广西医学
廣西醫學
엄서의학
GUANGXI MEDICAL JOURNAL
2015年
6期
752-754
,共3页
软坚护肝片%免疫性肝损伤%脂质过氧化%小鼠
軟堅護肝片%免疫性肝損傷%脂質過氧化%小鼠
연견호간편%면역성간손상%지질과양화%소서
Ruanjian Hugan Tablet%Immunological hepatic injury%Lipid peroxidation%Mouse
目的：观察软坚护肝片（RJHGT）对刀豆蛋白 A（ConA）所致小鼠免疫性肝损伤的保护作用，并探讨其作用机制。方法将60只小鼠随机分为正常组、模型组、阳性对照组、RJHGTH 组、RJHGTM 组、RJHGTL 组，每组10只；正常组和模型组以0．2 ml ／10 g 生理盐水灌胃，阳性对照组给予联苯双酯0．15 g／kg 灌胃，RJHGTH 组、RJHGTM 组、RJHGTL 组以0．2 ml／10 g分别给予 RJHGT 溶液1．24、0．62、0．31 g ／kg 灌胃，1次／d，连续7 d。末次给药后4 h，正常组尾静脉注射生理盐水，其余各组尾静脉注射 ConA 制备小鼠免疫性肝损伤模型。检测各组小鼠血清肿瘤坏死因子α（TNF－α）和γ干扰素（IFN－γ）、ALT、AST 等水平；检测肝组织谷胱甘肽（GSH）及超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）等指标。结果与模型组比较，各浓度 RJHGT 组小鼠血清 ALT、AST、TNF－α、IFN－γ水平及肝组织中 MDA、NO 含量均降低，肝组织 SOD 和 GSH 水平均升高（P ＜0．05）。结论RJHGT 对免疫性肝损伤小鼠具有一定的保护作用。
目的：觀察軟堅護肝片（RJHGT）對刀豆蛋白 A（ConA）所緻小鼠免疫性肝損傷的保護作用，併探討其作用機製。方法將60隻小鼠隨機分為正常組、模型組、暘性對照組、RJHGTH 組、RJHGTM 組、RJHGTL 組，每組10隻；正常組和模型組以0．2 ml ／10 g 生理鹽水灌胃，暘性對照組給予聯苯雙酯0．15 g／kg 灌胃，RJHGTH 組、RJHGTM 組、RJHGTL 組以0．2 ml／10 g分彆給予 RJHGT 溶液1．24、0．62、0．31 g ／kg 灌胃，1次／d，連續7 d。末次給藥後4 h，正常組尾靜脈註射生理鹽水，其餘各組尾靜脈註射 ConA 製備小鼠免疫性肝損傷模型。檢測各組小鼠血清腫瘤壞死因子α（TNF－α）和γ榦擾素（IFN－γ）、ALT、AST 等水平；檢測肝組織穀胱甘肽（GSH）及超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）等指標。結果與模型組比較，各濃度 RJHGT 組小鼠血清 ALT、AST、TNF－α、IFN－γ水平及肝組織中 MDA、NO 含量均降低，肝組織 SOD 和 GSH 水平均升高（P ＜0．05）。結論RJHGT 對免疫性肝損傷小鼠具有一定的保護作用。
목적：관찰연견호간편（RJHGT）대도두단백 A（ConA）소치소서면역성간손상적보호작용，병탐토기작용궤제。방법장60지소서수궤분위정상조、모형조、양성대조조、RJHGTH 조、RJHGTM 조、RJHGTL 조，매조10지；정상조화모형조이0．2 ml ／10 g 생리염수관위，양성대조조급여련분쌍지0．15 g／kg 관위，RJHGTH 조、RJHGTM 조、RJHGTL 조이0．2 ml／10 g분별급여 RJHGT 용액1．24、0．62、0．31 g ／kg 관위，1차／d，련속7 d。말차급약후4 h，정상조미정맥주사생리염수，기여각조미정맥주사 ConA 제비소서면역성간손상모형。검측각조소서혈청종류배사인자α（TNF－α）화γ간우소（IFN－γ）、ALT、AST 등수평；검측간조직곡광감태（GSH）급초양화물기화매（SOD）、병이철（MDA）、일양화담（NO）등지표。결과여모형조비교，각농도 RJHGT 조소서혈청 ALT、AST、TNF－α、IFN－γ수평급간조직중 MDA、NO 함량균강저，간조직 SOD 화 GSH 수평균승고（P ＜0．05）。결론RJHGT 대면역성간손상소서구유일정적보호작용。
Objective To observe the protective effect of Ruanjian Hugan Tablet( RJHGT) on immunological hepatic injury induced by ConA in mice,and to explore its mechanism.Methods Sixty mice were randomly divided into normal control group,model group,positive control group,RJHGTH group,RJHGTM group and RJHGTL group,with 10 mice in each group.Normal group and model group were given normal saline(0.2 ml/10 g) by gavage,positive control group was given bifendate(0.15 g/kg) by gavage,RJHGTH group,RJHGTM group and RJHGTL group were given RJHGT solution (0.2 ml/10 g) by gavage at the dosage of 1.24,0.62 and 0.31 g/kg,respectively,once a day for seven days.Four hours after the final administration,normal group was given venacaudalis injection with normal saline,while the other groups were given venacaudalis injection with ConA to develop the mouse model of immunological hepatic injury.The levels of serum tumor necrosis factor(TNF)-α,interferon(IFN)-γ,ALT and AST of the mice were detected in each group,and the contents of glutathione(GSH),superoxide dismutase(SOD),malondialdehyde(MDA) and nitric oxide(NO) in hepatic tissues were detected as well.Results Compared with model group,the mice in each RJHGT group showed decreased levels of serum ALT,AST,TNF-αand IFN-γ,decreased contents of MDA and NO in hepatic tissues,and increased levels of SOD and GSH in hepatic tissues (P <0.05). Conclusion RJHGT has protective effects on mice with immunological hepatic injury.
