CAJ | 학술논문

目的：评定在乳酸脱氢酶（LDH）催化活性浓度测量中不同厂家或批号试剂的不确定度。方法配制不同厂家或批号LDH试剂，用国际临床化学联合会（IFCC）参考方法分别检测中值和高值两个浓度的混合血清，对所得数据进行配对 t检验，计算不同厂家或批号试剂间的不确定度。结果 P＜0．05表示对不同厂家或批号LDH试剂，所测LDH催化活性浓度结果差异有统计学意义。不同试剂批号对中值和高值混合血清的扩展不确定度U分别为6．52％（k＝2）和6．78％（k＝2）。结论不同厂家或批号试剂对LDH催化活性测量有影响，因此，在进行不确定度评定时，应考虑不同厂家或批号试剂的影响。
목적：평정재유산탈경매（LDH）최화활성농도측량중불동엄가혹비호시제적불학정도。방법배제불동엄가혹비호LDH시제，용국제림상화학연합회（IFCC）삼고방법분별검측중치화고치량개농도적혼합혈청，대소득수거진행배대 t검험，계산불동엄가혹비호시제간적불학정도。결과 P＜0．05표시대불동엄가혹비호LDH시제，소측LDH최화활성농도결과차이유통계학의의。불동시제비호대중치화고치혼합혈청적확전불학정도U분별위6．52％（k＝2）화6．78％（k＝2）。결론불동엄가혹비호시제대LDH최화활성측량유영향，인차，재진행불학정도평정시，응고필불동엄가혹비호시제적영향。
Objective To evaluate the measurement uncertainty of lactate dehydrogenase(LDH ) catalytic ac‐tivity concentration with different manufacturers or different lot numbers .Methods The LDH reagents with differ‐ent manufacturers or different lot numbers were prepared .The pooled serum with low or high concentrations was measured by the reference method recommended by International Federation of Clinical Chemistry and laboratory medicine(IFCC) .The experimental data were analyzed by paired t test .The measurement uncertainty of lactate dehy‐drogenase with different manufacturers or different lot numbers was calculated .Results P<0 .05 indicated that the differences of detected LDH catalytic activity concentrations results for different manufacturers or lot numbers of LDH reagents had statistical significance .The expanded uncertainty (U) of different manufacturers or lot numbers of reagents were 6 .52% (k=2) for low concentration and 6 .78% (k=2) for high concentration .Conclusion Different manufacturers or different lot numbers of reagents have significant effect on the catalytic activity concentrations of LDH .Therefore ,in the detection of measurement uncertainty ,the impact of different manufacturers or different lot numbers of reagents should be considered .