CAJ | 학술논문

目的：通过比较不同温度保存的自制混合血浆与正常混合血浆对血浆纠正试验的影响，探讨自制混合血浆在血浆纠正试验中的临床应用价值。方法收集凝血酶原时间（PT）≥18．0s和（或）活化部分凝血活酶时间（APTT）≥52．0s的血浆标本35份，分别与正常混合血浆（A组）、－80℃（B组）和－20℃（C组）保存的自制混合血浆按体积1∶1混合后做血浆纠正试验，即混合后立即测定PT和（或）APTT，37℃孵育1h后再次检测。计算35份标本可被正常混合血浆纠正的比例及3组的纠正率。结果88．57％（31／35）可被正常混合血浆立即纠正，4例未被纠正，其中1例孵育后比孵育前测定时间更加延长，占2．86％；3例孵育前后测定结果不变，占8．57％。在PT延长的血浆纠正试验中，各组之间纠正率差异无统计学意义（P＞0．05）。在APTT延长的纠正试验中，孵育前A组、B组和C组的纠正率分别为（32．93±16．17）％、（27．56±23．48）％和（13．70±12．87）％，A组和B组之间纠正率差异无统计学意义（P＞0．05），其余各组之间纠正率差异有统计学意义（P＜0．05）。结论－80℃保存的自制混合血浆可代替商品化的正常混合血浆进行血浆纠正试验。
목적：통과비교불동온도보존적자제혼합혈장여정상혼합혈장대혈장규정시험적영향，탐토자제혼합혈장재혈장규정시험중적림상응용개치。방법수집응혈매원시간（PT）≥18．0s화（혹）활화부분응혈활매시간（APTT）≥52．0s적혈장표본35빈，분별여정상혼합혈장（A조）、－80℃（B조）화－20℃（C조）보존적자제혼합혈장안체적1∶1혼합후주혈장규정시험，즉혼합후립즉측정PT화（혹）APTT，37℃부육1h후재차검측。계산35빈표본가피정상혼합혈장규정적비례급3조적규정솔。결과88．57％（31／35）가피정상혼합혈장립즉규정，4례미피규정，기중1례부육후비부육전측정시간경가연장，점2．86％；3례부육전후측정결과불변，점8．57％。재PT연장적혈장규정시험중，각조지간규정솔차이무통계학의의（P＞0．05）。재APTT연장적규정시험중，부육전A조、B조화C조적규정솔분별위（32．93±16．17）％、（27．56±23．48）％화（13．70±12．87）％，A조화B조지간규정솔차이무통계학의의（P＞0．05），기여각조지간규정솔차이유통계학의의（P＜0．05）。결론－80℃보존적자제혼합혈장가대체상품화적정상혼합혈장진행혈장규정시험。
Objective To explore the clinical application value of homemade plasma in the plasma correct test by comparing the influence of normal mixture plasma and homemade plasma storage at different temperatures .Meth‐ods 35 plasma samples of prothrombin time(PT)≥18 .0 s and/or activated partial thromboplastin time (APTT)≥52 .0 s were collected and mixed with the normal mixture plasma(group A) ,homemade plasma stored at -80 ℃(group B) and -20 ℃ (group C) with the proportion of 1∶1 respectively ,then PT and APTT were tested immedi‐ately ,and retested after 37 ℃ incubating for 1 h ,finally the correcting proportion in 35 samples and the correct ratio of these three groups were calculated .Results In the 35 cases of PT and/or APTT prolongation ,31 cases (88 .57% ) could be corrected by the normal mixture plasma and 4 cases were not corrected ,among them ,the detection time after incubation in 1 case(2 .86% ) was prolonged than that before incubation ,and the detection results before and after in‐cubation in 3 cases were unchanged ,accounting for 8 .57% .In the PT prolonged correction test ,there was no statisti‐cal difference in the correct ratio among 3 groups (P>0 .05) .In the APTT prolonged correction test ,the correcting ratios before incubation in the group A ,B and C were (32 .93 ± 16 .17)% ,(27 .56 ± 23 .48)% and (13 .70 ± 12 .87)%respectively ,there was no statistical difference in the correct ratio between the group A and B (P>0 .05) ,which in the other groups had statistical difference(P<0 .05) .Conclusion The homemade plasma stored at -80 ℃ can re‐place the commercialization normal mixture plasma for conducting the plasma correcting test .