CAJ | 학술논문

目的：探讨血清胸苷激酶1（sTK1）在儿童急性淋巴细胞白血病临床诊疗中的应用。方法采用免疫印迹增强化学发光法检测39例急性淋巴细胞白血病儿童患者化疗前、化疗后的sTK1浓度及30例同期健康体检儿童的sTK1浓度。结果患儿组化疗前的sTK1浓度明显高于化疗后完全缓解组、部分缓解组及对照组，差异有统计学意义（P＜0．01）；化疗后不同疗效组（完全缓解组、部分缓解组、未缓解组）之间sTK1浓度差异有统计学意义（P＜0．01）；未缓解组与化疗前的sTK1浓度差异无统计学意义（P＞0．05）；化疗前sTK1阳性检出率明显高于化疗后及对照组，差异有统计学意义（P＜0．01）。结论 sTK1的检测对于儿童急性淋巴细胞白血病的筛查、辅助诊断、化疗效果的评估具有重要参考价值。
목적：탐토혈청흉감격매1（sTK1）재인동급성림파세포백혈병림상진료중적응용。방법채용면역인적증강화학발광법검측39례급성림파세포백혈병인동환자화료전、화료후적sTK1농도급30례동기건강체검인동적sTK1농도。결과환인조화료전적sTK1농도명현고우화료후완전완해조、부분완해조급대조조，차이유통계학의의（P＜0．01）；화료후불동료효조（완전완해조、부분완해조、미완해조）지간sTK1농도차이유통계학의의（P＜0．01）；미완해조여화료전적sTK1농도차이무통계학의의（P＞0．05）；화료전sTK1양성검출솔명현고우화료후급대조조，차이유통계학의의（P＜0．01）。결론 sTK1적검측대우인동급성림파세포백혈병적사사、보조진단、화료효과적평고구유중요삼고개치。
Objective To study the clinical application of serum thymidine kinase 1 (sTK1) in the diagnosis and treatment of childhood acute lymphoblastic leukemia .Methods The Western blot‐enhanced chemiluminescence assay was adopted to detect serum TK1 level in 39 cases of childhood acute lymphoblastic leukemia patients before and after chemotherapy and contemporaneous 30 healthy children .Results The sTK1 level before chemotherapy in the patients group was higher than that in the complete remission group and the partial remission group after chemo‐therapy ,and the healthy control group ,the differences were statistically significant(P< 0 .01);the sTK1 level had statistical differences among different curative effect groups after chemotherapy(complete remission group ,partial re‐mission group and non‐remission group) (P<0 .01);the sTK1 level had no statistical difference between the non‐re‐mission group and before chemotherapy (P>0 .05);the positive detection rate of sTK1 before chemotherapy was sig‐nificantly higher than that after chemotherapy and the healthy control group(P<0 .01) .Conclusion The sTK1 de‐tection has the important reference value for screening ,assisted diagnosis and evaluation of chemotherapeutic effect in childhood acute lymphoblastic leukemia .