中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
7期
577-579
,共3页
李吕力%韦俊杰%李晓峰%封浑%欧阳列锋%姜炳坚%张丽香%李燕华
李呂力%韋俊傑%李曉峰%封渾%歐暘列鋒%薑炳堅%張麗香%李燕華
리려력%위준걸%리효봉%봉혼%구양렬봉%강병견%장려향%리연화
丁苯酞%脑缺血再灌注%血管细胞黏附分子-1%细胞间黏附分子-1
丁苯酞%腦缺血再灌註%血管細胞黏附分子-1%細胞間黏附分子-1
정분태%뇌결혈재관주%혈관세포점부분자-1%세포간점부분자-1
Butylphthalide%Middle cerebral artery ischemia-reperfusion%Vascular cell adhesion molecule-1%Intercellular adhesion molecule-1
目的 探索丁苯酞对脑缺血再灌注大鼠的血管细胞黏附分子-1(VCAM-1)和细胞间黏附分子-1(ICAM-1)的影响.方法 用随机数表法将30只雄性SD大鼠分为3组:假手术组、模型组和丁苯酞预处理组,每组10只;采用大脑中动脉闭塞法建立大鼠脑缺血再灌注模型,采用免疫组化检测大脑的VCAM-1和ICAM-1表达,免疫酶联吸附法检测血清的VCAM-1和ICAM-1浓度.结果 模型组大脑和血清的VCAM-1和ICAM-1均高于假手术组(P＜0.05).与模型组比较,丁苯酞预处理组大脑和血清的ICAM-1差异无统计学意义(P＞0.05),而丁苯酞预处理组大脑的VCAM-1水平[(0.127±0.017) ng/ml]和血清的VCAM-1水平[(160.22±53.95) ng/ml]低于模型组[大脑:(0.164±0.025) ng/ml;血清:(270.89 ± 76.49)ng/ml,P＜0.05].结论 丁苯酞具有抑制脑缺血再灌注大鼠大脑和血清VCAM-1的作用.
目的 探索丁苯酞對腦缺血再灌註大鼠的血管細胞黏附分子-1(VCAM-1)和細胞間黏附分子-1(ICAM-1)的影響.方法 用隨機數錶法將30隻雄性SD大鼠分為3組:假手術組、模型組和丁苯酞預處理組,每組10隻;採用大腦中動脈閉塞法建立大鼠腦缺血再灌註模型,採用免疫組化檢測大腦的VCAM-1和ICAM-1錶達,免疫酶聯吸附法檢測血清的VCAM-1和ICAM-1濃度.結果 模型組大腦和血清的VCAM-1和ICAM-1均高于假手術組(P＜0.05).與模型組比較,丁苯酞預處理組大腦和血清的ICAM-1差異無統計學意義(P＞0.05),而丁苯酞預處理組大腦的VCAM-1水平[(0.127±0.017) ng/ml]和血清的VCAM-1水平[(160.22±53.95) ng/ml]低于模型組[大腦:(0.164±0.025) ng/ml;血清:(270.89 ± 76.49)ng/ml,P＜0.05].結論 丁苯酞具有抑製腦缺血再灌註大鼠大腦和血清VCAM-1的作用.
목적 탐색정분태대뇌결혈재관주대서적혈관세포점부분자-1(VCAM-1)화세포간점부분자-1(ICAM-1)적영향.방법 용수궤수표법장30지웅성SD대서분위3조:가수술조、모형조화정분태예처리조,매조10지;채용대뇌중동맥폐새법건립대서뇌결혈재관주모형,채용면역조화검측대뇌적VCAM-1화ICAM-1표체,면역매련흡부법검측혈청적VCAM-1화ICAM-1농도.결과 모형조대뇌화혈청적VCAM-1화ICAM-1균고우가수술조(P＜0.05).여모형조비교,정분태예처리조대뇌화혈청적ICAM-1차이무통계학의의(P＞0.05),이정분태예처리조대뇌적VCAM-1수평[(0.127±0.017) ng/ml]화혈청적VCAM-1수평[(160.22±53.95) ng/ml]저우모형조[대뇌:(0.164±0.025) ng/ml;혈청:(270.89 ± 76.49)ng/ml,P＜0.05].결론 정분태구유억제뇌결혈재관주대서대뇌화혈청VCAM-1적작용.
Objective To explore the effect of butylphthalide on vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in rats with middle cerebral artery ischemia-reperfusion.Methods 30 male SD rats were randomly divided into sham operation group(n=10),the model group(n=10) and butylphthalide pretreated group(n=10).Rat model of cerebral ischemia and reperfusion was established using the cerebral artery occlusion method.The brain expression of VCAM-1 and ICAM-1 was detected by immunohistochemistry.The serum VCAM-1 and ICAM-1 levels were detected using Enzyme-linked immunosorbent assay (ELISA).Results The VCAM-1 and ICAM-1 levels of brain and serum in the model group were higher than those of sham operation group (P＜0.05).The VCAM-1 expression of butylphthalide pretreatment group had no statistical difference with the model group (P＞0.05),while VCAM-1 expression was higher in butylphthalide pretreatment group compared with the model group (brain:(0.127 ± 0.017) ng/ml vs (0.164 ± 0.025) ng/ml; serum:(160.22±53.95) ng/ml vs (270.89±76.49) ng/ml,all P＜ 0.05).Conclusion Butylphthalide can inhibit the VCAM-1 level of brain and serum in rat with middle cerebral artery ischemia-reperfusion.
