CAJ | 학술논문

目的：研究在甲状腺癌细胞内外钙离子浓度改变时STIM1对SW579细胞的影响及其可能机制。方法用病毒转染法将带有STIM1‐YFP荧光探针的腺病毒载体（105病毒颗粒／细胞）转入SW579细胞24 h后，细胞计数绘制生长曲线；全内角反射荧光显微镜下观察SW579细胞在100μmol／L环匹阿尼酸（CPA）、100μmol／L卡巴胆碱干预下，STIM1在细胞膜附近的实时动态变化。结果 SW579细胞转染组与未转染组生长曲线无显著性差异（P＞0.05）；CPA处理后SW579细胞荧光强度增加到1.38±0.2（F／F0）；卡巴胆碱处理后，SW579细胞荧光增加到1.57±0.3（F／F0），有显著性差异（P＜0.01）。结论STIM1在SW579细胞内外钙离子浓度改变时表达并有活性，并可通过Ca2＋通道调节SW579细胞。
목적：연구재갑상선암세포내외개리자농도개변시STIM1대SW579세포적영향급기가능궤제。방법용병독전염법장대유STIM1‐YFP형광탐침적선병독재체（105병독과립／세포）전입SW579세포24 h후，세포계수회제생장곡선；전내각반사형광현미경하관찰SW579세포재100μmol／L배필아니산（CPA）、100μmol／L잡파담감간예하，STIM1재세포막부근적실시동태변화。결과 SW579세포전염조여미전염조생장곡선무현저성차이（P＞0.05）；CPA처리후SW579세포형광강도증가도1.38±0.2（F／F0）；잡파담감처리후，SW579세포형광증가도1.57±0.3（F／F0），유현저성차이（P＜0.01）。결론STIM1재SW579세포내외개리자농도개변시표체병유활성，병가통과Ca2＋통도조절SW579세포。
Objective To investigate the influence and molecular mechanism of STIM1 on SW579 thyroid cancer cells when calcium concentration change in SW579 thyroid cancer cells .Methods Using virus transfection method ,we transferred adenovi‐rus with STIM1‐YFP flurescent probe (105 viral particles per cell)into SW579 thyroid cancer cells ,and after 24 h .Using cell count to draw growth curve .Real‐time changes of STIM1‐YFP were observed by using total internal reflection fluorescence mi‐croscope under two different conditions :addition of 100 micromoles cyclopiazonic acid and 100 micromoles carbachol .Results There was no significant difference inSW579 between transfection groups and without transfection(P>0.05) .Fluorescence in‐tensity increased to 1.38 ± 0.2 (F/F0 ) after adding 100 micromoles CPA .Fluorescence intensity increased to 1.57 ± 0.3 (F/F0 ) after adding of 100 micromoles carbachol .There are significant differences (P<0.01) .Conclusion STIM1 is expressed and ac‐tive in adjust SW579 thyroid cancer cells and SW579 cells through the store‐operated calcium pathway .