环境科技
環境科技
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ENVIRONMENTAL SCIENCE AND TECHNOLOGY
2015年
4期
12-17,21
,共7页
代鹏飞%蔡天明%汪林%张炜铭%吕路%邵燕%赵雪梅%杜倩%杨洁%李丽芳%石家杰
代鵬飛%蔡天明%汪林%張煒銘%呂路%邵燕%趙雪梅%杜倩%楊潔%李麗芳%石傢傑
대붕비%채천명%왕림%장위명%려로%소연%조설매%두천%양길%리려방%석가걸
烟嘧磺隆%假单胞菌属%生物降解%降解途径
煙嘧磺隆%假單胞菌屬%生物降解%降解途徑
연밀광륭%가단포균속%생물강해%강해도경
Nicosulfuron%Pseudomonas sp.%Biodegradation%Metabolic pathway
从农药厂污水处理池的活性污泥中分离到1株能以烟嘧磺隆为唯一碳源生长的细菌，命名为YN-8。根据表型特征、生理生化特性及16S rRNA序列同源性分析，将该菌株初步鉴定为假单胞菌属(Pseudomonas sp.)。 YN-8能在以烟嘧磺隆为唯一碳源的基础盐液体培养基中生长，培养7 d，烟嘧磺隆的去除率达65.4%。该菌降解烟嘧磺隆的最适pH值为7.0，最佳接种量为5%，最适质量浓度为100 mg/L。当金属离子浓度为1 mmol/L时，Co2+和Ni2+抑制菌株对烟嘧磺隆的降解。通过液相色谱-质谱联用检测菌株降解烟嘧磺隆的代谢产物，初步推测烟嘧磺隆的降解途径为磺酰脲桥被打断，生成2-氨基-4,6-二甲氧基嘧啶和3-(二甲基烟酰胺)吡啶-2-基磺酰氨基甲酸。
從農藥廠汙水處理池的活性汙泥中分離到1株能以煙嘧磺隆為唯一碳源生長的細菌，命名為YN-8。根據錶型特徵、生理生化特性及16S rRNA序列同源性分析，將該菌株初步鑒定為假單胞菌屬(Pseudomonas sp.)。 YN-8能在以煙嘧磺隆為唯一碳源的基礎鹽液體培養基中生長，培養7 d，煙嘧磺隆的去除率達65.4%。該菌降解煙嘧磺隆的最適pH值為7.0，最佳接種量為5%，最適質量濃度為100 mg/L。噹金屬離子濃度為1 mmol/L時，Co2+和Ni2+抑製菌株對煙嘧磺隆的降解。通過液相色譜-質譜聯用檢測菌株降解煙嘧磺隆的代謝產物，初步推測煙嘧磺隆的降解途徑為磺酰脲橋被打斷，生成2-氨基-4,6-二甲氧基嘧啶和3-(二甲基煙酰胺)吡啶-2-基磺酰氨基甲痠。
종농약엄오수처리지적활성오니중분리도1주능이연밀광륭위유일탄원생장적세균，명명위YN-8。근거표형특정、생리생화특성급16S rRNA서렬동원성분석，장해균주초보감정위가단포균속(Pseudomonas sp.)。 YN-8능재이연밀광륭위유일탄원적기출염액체배양기중생장，배양7 d，연밀광륭적거제솔체65.4%。해균강해연밀광륭적최괄pH치위7.0，최가접충량위5%，최괄질량농도위100 mg/L。당금속리자농도위1 mmol/L시，Co2+화Ni2+억제균주대연밀광륭적강해。통과액상색보-질보련용검측균주강해연밀광륭적대사산물，초보추측연밀광륭적강해도경위광선뇨교피타단，생성2-안기-4,6-이갑양기밀정화3-(이갑기연선알)필정-2-기광선안기갑산。
A bacterial strain YN-8, capable o f utilizing nicosulfuron as sole carbon, was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer. This strain was identified as Pseudomonas sp. according to its morphological, physiological and biochemical analysis and the analysis of its 16S rRNA gene sequence. This strain could degrade nicosulfuron in its 100 mg/L liquor medium, with degradation efficiency reaching 65.4%in 7 days. The optimum pH, inoculation and concentration for nicosulfuron degradation by strain YN-8 were 7.0, 5% and 100 mg/L, respectively. In the tested metal ions, Co2+and Ni2+could inhibit the nicosulfuron degradation rate. According to the analysis of the metabolites detected by liquid chromatography-mass spectrometry, we proposed the following pathway of nicusulfuron biodegradation by strain: cleavage of the sulfonylurea bridge and generate 3-(dimethylcarbamoyl) pyridin-2-ylsulfonylcarbamic acid and 2-Amino-4, 6-dimethoxypyrimidine.
