CAJ | 학술논문

目的：探讨用高脂饮食诱导载脂蛋白 E（ ApoE）基因敲除小鼠制作血管内皮功能失调模型的可行性。方法高脂饮食饲养 ApoE－／－小鼠（ApoE 组）以及野生型 C57BL ／6J 小鼠（对照组），8周后，检测两组小鼠血脂、血糖、血胰岛素及一氧化氮水平；取主动脉做病理观察及血管内皮依赖性血管舒张功能实验。结果高脂喂养8周后 ApoE 组小鼠主动脉出现明显粥样硬化斑块；ApoE 组小鼠的血清总胆固醇、HDL－C、LDL－C 水平显著高于对照组小鼠（P ＜0．05），血清一氧化氮水平显著低于对照组（P ＜0．05）；两组小鼠的血糖、血清胰岛素水平均在正常范围，且差异无统计学意义（P ＞0．05）；ApoE 组小鼠主动脉内皮依赖性舒张功能最大舒张率为（51．14±6．21）％，显著低于对照组小鼠的（86．52±2．88）％（P ＜0．05）。结论经过8周高脂饮食喂养的 ApoE 基因敲除小鼠可以作为研究内皮功能失调的理想动物模型。
목적：탐토용고지음식유도재지단백 E（ ApoE）기인고제소서제작혈관내피공능실조모형적가행성。방법고지음식사양 ApoE－／－소서（ApoE 조）이급야생형 C57BL ／6J 소서（대조조），8주후，검측량조소서혈지、혈당、혈이도소급일양화담수평；취주동맥주병리관찰급혈관내피의뢰성혈관서장공능실험。결과고지위양8주후 ApoE 조소서주동맥출현명현죽양경화반괴；ApoE 조소서적혈청총담고순、HDL－C、LDL－C 수평현저고우대조조소서（P ＜0．05），혈청일양화담수평현저저우대조조（P ＜0．05）；량조소서적혈당、혈청이도소수평균재정상범위，차차이무통계학의의（P ＞0．05）；ApoE 조소서주동맥내피의뢰성서장공능최대서장솔위（51．14±6．21）％，현저저우대조조소서적（86．52±2．88）％（P ＜0．05）。결론경과8주고지음식위양적 ApoE 기인고제소서가이작위연구내피공능실조적이상동물모형。
Objective To explore the feasibility of establishing the vascular endothelial dysfunction model induced by high cholesterol diet in apolipoprotein E(ApoE) knockout mice.Methods ApoE-/-knockout mice(ApoE group) and wild-type(WT) C57BL/6J mice(control group) were fed with high cholesterol diet for 8 weeks.And then the levels of blood lipid,blood glucose,blood insulin and nitric oxide were detected in two groups.The arterial samples were collected for the histopathological observation and endothelium-dependent vasodilatation test. Results Significant aortic atherosclerotic plaques were observed in ApoE-/-mice after 8-week high cholesterol diet.The mice in the ApoE group had significantly higher levels of serum TC,HDL-C and LDL-C as well as significantly lower serum nitric oxide level compared with the controls (P <0.05).The levels of blood lipid and serum insulin of the mice,which showed no significant difference between two groups(P >0.05),were normal.The maximum arterial endothelium-dependent vasodilatation value of ApoE group was significantly lower than that of control group ((51.14 ±6.21)% vs.(86.52 ±2.88)%,P <0.05).Conclusion ApoE knockout mice which have been fed with high cholesterol diet for 8 weeks can act as an ideal animal model for endothelial dysfunction research.