化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2015年
4期
44-47
,共4页
艾叶%气相色谱法%桉油精%樟脑%薄荷脑%龙脑
艾葉%氣相色譜法%桉油精%樟腦%薄荷腦%龍腦
애협%기상색보법%안유정%장뇌%박하뇌%용뇌
artemisia argyi%gas chromatography%eucalyptol%camphor%menthol%borneol
建立同时测定艾叶中桉油精、樟脑、薄荷脑、龙脑4种挥发性成分含量的分析方法。样品采用水蒸气蒸馏法提取。色谱柱采用DB–WAX毛细管柱(30 m×0.32 mm，0.25μm)；载气为氮气(99.99%)，分流比为10∶1；进样口温度为240℃；升温程序：柱温起始温度70℃，保持1 min，然后以5℃／min的速率升温至150℃，保持2 min，再以10℃／min的速率升温至200℃，保持6 min；氢火焰离子化检测器(FID)温度为240℃。桉油精的质量浓度在0.05～0.8 mg／mL范围内与色谱峰面积呈良好的线性，相关系数r＞0.999；樟脑、薄荷脑、龙脑的质量浓度在0.01～0.2 mg／mL范围内与色谱峰面积线性关系良好，相关系数r＞0.999；方法回收率为92.00%～98.40%，测定结果的相对标准偏差为1.58%～2.44%(n=6)。桉油精、樟脑、薄荷脑、龙脑检出限分别为0.66，0.76，0.81，0.83 mg／kg。该方法操作简单，准确度与灵敏度高，适合同时测定艾叶中桉油精、樟脑、薄荷脑、龙脑4个挥发性成分含量。
建立同時測定艾葉中桉油精、樟腦、薄荷腦、龍腦4種揮髮性成分含量的分析方法。樣品採用水蒸氣蒸餾法提取。色譜柱採用DB–WAX毛細管柱(30 m×0.32 mm，0.25μm)；載氣為氮氣(99.99%)，分流比為10∶1；進樣口溫度為240℃；升溫程序：柱溫起始溫度70℃，保持1 min，然後以5℃／min的速率升溫至150℃，保持2 min，再以10℃／min的速率升溫至200℃，保持6 min；氫火燄離子化檢測器(FID)溫度為240℃。桉油精的質量濃度在0.05～0.8 mg／mL範圍內與色譜峰麵積呈良好的線性，相關繫數r＞0.999；樟腦、薄荷腦、龍腦的質量濃度在0.01～0.2 mg／mL範圍內與色譜峰麵積線性關繫良好，相關繫數r＞0.999；方法迴收率為92.00%～98.40%，測定結果的相對標準偏差為1.58%～2.44%(n=6)。桉油精、樟腦、薄荷腦、龍腦檢齣限分彆為0.66，0.76，0.81，0.83 mg／kg。該方法操作簡單，準確度與靈敏度高，適閤同時測定艾葉中桉油精、樟腦、薄荷腦、龍腦4箇揮髮性成分含量。
건립동시측정애협중안유정、장뇌、박하뇌、용뇌4충휘발성성분함량적분석방법。양품채용수증기증류법제취。색보주채용DB–WAX모세관주(30 m×0.32 mm，0.25μm)；재기위담기(99.99%)，분류비위10∶1；진양구온도위240℃；승온정서：주온기시온도70℃，보지1 min，연후이5℃／min적속솔승온지150℃，보지2 min，재이10℃／min적속솔승온지200℃，보지6 min；경화염리자화검측기(FID)온도위240℃。안유정적질량농도재0.05～0.8 mg／mL범위내여색보봉면적정량호적선성，상관계수r＞0.999；장뇌、박하뇌、용뇌적질량농도재0.01～0.2 mg／mL범위내여색보봉면적선성관계량호，상관계수r＞0.999；방법회수솔위92.00%～98.40%，측정결과적상대표준편차위1.58%～2.44%(n=6)。안유정、장뇌、박하뇌、용뇌검출한분별위0.66，0.76，0.81，0.83 mg／kg。해방법조작간단，준학도여령민도고，괄합동시측정애협중안유정、장뇌、박하뇌、용뇌4개휘발성성분함량。
A method was established for determinations of four volatile components (eucalyptol,camphor,menthol and borneol) in artemisia argyi.The target analytes of artemisia argyi were extracted by steam distillation,and separated by programmed temperature with Agilent DB–WAX capillary column(30 m×0.32 mm×0.25μm). The carrier gas was nitrogen(99.99%),split ratio was 10∶1. The inlet temperature was 240℃.The column temperature program:the initial temperature was 70℃,maintained for 1 min,raised to 150℃with rate of 5℃/min,maintained for 2 min,and at last raised to 200℃with rate of 10℃/min,maintained for 6 min. The FID detector temperature was 240℃. The concentration of eucalyptol was linear with peak area in the range of 0.05–0.8 mg/mL. The concentration of camphor,menthol and borneol was linear with peak area in the range of 0.01–0.2 mg/mL. The correlation coefficient was more than 0.999. The recovery rates ranged from 92.00%to 98.40%,with relative standard deviations of 1.58%–2.44%(n=6). The detection limits of eucalyptol,camphor,menthol and borneol were 0.66,0.76,0.81,0.83 mg/kg,respectively. This method is simple to operate with high accuracy and sensitivity. It is suitable for simultaneous determination of four volatile components in artemisia argyi.
