检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2015年
16期
2406-2407
,共2页
高脂血症%生化测定%干扰
高脂血癥%生化測定%榦擾
고지혈증%생화측정%간우
hyperlipidemia%biochemical measurement%interference
目的:探讨高速离心法、稀释法、乙醚萃取法消除血脂干扰的效果,为消除血脂对生化检测的干扰提供参考。方法收集100例血脂标本,将标本分为4份,每组25份,其中3组分别给予高速离心法、稀释法、乙醚萃取法处理,另1组原血浆检测。比较处理前后各组间的差异,并对高速离心法、稀释法、乙醚萃取法组采用方差分析。结果3种方法对Ca2+、尿酸(UA)、肌酐(Cr)、血糖(GLU)、清蛋白(ALB)、总蛋白(TP)、直接胆红素(DBIL)、总胆红素(TBIL)、淀粉酶(AMY)、肌酸激酶同工酶(CK‐MB)、肌酸激酶(CK)、羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)、谷氨酰基转移酶(γGT)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)检测值处理前后,差异有统计学意义(P<0.05),检测值由高到低依次为血清原液检测、离子水稀释法、低温高速离心法、乙醚萃取法。结论乙醚萃取法对蛋白质类检测项目影响明显,离子水稀释法受其影响最低,在临床工作中3种方法可联合使用。
目的:探討高速離心法、稀釋法、乙醚萃取法消除血脂榦擾的效果,為消除血脂對生化檢測的榦擾提供參攷。方法收集100例血脂標本,將標本分為4份,每組25份,其中3組分彆給予高速離心法、稀釋法、乙醚萃取法處理,另1組原血漿檢測。比較處理前後各組間的差異,併對高速離心法、稀釋法、乙醚萃取法組採用方差分析。結果3種方法對Ca2+、尿痠(UA)、肌酐(Cr)、血糖(GLU)、清蛋白(ALB)、總蛋白(TP)、直接膽紅素(DBIL)、總膽紅素(TBIL)、澱粉酶(AMY)、肌痠激酶同工酶(CK‐MB)、肌痠激酶(CK)、羥丁痠脫氫酶(HBDH)、乳痠脫氫酶(LDH)、穀氨酰基轉移酶(γGT)、天門鼕氨痠氨基轉移酶(AST)、丙氨痠氨基轉移酶(ALT)檢測值處理前後,差異有統計學意義(P<0.05),檢測值由高到低依次為血清原液檢測、離子水稀釋法、低溫高速離心法、乙醚萃取法。結論乙醚萃取法對蛋白質類檢測項目影響明顯,離子水稀釋法受其影響最低,在臨床工作中3種方法可聯閤使用。
목적:탐토고속리심법、희석법、을미췌취법소제혈지간우적효과,위소제혈지대생화검측적간우제공삼고。방법수집100례혈지표본,장표본분위4빈,매조25빈,기중3조분별급여고속리심법、희석법、을미췌취법처리,령1조원혈장검측。비교처리전후각조간적차이,병대고속리심법、희석법、을미췌취법조채용방차분석。결과3충방법대Ca2+、뇨산(UA)、기항(Cr)、혈당(GLU)、청단백(ALB)、총단백(TP)、직접담홍소(DBIL)、총담홍소(TBIL)、정분매(AMY)、기산격매동공매(CK‐MB)、기산격매(CK)、간정산탈경매(HBDH)、유산탈경매(LDH)、곡안선기전이매(γGT)、천문동안산안기전이매(AST)、병안산안기전이매(ALT)검측치처리전후,차이유통계학의의(P<0.05),검측치유고도저의차위혈청원액검측、리자수희석법、저온고속리심법、을미췌취법。결론을미췌취법대단백질류검측항목영향명현,리자수희석법수기영향최저,재림상공작중3충방법가연합사용。
Objective To investigate the effects of high‐speed centrifugation ,dilution and ether extraction methods in eliminating the blood lipid interference to provide reference for eliminating this interference .Methods 100 cases of lipid samples ,the specimens were divided into 4 parts ,1 part of each group ,three groups were given high‐speed centrifugation ,dilution and ether extraction methods ,and another group was detected as the original plas‐ma .The differences before and after processing were compared among groups .The high‐speed centrifugation ,dilution and ether extraction methods adopted the variance analysis .Results Ca2+ ,UA ,Cr ,GLU ,ALB ,TP ,DBIL ,TBIL , AMY ,CK‐MB ,CK ,HBDH ,LDH ,γGT ,AST and ALT values detected by 3 kinds of method had statistically signifi‐cant differences between before and after processing (P<0 .05) .The detection value in descending order of serum dope testing ,deionized water dilution ,low‐speed centrifugation ,ether extraction .Conclusion The ether extraction method significantly affects the protein detection items ,the influence of ionized water dilution is minimum ,three kinds of method can be combined for use in clinical work .