机制.方法 SD大鼠60只随机分为对照组(n=15)、哮喘组(n=15)、BCG-PSN干预对照组(n=15)和BCG-PSN干预哮喘组(n=15).哮喘组和BCG-PSN干预哮喘组使用卵白蛋白建立大鼠哮喘模型,对照组和BCG-PSN干预对照组以等量生理盐水代替卵白蛋白,BCG-PSN干预哮喘组和BCG-PSN干预对照组均使用BCG-PSN进行干预.收集各组大鼠BALF、外周血和淋巴液,采用流式细胞术检测CD4+IL-17+T和CD4+Foxp3+ Treg百分比,酶联免疫吸附法(ELISA)检测IL-10和IL-17水平.结果各组大鼠淋巴液中CD4+Foxp3+ Treg百分比、IL-10水平较BALF和外周血均明显升高(均P＜0.01),CD4+IL-17+T百分比、IL-17水平较BALF和外周血均明显下降(均P＜0.01).哮喘组大鼠BALF、淋巴液和外周血CD4+Foxp3+ Treg百分比、IL-10水平较其余3组均显著降低,而CD4+IL-17+T百分比、IL-17水平较其余3组均显著升高(均P＜0.01).BCG-PSN干预哮喘组大鼠BALF、淋巴液和外周血CD4+Foxp3+ Treg百分比和IL-10水平较哮喘组显著升高(均P＜0.01),与对照组和BCG-PSN干预对照组比较差异无统计学意义(均P＞0.05).BCG-PSN干预哮喘组大鼠BALF、淋巴液和外周血CD4+IL-17+T细胞百分比、IL-17水平较哮喘组均显著下降(均P＜0.01),与对照组和BCG-PSN干预对照组比较差异无统计学意义(P＞0.05).结论 BCG-PSN可能通过调节哮喘大鼠外周血和淋巴液中CD4+Foxp3+ Treg和CD4+IL-17+T的百分比及相关细胞因子水平,改善机体免疫功能,从而减轻哮喘的炎性反应.
목적 관찰잡개묘다당핵산(BCG-PSN)대효천대서지기관폐포관세액(BALF)、외주혈화림파액중CD4+IL-17+T세포(CD4+IL-17+T)여CD4+Foxp3+조절성T세포(CD4+Foxp3+ Treg)백분비급IL-10、IL-17수평적영향급기가능궤제.방법 SD대서60지수궤분위대조조(n=15)、효천조(n=15)、BCG-PSN간예대조조(n=15)화BCG-PSN간예효천조(n=15).효천조화BCG-PSN간예효천조사용란백단백건립대서효천모형,대조조화BCG-PSN간예대조조이등량생리염수대체란백단백,BCG-PSN간예효천조화BCG-PSN간예대조조균사용BCG-PSN진행간예.수집각조대서BALF、외주혈화림파액,채용류식세포술검측CD4+IL-17+T화CD4+Foxp3+ Treg백분비,매련면역흡부법(ELISA)검측IL-10화IL-17수평.결과 각조대서림파액중CD4+Foxp3+ Treg백분비、IL-10수평교BALF화외주혈균명현승고(균P＜0.01),CD4+IL-17+T백분비、IL-17수평교BALF화외주혈균명현하강(균P＜0.01).효천조대서BALF、림파액화외주혈CD4+Foxp3+ Treg백분비、IL-10수평교기여3조균현저강저,이CD4+IL-17+T백분비、IL-17수평교기여3조균현저승고(균P＜0.01).BCG-PSN간예효천조대서BALF、림파액화외주혈CD4+Foxp3+ Treg백분비화IL-10수평교효천조현저승고(균P＜0.01),여대조조화BCG-PSN간예대조조비교차이무통계학의의(균P＞0.05).BCG-PSN간예효천조대서BALF、림파액화외주혈CD4+IL-17+T세포백분비、IL-17수평교효천조균현저하강(균P＜0.01),여대조조화BCG-PSN간예대조조비교차이무통계학의의(P＞0.05).결론 BCG-PSN가능통과조절효천대서외주혈화림파액중CD4+Foxp3+ Treg화CD4+IL-17+T적백분비급상관세포인자수평,개선궤체면역공능,종이감경효천적염성반응.
Objective To investigate the effect of BCG-polysaccharide nucleic acid (BCG-PSN) on the levels of CD4+IL-17+ T cells and CD4+Foxp3+ regulatory T cells(Treg),interleukin-10 (IL-10) and IL-17 in bronchoalveolar lavage fluid (BALF),peripheral blood and lymph fluids from asthmatic rats and the possible mechanism.Methods Sixty SD rats were randomly divided into control group (n=15),asthma group (n=15),BCG-PSN control group (n=15) and BCG-PSN asthma group (n=15),respectively.The rats in the asthma group and the BCG-PSN asthma group were modeled to be of asthma by challenging with ovalbumin (OVA),while those in control group and BCG-PSN control group were sham-challenged with equal volume of normal saline.The BCG-PSN asthma group and the BCG-PSN control group were treated with BCG-PSN as an intervention.The rat BALF,peripheral blood and lymph fluids were collected from each group.Flow cytometry (FCM) was used to determine the percentage of CD4+IL-17+ T cells and CD4+Foxp3+ Treg.Enzyme-linked immunosorbent assay (ELISA) was used to determine the IL-10 and IL-17 levels.Results All groups showed significantly higher CD4+Foxp3+ Treg percentage and IL-10 level,lower CD4+ IL-17+ T cells percentage and IL-17 level in lymph fluid than those in blood and BALF (all P＜0.01).In asthma group,the percentage of CD4+Foxp3+ Treg,IL-10 were significantly lower,and CD4+IL-17+ T cells percentage and IL-17 level were higher compared with those in BALF,lymph fluid and peripheral blood in the other 3 groups (all P＜0.01).In BCG-PSN asthma group,there were higher CD4+Foxp3+ Treg percentage and IL-10 level,lower CD4+IL-17+ T cells percentage and IL-17 level in BALF,lymph fluid and peripheral blood compared with the asthma group (all P＜0.01),but these values were statistically comparable to those in the control group and BCG-PSN control group (all P＞0.05).Conclusion BCG-PSN may improve host immune response and thereby ameliorate the inflammation in asthma through regulating the percentage of CD4+Foxp3+ Treg and CD4+IL-17+ T cells,and the level of certain cytokines in lymph and peripheral blood of asthma rats.