CAJ | 학술논문

目的：探讨高脂低碳水化合物膳食模式对肥胖大鼠影响。方法利用膳食模式建立营养性肥胖大鼠模型100只，用随机数字表法按体重分层随机分为普通饲料对照组20只（9.0%脂肪，78%碳水化合物）、高脂饲料组80只（70%脂肪，16%碳水化合物）。每天称进食量；每周称大鼠体重；第3和第11周测血脂；第10周进行口服葡萄糖耐量试验和胰岛素释放试验、第11周测瘦素和酮体。结果两组大鼠原摄食标准为85 g/3 d，对照组大鼠3 d 内85 g 食物全部食用，观察组大鼠摄食量明显下降，摄入能量减少，且与对照组比较差异有统计学意义（ P ﹤0.05）；观察组大鼠高脂低碳水化合物膳食模式进行第28天、第35天、第42天、第56天、第70 d 体重增长明显，且与对照组同时间段比较差异均有统计学意义（ P ﹤0.05）；观察组大鼠高脂低碳水化合物膳食模式进行到第9周后甘油三酯（TG）、总胆固醇（TC）明显高于对照组同期水平、而高密度脂蛋白胆固醇（HDL - C）明显低于对照组，且差异均有统计学意义（ P ﹤0.05）；与对照组比较，观察组大鼠高脂低碳水化合物膳食模式进行70 d 后，体重、脂肪垫重、Lee 氏指数、脂肪体比等指标明显升高，脾体比明显降低，差异均有统计学意义（ P ﹤0.05）；观察组大鼠观察组在15 min 餐后血糖水平明显高于对照组，且与比较差异有统计学意义（ P ﹤0.05）；两组大鼠喂养70 d 后瘦素、酮体等指标比较差异无统计学意义（ P ﹥0.05）。结论高脂低碳水化合物膳食模式使大鼠对脂代谢的调节能力下降，出现血脂紊乱。
목적：탐토고지저탄수화합물선식모식대비반대서영향。방법이용선식모식건립영양성비반대서모형100지，용수궤수자표법안체중분층수궤분위보통사료대조조20지（9.0%지방，78%탄수화합물）、고지사료조80지（70%지방，16%탄수화합물）。매천칭진식량；매주칭대서체중；제3화제11주측혈지；제10주진행구복포도당내량시험화이도소석방시험、제11주측수소화동체。결과량조대서원섭식표준위85 g/3 d，대조조대서3 d 내85 g 식물전부식용，관찰조대서섭식량명현하강，섭입능량감소，차여대조조비교차이유통계학의의（ P ﹤0.05）；관찰조대서고지저탄수화합물선식모식진행제28천、제35천、제42천、제56천、제70 d 체중증장명현，차여대조조동시간단비교차이균유통계학의의（ P ﹤0.05）；관찰조대서고지저탄수화합물선식모식진행도제9주후감유삼지（TG）、총담고순（TC）명현고우대조조동기수평、이고밀도지단백담고순（HDL - C）명현저우대조조，차차이균유통계학의의（ P ﹤0.05）；여대조조비교，관찰조대서고지저탄수화합물선식모식진행70 d 후，체중、지방점중、Lee 씨지수、지방체비등지표명현승고，비체비명현강저，차이균유통계학의의（ P ﹤0.05）；관찰조대서관찰조재15 min 찬후혈당수평명현고우대조조，차여비교차이유통계학의의（ P ﹤0.05）；량조대서위양70 d 후수소、동체등지표비교차이무통계학의의（ P ﹥0.05）。결론고지저탄수화합물선식모식사대서대지대사적조절능력하강，출현혈지문란。
Objective To investigate the effects of high fat and low carbohydrate diet pattern in obese rats. Methods 100 rats models with nutritional obesity utilization of dietary patterns were randomly divided into the normal diet control group 20 only by random number table(9%fat,78% carbohydrate),high fat diet group was 80(70% fat,16% carbohydrate). The food intake amount was recorded every day. The body weight and serum lipids of rats at third and eleventh week were recorded. The OGTT test and IRT test at tenth week were recorded. The leptin and ketone body were measured at eleventh weeks. Results The two groups of rats feeding standard was 85 g/ 3 d,the rats of control group was 85 g within 3 days of food eaten. The observation group food intake of rats decreased significantly,reduced energy intake. And there was a statistically significant difference compared with the control group( P ﹤ 0. 05). The high fat and low carbohydrate diet model for twenty - eighth days,thirty- fifth days,forty - second days,fifty - sixth days,seventieth day of the observation group,weight gain significantly. The same time showed no significant differences( P ﹤ 0. 05)of the control group. TG,TC of the observation group rats of high fat and low carbohydrate diet mode for up to ninth weeks was significantly higher than that in the control group. While the level of HDL - C of the same period was significantly lower than that of the control group,and the difference is statistically significant( P ﹤ 0. 05). The high fat and low carbohydrate dietary patterns of the observa-tion group of rats were 70 days. The body weight,fat pad weight,Leeˊs index,spleen body ratio,body fat ratio were compared with the control group. The differences were statistically significant( P ﹤ 0. 05)different from the observation group. The postprandial blood glucose levels of the rats in the observation group were significantly higher than those in the control group,and there was significant difference( P ﹤ 0. 05). The two group rats were fed for 70 days of leptin,ketone index comparison difference has no statistical significance( P ﹥ 0 05). Conclusion High fat and low carbohydrate diet model rats decreased ability to adjust lipid metabolism,lipid disorders.