CAJ | 학술논문

目的：研究塞来昔布在体外对人结肠癌细胞 COLO205生长增殖及对环氧化酶2（COX -2）、金属蛋白酶9（MMP -9）mRNA 表达的影响。方法体外培养人结肠癌细胞 COLO205，分组为正常对照组和塞来昔布干预组，MTT 法检测正常对照组和塞来昔布组抑制率，塞来昔布在不同时间且相同浓度的条件下，不同时间对于结肠癌细胞增殖的影响，测得抑制率并算得半抑制浓度（IC50）值；RT - PCR 检测 COX -2、MMP -9的 mRNA 的表达影响。结果 MTT 结果显示：相同浓度的塞来昔布干预结肠癌 COLO205细胞，分别在24 h，48 h，72 h 测算 IC50结果为：96.620±9.044μmol/ L、71.561±5.706μmol/ L、58.982±11.069μmol/ L，塞来昔布浓度提高和干预时间延长，结肠癌 COLO205细胞活力下降。RT - PCR 结果显示：正常对照组与塞来昔布干预组 COX -2 mRNA 灰度值分别为：0.832±0.012，0.113±0.001，干预组塞来昔布干预细胞的 COX -2mRNA 表达降低，所应显示的条带消失，无表达率，两组比较差异有显著性（ t =18.051，P =0.000）；正常对照组与塞来昔布干预组 MMP -9 mRNA 灰度值分别为：0.756±0.050，0.171±0.001，干预组 MMP -9 mRNA 表达明显降低，条带消失，无 mRNA 表达，两组比较差异有显著性（ t =17.286，P =0.001）。结论体外实验表明：塞来昔布抑制结肠癌 COLO205细胞增殖，通过抑制 COX -2及 MMP -9的 mRNA 的表达来实现抗肿瘤作用。
목적：연구새래석포재체외대인결장암세포 COLO205생장증식급대배양화매2（COX -2）、금속단백매9（MMP -9）mRNA 표체적영향。방법체외배양인결장암세포 COLO205，분조위정상대조조화새래석포간예조，MTT 법검측정상대조조화새래석포조억제솔，새래석포재불동시간차상동농도적조건하，불동시간대우결장암세포증식적영향，측득억제솔병산득반억제농도（IC50）치；RT - PCR 검측 COX -2、MMP -9적 mRNA 적표체영향。결과 MTT 결과현시：상동농도적새래석포간예결장암 COLO205세포，분별재24 h，48 h，72 h 측산 IC50결과위：96.620±9.044μmol/ L、71.561±5.706μmol/ L、58.982±11.069μmol/ L，새래석포농도제고화간예시간연장，결장암 COLO205세포활력하강。RT - PCR 결과현시：정상대조조여새래석포간예조 COX -2 mRNA 회도치분별위：0.832±0.012，0.113±0.001，간예조새래석포간예세포적 COX -2mRNA 표체강저，소응현시적조대소실，무표체솔，량조비교차이유현저성（ t =18.051，P =0.000）；정상대조조여새래석포간예조 MMP -9 mRNA 회도치분별위：0.756±0.050，0.171±0.001，간예조 MMP -9 mRNA 표체명현강저，조대소실，무 mRNA 표체，량조비교차이유현저성（ t =17.286，P =0.001）。결론체외실험표명：새래석포억제결장암 COLO205세포증식，통과억제 COX -2급 MMP -9적 mRNA 적표체래실현항종류작용。
Objective To study celecoxib in vitro human colon cancer cell growth and proliferation of COLO205 and its anti - tumor molecu-lar mechanisms. Methods Cultured human colon cancer COLO205,MTT method analyses celecoxib at the same concentration,at different times for the colon cancer cell were used to calculate IC50 value. RT - PCR method was used to evaluate the expression of COX -2,MMP -9 mRNA. Results MTT assay showed:the concentration of the same intervention celecoxib to inhibit the proliferation of human colon cancer cells,its 24 h,48 h,72 h IC50 of respectively:96. 620 ±9. 044 μmol/ L,71. 561 ± 5. 706 μmol/ L,58. 982 ± 11. 069 μmol/ L. The RT - PCR results show:human colon cancer cells COLO205 in normal control group and celecoxib in the intervention group,respectively. The COX -2mRNA gray value were:0. 832 ± 0. 012,0. 113 ±0. 001( t =18. 051,P =0. 000). MMP - 9 mRNA gray values were:0. 801 ± 0. 022,0. 102 ± 0. 001( t = 19. 037,P = 0. 000);MMP -9 mRNA were 0. 756 ±0. 050,0. 171 ±0. 001( t =17. 286,P = 0. 001). Conclusion Celecoxib inhibition of gastric cancer cell prolifera-tion,celecoxib antitumor mechanisms may by inhibiting the expression of COX -2 and MMP -9 mRNA in implementation.