中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2015年
3期
242-246
,共5页
刘天文%张杰民%刘志刚%刘晓程
劉天文%張傑民%劉誌剛%劉曉程
류천문%장걸민%류지강%류효정
左心辅助装置%心力衰竭%游离血红蛋白
左心輔助裝置%心力衰竭%遊離血紅蛋白
좌심보조장치%심력쇠갈%유리혈홍단백
Left ventricular auxiliary device%Heart failure%Free hemoglobin
目的 应用体外模拟循环实验装置,对磁液悬浮离心式心室辅助装置(以下简称血泵)进行评估.通过动物实验,验证血泵的性能及可靠性.对血泵持续优化、改进.方法 取新鲜羊血500 ml,3.8％枸橼酸钠1∶9抗凝.固定转速下,调节流出道管径,使流出道和流入道压力差值在100±2mmHg.取转泵前和转后1、2、3、4h血样标本,测量其游离血红蛋白(Free Hemoglobin,FHB)含量.应用非体外循环、不停跳方法,将血泵入口植入绵羊左心尖,出口与降主动脉吻合.记录手术前后血泵的参数及绵羊的相关生化、血液指标.结果 体外实验中,血泵运行平稳,温升≤2.6℃.测得国际标准溶血指数(Normalized Index of Hemolysis,NIH)为0.002～0.006 g/100 L.在体动物实验,血泵辅助流量60％,血浆中游离血红蛋白＜45 mg/L.结论 经改进的血泵,整体参数得到优化,满足心室辅助需求.在体实验技术成熟,可开展大样本临床前动物实验.
目的 應用體外模擬循環實驗裝置,對磁液懸浮離心式心室輔助裝置(以下簡稱血泵)進行評估.通過動物實驗,驗證血泵的性能及可靠性.對血泵持續優化、改進.方法 取新鮮羊血500 ml,3.8％枸櫞痠鈉1∶9抗凝.固定轉速下,調節流齣道管徑,使流齣道和流入道壓力差值在100±2mmHg.取轉泵前和轉後1、2、3、4h血樣標本,測量其遊離血紅蛋白(Free Hemoglobin,FHB)含量.應用非體外循環、不停跳方法,將血泵入口植入綿羊左心尖,齣口與降主動脈吻閤.記錄手術前後血泵的參數及綿羊的相關生化、血液指標.結果 體外實驗中,血泵運行平穩,溫升≤2.6℃.測得國際標準溶血指數(Normalized Index of Hemolysis,NIH)為0.002～0.006 g/100 L.在體動物實驗,血泵輔助流量60％,血漿中遊離血紅蛋白＜45 mg/L.結論 經改進的血泵,整體參數得到優化,滿足心室輔助需求.在體實驗技術成熟,可開展大樣本臨床前動物實驗.
목적 응용체외모의순배실험장치,대자액현부리심식심실보조장치(이하간칭혈빙)진행평고.통과동물실험,험증혈빙적성능급가고성.대혈빙지속우화、개진.방법 취신선양혈500 ml,3.8％구연산납1∶9항응.고정전속하,조절류출도관경,사류출도화류입도압력차치재100±2mmHg.취전빙전화전후1、2、3、4h혈양표본,측량기유리혈홍단백(Free Hemoglobin,FHB)함량.응용비체외순배、불정도방법,장혈빙입구식입면양좌심첨,출구여강주동맥문합.기록수술전후혈빙적삼수급면양적상관생화、혈액지표.결과 체외실험중,혈빙운행평은,온승≤2.6℃.측득국제표준용혈지수(Normalized Index of Hemolysis,NIH)위0.002～0.006 g/100 L.재체동물실험,혈빙보조류량60％,혈장중유리혈홍단백＜45 mg/L.결론 경개진적혈빙,정체삼수득도우화,만족심실보조수구.재체실험기술성숙,가개전대양본림상전동물실험.
Objective To evaluate the magnetic and hydrodynamic suspension-centrifugal ventricular auxiliary device (blood pump) by using stimulated circulating experimental device in vitro,and to verify the performance and reliability of the blood pump in animal experiment,for continual optimization and improvement of the device.Methods 500 ml fresh sheep blood was obtained,added with 3.8％ sodium citrate in a volume ratio of 1:9 for anticoagulation.Under the fixed rotation speed of the blood pump,the pipe diameter of the outflow tract was regulated to maintain the outflow and inflow tract pressure difference at 100± 2 mmHg.Before and after the rotation in the blood pump at 1,2,3 and 4 hours,the blood samples were obtained and the free hemoglobin (FHB) contents were measured.Using off-pump method,the blood pump inlet was implanted into the left cardiac apex of the sheep,and the outlet was sutured with the descending aorta.The blood pump parameters,and the biochemical parameters and blood parameters before and after surgery in the sheep were recorded.Results In the in vitro experiment,the blood pump operated steadily,the temperature rise ≤2.6 ℃,and the Normalized Index of Hemolysis (NIH) was 0.002 ～ 0.006 g/100L.In the in vivo animal experiment,the blood pump flow was 60％,and the plasma FHB＜45 mg/L.The seventh generation of the blood pump achieved great improvement.Conclusion In the in vitro experiment,the blood pump operated steadily,the temperature rise ≤2.6 ℃,and the Normalized Index of Hemolysis (NIH) was 0.002～0.006 g/100L.In the in vivo animal experiment,the blood pump flow was 60％,and the plasma FHB＜45 mg/L.The seventh generation of the blood pump achieved great improvement.
