CAJ | 학술논문

目的：分析 A2亚型频率分布和基因分型的特征。方法选择广州市番禺地区户籍参加献血和在医院体检人员6640例，其中 A 型血5262例，AB 型血1378例；采用120孔微量板法，使用抗－A1试剂对血样本进行抗－A1筛查；采用试管法，对抗－A1阴性样本进行血清学 A2亚型表型鉴定。采用序列特异性引物－聚合酶链反应（PCR－SSP）法（A2基因分型检测试剂盒）对抗－A1阴性样本的基因组 DNA 进行扩增，对扩增产物进行电泳分析，根据电泳图谱判断 A2基因分型类型。结果在5262例 A 型血样本中，筛查出抗－A1阴性14例，经鉴定均为 A2亚型，发生频率为0．27％（14／5262），其中男性10例，女性4例。在1378例 AB 型样本中，筛查出抗－A1阴性48例，经鉴定均为 A2B 亚型，发生频率为3．48％（48／1378），其中男性34例，女性14例。在14例 A2样本中，检出 A201基因型2例，A205基因型4例，发生频率为0．11％（6／5262）；在48例 A2B 样本中，检出 A201基因型4例，A205基因型15例，发生频率为1．38％（19／1378）。结论广州番禺区 A2基因亚型以A205为主，使用血清学结合PCR－SSP方法能准确鉴定 A2亚型。
목적：분석 A2아형빈솔분포화기인분형적특정。방법선택엄주시번우지구호적삼가헌혈화재의원체검인원6640례，기중 A 형혈5262례，AB 형혈1378례；채용120공미량판법，사용항－A1시제대혈양본진행항－A1사사；채용시관법，대항－A1음성양본진행혈청학 A2아형표형감정。채용서렬특이성인물－취합매련반응（PCR－SSP）법（A2기인분형검측시제합）대항－A1음성양본적기인조 DNA 진행확증，대확증산물진행전영분석，근거전영도보판단 A2기인분형류형。결과재5262례 A 형혈양본중，사사출항－A1음성14례，경감정균위 A2아형，발생빈솔위0．27％（14／5262），기중남성10례，녀성4례。재1378례 AB 형양본중，사사출항－A1음성48례，경감정균위 A2B 아형，발생빈솔위3．48％（48／1378），기중남성34례，녀성14례。재14례 A2양본중，검출 A201기인형2례，A205기인형4례，발생빈솔위0．11％（6／5262）；재48례 A2B 양본중，검출 A201기인형4례，A205기인형15례，발생빈솔위1．38％（19／1378）。결론엄주번우구 A2기인아형이A205위주，사용혈청학결합PCR－SSP방법능준학감정 A2아형。
Objective To analyze the frequency distribution and genotyping characteristics of A2 subgroup.Methods 6 640 cases participated in blood donation and clinical examination were enrolled in the study,including 5 262 cases of A blood group and 1 378 cases of AB blood group.Their household register was Panyu Prefecture,Guangzhou City.The 120-hole micro-plate method was applied to anti-A1 screening in blood samples with anti-A1 reagent.The test tube method was used to perform serological A2 subgroup phenotypic identification in negative anti-A1 samples.Polymerase chain reaction-sequence specific primers(PCR-SSP) with A2 genotyping assay kit was used to amplify the genome DNA of negative anti-A1 samples,and then electrophoresis was adopted to analyze the amplified products. A2 genotype was determined based on the electrophorograms.Results Fourteen negative anti-A1 cases(10 male,4 female) were screened out from 5 262 samples of A blood group,identified as A2 subgroup,the negative frequency was 0.27%(14 /5 262).Forty-eight negative anti-A1 cases(34 male,14 female) were screened out from 1 378 samples of AB blood group,identified as A2B subgroup,the negative frequency was 3.48%(48 /1 378).There were 2 A201 cases and 4 A205 cases among 14 A2 samples with the frequency of 0.11%(6/5 262). There were 4 A201 cases and 15 A205 cases among 48 A2B samples with the frequency of 1.38%(19 /1 378).Conclusion A2 subgroup is dominated by A205 in Panyu Prefecture, Guangzhou City.A2 subgroup can be accurately identified with serological method and PCR-SSP.