CAJ | 학술논문

目的研究大鼠肌紧张带(TB)重复低频电刺激疲劳试验后的生物力学及病理学改变.方法符合实验标准的28只Wistar大鼠,按随机数字表法分为对照组、强度疲劳实验组和频率疲劳实验组,分别进行对照、强度疲劳实验和频率疲劳实验研究.记录和分析TB和非肌紧张带(non-TB)在每一刺激循环中的最大收缩力和最大收缩力低刺激强度和高刺激强度,以及频率疲劳实验中的最大收缩力、强直收缩力和频率.光镜下观察其病理变化.结果 ①强度疲劳实验中,TB的最大收缩力在第15次循环(1.42±0.28)g和第20次循环(0.93±0.54)g中明显比其第1次循环(1.99 ±0.31)g和第5次循环(1.97±0.27)g降低;最大收缩力高刺激强度在第15次循环(3.76±0.71)V和第20次循环(3.44±0.97)V中明显比其第1次循环(4.04±0.64)V降低.在第10、15和20次强度刺激循环中,TB的最大收缩力和最大收缩力高刺激强度明显比non-TB低.②频率疲劳实验中,TB最大收缩力频率(9.60 ±2.12)Hz和强直频率(25.45±2.65)Hz明显小于non-TB,最大收缩力(1.67±0.16)g和强直收缩力(2.02±0.21)g明显大于non-TB.③病理切片显微镜观察显示强度和频率疲劳实验后,TB肌纤维染色明显不均,肌纤维排列紊乱,出现明显的水肿、细胞退变和断裂.结论 TB耐受不同电刺激强度和频率的能力降低,肌纤维易受损伤,肌肉抗疲劳能力下降;TB或许参与肌筋膜疼痛综合征患者的肌无力和易疲劳性.
목적 연구대서기긴장대(TB)중복저빈전자격피로시험후적생물역학급병이학개변.방법 부합실험표준적28지Wistar대서,안수궤수자표법분위대조조、강도피로실험조화빈솔피로실험조,분별진행대조、강도피로실험화빈솔피로실험연구.기록화분석TB화비기긴장대(non-TB)재매일자격순배중적최대수축력화최대수축력저자격강도화고자격강도,이급빈솔피로실험중적최대수축력、강직수축력화빈솔.광경하관찰기병리변화.결과 ①강도피로실험중,TB적최대수축력재제15차순배(1.42±0.28)g화제20차순배(0.93±0.54)g중명현비기제1차순배(1.99 ±0.31)g화제5차순배(1.97±0.27)g강저;최대수축력고자격강도재제15차순배(3.76±0.71)V화제20차순배(3.44±0.97)V중명현비기제1차순배(4.04±0.64)V강저.재제10、15화20차강도자격순배중,TB적최대수축력화최대수축력고자격강도명현비non-TB저.②빈솔피로실험중,TB최대수축력빈솔(9.60 ±2.12)Hz화강직빈솔(25.45±2.65)Hz명현소우non-TB,최대수축력(1.67±0.16)g화강직수축력(2.02±0.21)g명현대우non-TB.③병리절편현미경관찰현시강도화빈솔피로실험후,TB기섬유염색명현불균,기섬유배렬문란,출현명현적수종、세포퇴변화단렬.결론 TB내수불동전자격강도화빈솔적능력강저,기섬유역수손상,기육항피로능력하강;TB혹허삼여기근막동통종합정환자적기무력화역피로성.
Objective To investigate the biomechanical and pathological changes in vivo in the taut bands (TB) of biceps femoris in rats after repeated low-frequency electrical stimulation.Methods Twenty-eight Wistar rats were randomly divided into a control group,an electrical intensity-dependent fatigue group,which were subject to electric intensity-dependent fatigue test,and an electrical frequency-dependent fatigue group,which were subject to electrical frequency-dependent fatigue test.After fatigue tests,the taut band of the biceps femoris and the non-taut band of the contralateral biceps femoris were harvested for pathological observation.The maximum contraction force (MCF),electrical intensity-and frequency-dependent fatigue characteristics and any pathological changes in the TBs were assessed and compared to the non-taut band region of the other biceps femoris.Results The MCF at the 15th and 20th stimulation (1.42 ± 0.28 g and 0.93 ± 0.54 g respectively) were significantly lower than that at the 1 st and 5th stimulation of the TBs.High stimulation intensity (HSI) at the 15th and 20th stimulation (3.76 ± 0.71 V and 3.44 ± 0.97 V) were also significantly lower than at the 1st TB stimulation.At the 10th,15th and 20th stimulation of the TBs,MCF and HSI were both significantly lower than in the bands which were not tight.In the frequency-dependent fatigue stimulation tests,the frequency which generated the MCF of the TBs was significantly lower than in the bands which were not tight,while the MCF of the TBs was significantly higher than that of non-TBs.After either intensity or frequency fatigue testing,more severe edema,uneven cytoplasmic death and degeneration of muscle fibers were observed in sections from TBs than from the bands which were not tight.Conclusions Taut muscle bands are significantly less fatigue-resistant than normal muscle fibers.Taut bands may contribute to the fatigue of myofascial pain syndromes.