中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2015年
7期
488-492
,共5页
王勇%任佰绪%吴书峰%钟琴%李小俚%路承彪
王勇%任佰緒%吳書峰%鐘琴%李小俚%路承彪
왕용%임백서%오서봉%종금%리소리%로승표
经颅超声刺激%帕金森病%丙二醛%谷胱甘肽过氧化物
經顱超聲刺激%帕金森病%丙二醛%穀胱甘肽過氧化物
경로초성자격%파금삼병%병이철%곡광감태과양화물
Ultrasound%Parkinson's disease%Motor function%Antioxidants%Brain
目的 探讨经颅超声刺激(TUS)对帕金森病(PD)小鼠运动功能及抗氧化能力的影响.方法 选择近交系C57BL雄性小鼠32只,按照随机数字表法将其分为正常对照组、模型组、假超声刺激组、超声刺激组,每组8只.模型组、假超声刺激组、超声刺激组采用1-甲基-4苯基-1,2,3,6-四氢吡啶(MPTP)腹腔注射(20 mg/kg)制备PD模型,正常对照组给予生理盐水.造模成功后,采用0.5 MHz、1 W/cm2低强度聚焦超声波(LIFU)穿过超声刺激组小鼠颅骨刺激中脑黑质区,将超声探头置于假超声刺激组小鼠头皮上、关闭超声波.造模前、造模2周后、造模5周后对各组小鼠进行爬杆测试.造模5周后处死大鼠,测定全脑丙二醛(M DA)、谷胱甘肽过氧化物(GSH-Px)含量.结果 造模前,各组小鼠运动功能评分之间比较,差异无统计学意义(P＞0.05).与组内造模前比较,模型组[(4.30±1.19)分]、假超声刺激组[(4.40 ±0.23)分]、超声刺激组[(4.80±0.23)分]造模2周后运动功能评分显著降低(P＜0.05),模型组[(5.12±0.83)分]、假超声刺激组[(5.51±1.21)分]造模5周后运动功能评分亦较低(P＜0.05).与组内造模2周后比较,超声刺激组造模5周后运动功能评分[(6.69±1.11)分]显著升高(P＜0.05).与正常对照组比较,其余3组造模2周后运动功能评分较低(P＜0.05),模型组、假超声刺激组造模5周后运动功能评分较低(P＜0.05).与模型组比较,超声刺激组造模5周后运动功能评分较高(P＜0.05).与假超声刺激组比较,超声刺激组造模5周后运动功能评分较高(P＜0.05).造模5周后,模型组[(10.2±1.1) nmol/ml]、假超声刺激组[(9.4±1.3) nmol/ml] MDA含量较正常对照组[(4.5±0.8)nmol/ml]显著升高(P＜0.05),超声刺激组MDA含量[(6.8±0.9)nmol/ml]较模型组、假超声刺激组降低(P＜0.05).造模5周后,模型组[(100.0±35.4) U/mgprot]、假超声刺激组[(444.0±24.9) U/mgprot] GSH-Px酶活力水平较正常对照组[(1262.5±53.0) U/mgprot]显著降低(P＜0.05),超声刺激组[(1047.3±77.8) U/mgprot] GSH-Px酶活力水平较模型组、假超声刺激组升高(P＜0.05).结论 TUS可改善PD小鼠的运动功能,其机制可能与TUS增强其抗氧化能力有关.
目的 探討經顱超聲刺激(TUS)對帕金森病(PD)小鼠運動功能及抗氧化能力的影響.方法 選擇近交繫C57BL雄性小鼠32隻,按照隨機數字錶法將其分為正常對照組、模型組、假超聲刺激組、超聲刺激組,每組8隻.模型組、假超聲刺激組、超聲刺激組採用1-甲基-4苯基-1,2,3,6-四氫吡啶(MPTP)腹腔註射(20 mg/kg)製備PD模型,正常對照組給予生理鹽水.造模成功後,採用0.5 MHz、1 W/cm2低彊度聚焦超聲波(LIFU)穿過超聲刺激組小鼠顱骨刺激中腦黑質區,將超聲探頭置于假超聲刺激組小鼠頭皮上、關閉超聲波.造模前、造模2週後、造模5週後對各組小鼠進行爬桿測試.造模5週後處死大鼠,測定全腦丙二醛(M DA)、穀胱甘肽過氧化物(GSH-Px)含量.結果 造模前,各組小鼠運動功能評分之間比較,差異無統計學意義(P＞0.05).與組內造模前比較,模型組[(4.30±1.19)分]、假超聲刺激組[(4.40 ±0.23)分]、超聲刺激組[(4.80±0.23)分]造模2週後運動功能評分顯著降低(P＜0.05),模型組[(5.12±0.83)分]、假超聲刺激組[(5.51±1.21)分]造模5週後運動功能評分亦較低(P＜0.05).與組內造模2週後比較,超聲刺激組造模5週後運動功能評分[(6.69±1.11)分]顯著升高(P＜0.05).與正常對照組比較,其餘3組造模2週後運動功能評分較低(P＜0.05),模型組、假超聲刺激組造模5週後運動功能評分較低(P＜0.05).與模型組比較,超聲刺激組造模5週後運動功能評分較高(P＜0.05).與假超聲刺激組比較,超聲刺激組造模5週後運動功能評分較高(P＜0.05).造模5週後,模型組[(10.2±1.1) nmol/ml]、假超聲刺激組[(9.4±1.3) nmol/ml] MDA含量較正常對照組[(4.5±0.8)nmol/ml]顯著升高(P＜0.05),超聲刺激組MDA含量[(6.8±0.9)nmol/ml]較模型組、假超聲刺激組降低(P＜0.05).造模5週後,模型組[(100.0±35.4) U/mgprot]、假超聲刺激組[(444.0±24.9) U/mgprot] GSH-Px酶活力水平較正常對照組[(1262.5±53.0) U/mgprot]顯著降低(P＜0.05),超聲刺激組[(1047.3±77.8) U/mgprot] GSH-Px酶活力水平較模型組、假超聲刺激組升高(P＜0.05).結論 TUS可改善PD小鼠的運動功能,其機製可能與TUS增彊其抗氧化能力有關.
목적 탐토경로초성자격(TUS)대파금삼병(PD)소서운동공능급항양화능력적영향.방법 선택근교계C57BL웅성소서32지,안조수궤수자표법장기분위정상대조조、모형조、가초성자격조、초성자격조,매조8지.모형조、가초성자격조、초성자격조채용1-갑기-4분기-1,2,3,6-사경필정(MPTP)복강주사(20 mg/kg)제비PD모형,정상대조조급여생리염수.조모성공후,채용0.5 MHz、1 W/cm2저강도취초초성파(LIFU)천과초성자격조소서로골자격중뇌흑질구,장초성탐두치우가초성자격조소서두피상、관폐초성파.조모전、조모2주후、조모5주후대각조소서진행파간측시.조모5주후처사대서,측정전뇌병이철(M DA)、곡광감태과양화물(GSH-Px)함량.결과 조모전,각조소서운동공능평분지간비교,차이무통계학의의(P＞0.05).여조내조모전비교,모형조[(4.30±1.19)분]、가초성자격조[(4.40 ±0.23)분]、초성자격조[(4.80±0.23)분]조모2주후운동공능평분현저강저(P＜0.05),모형조[(5.12±0.83)분]、가초성자격조[(5.51±1.21)분]조모5주후운동공능평분역교저(P＜0.05).여조내조모2주후비교,초성자격조조모5주후운동공능평분[(6.69±1.11)분]현저승고(P＜0.05).여정상대조조비교,기여3조조모2주후운동공능평분교저(P＜0.05),모형조、가초성자격조조모5주후운동공능평분교저(P＜0.05).여모형조비교,초성자격조조모5주후운동공능평분교고(P＜0.05).여가초성자격조비교,초성자격조조모5주후운동공능평분교고(P＜0.05).조모5주후,모형조[(10.2±1.1) nmol/ml]、가초성자격조[(9.4±1.3) nmol/ml] MDA함량교정상대조조[(4.5±0.8)nmol/ml]현저승고(P＜0.05),초성자격조MDA함량[(6.8±0.9)nmol/ml]교모형조、가초성자격조강저(P＜0.05).조모5주후,모형조[(100.0±35.4) U/mgprot]、가초성자격조[(444.0±24.9) U/mgprot] GSH-Px매활력수평교정상대조조[(1262.5±53.0) U/mgprot]현저강저(P＜0.05),초성자격조[(1047.3±77.8) U/mgprot] GSH-Px매활력수평교모형조、가초성자격조승고(P＜0.05).결론 TUS가개선PD소서적운동공능,기궤제가능여TUS증강기항양화능력유관.
Objective To investigate the effects of transcranial ultrasound stimulation (TUS) on the motor functioning and anti-oxidative capacity of mice with Parkinson's disease (PD).Methods Thirty-two inbred C57BL male mice were randomized into a normal control group,a model group,a sham TUS group and a TUS group (n =8 for each group) according to a random number table.A PD model was induced in the mice of the model,sham TUS and TUS groups by injecting 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 20 mg/kg intraperitoneally,while those in the normal control group were given saline.Low intensity (1 W) focused ultrasound (LIFU) at a frequency of 0.5 MHz was then applied to stimulate the nigra region,except for the mice in the sham TUS group,which were treated with the same procedure but with no ultrasound output.A pole climbing test was carried out before,2 weeks and 5 weeks after the injection of the MPTP.After 5 weeks the animals were sacrificed and the whole brain malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) content were measured.Results No significant differences in in pole climbing scores were observed among the four groups before the MPTP injections.However,the average value decreased significantly to (4.30 ± 1.19),(4.40 ± 0.23) and (4.80 ± 0.23) for the model,sham TUS and TUS groups respectively 2 weeks after the injection.It then rose to (5.12 ±0.83) and (5.51 ± 1.21)for the first two groups 3 weeks later,but was still lower that before the injection.After 5 weeks the TUS group's average score was significantly higher than 3 weeks earlier and than that of the model group and the sham TUS group.Compared with the control group,the other groups' average scores were all lower 2 weeks after MPTP injection,and those of the model and the sham TUS groups remained so 5 weeks after the injection.Five weeks after the injection,the average MDA content of the model group (10.2 ± 1.1 nmol/ml) and the sham TUS group (9.4 ±1.3 nmol/ml) were significantly higher than the normal control group (4.5 ± 0.8 nmol/ml),as well as the TUS group (6.8 ± 0.9 nmol/ml).However,GSH-Px enzyme activity in the model group (100 ± 35.4 U/mgprot) and the sham US group (444 ± 24.9 U/mgprot) was significantly lower than that in the normal control group (1262.5 ± 53 U/mgprot),together with the TUS group (1047.3 ± 77.8 U/mgprot).Conclusion TUS can improve motor function in PD,at least in mice.This may be due to its anti-oxidative capacity.
