海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2015年
16期
2355-2357,2358
,共4页
胃癌%Artemin蛋白%免疫组化法%临床病理特征
胃癌%Artemin蛋白%免疫組化法%臨床病理特徵
위암%Artemin단백%면역조화법%림상병리특정
Gastric carcinoma%Artemin protein%Immunohistochemistry%Clinicopathological features
目的：探讨Artemin蛋白在胃癌组织中的表达及其与胃癌临床病理特征的关系。方法采用免疫组化法对90例胃癌患者的癌组织及30例胃癌患者的癌旁良性组织中的Artemin蛋白进行检测，统计学分析Artemin蛋白在胃癌组织中的表达，并探讨推测Artemin蛋白与胃癌患者的临床病理特征的关系。同时通过荧光定量RT-PCR法测定胃癌组织与癌旁组织中Artemin mRNA的表达差异。结果 Artemin蛋白在胃癌组织中阳性表达率为78.89%，明显高于癌旁良性组织的10.00%，差异具有统计学意义(P<0.05)，且Artemin蛋白在胃癌组织中的表达与胃癌患者的肿瘤浸润程度、淋巴结转移及临床TNM分期有密切关系(P<0.05)，而与胃癌患者的性别、年龄、肿瘤大小及分化程度无关(P>0.05)；Artemin mRNA在胃癌组织中的表达明显高于癌旁组织7.01倍，推测Artemin参与了胃癌的发生或发展的过程。结论胃癌组织中的Artemin蛋白表达与肿瘤的侵袭转移有密切关系，可作为有效的检测指标来预测分析胃癌的发生、发展及侵袭过程，具体机制有待进一步研究。
目的：探討Artemin蛋白在胃癌組織中的錶達及其與胃癌臨床病理特徵的關繫。方法採用免疫組化法對90例胃癌患者的癌組織及30例胃癌患者的癌徬良性組織中的Artemin蛋白進行檢測，統計學分析Artemin蛋白在胃癌組織中的錶達，併探討推測Artemin蛋白與胃癌患者的臨床病理特徵的關繫。同時通過熒光定量RT-PCR法測定胃癌組織與癌徬組織中Artemin mRNA的錶達差異。結果 Artemin蛋白在胃癌組織中暘性錶達率為78.89%，明顯高于癌徬良性組織的10.00%，差異具有統計學意義(P<0.05)，且Artemin蛋白在胃癌組織中的錶達與胃癌患者的腫瘤浸潤程度、淋巴結轉移及臨床TNM分期有密切關繫(P<0.05)，而與胃癌患者的性彆、年齡、腫瘤大小及分化程度無關(P>0.05)；Artemin mRNA在胃癌組織中的錶達明顯高于癌徬組織7.01倍，推測Artemin參與瞭胃癌的髮生或髮展的過程。結論胃癌組織中的Artemin蛋白錶達與腫瘤的侵襲轉移有密切關繫，可作為有效的檢測指標來預測分析胃癌的髮生、髮展及侵襲過程，具體機製有待進一步研究。
목적：탐토Artemin단백재위암조직중적표체급기여위암림상병리특정적관계。방법채용면역조화법대90례위암환자적암조직급30례위암환자적암방량성조직중적Artemin단백진행검측，통계학분석Artemin단백재위암조직중적표체，병탐토추측Artemin단백여위암환자적림상병리특정적관계。동시통과형광정량RT-PCR법측정위암조직여암방조직중Artemin mRNA적표체차이。결과 Artemin단백재위암조직중양성표체솔위78.89%，명현고우암방량성조직적10.00%，차이구유통계학의의(P<0.05)，차Artemin단백재위암조직중적표체여위암환자적종류침윤정도、림파결전이급림상TNM분기유밀절관계(P<0.05)，이여위암환자적성별、년령、종류대소급분화정도무관(P>0.05)；Artemin mRNA재위암조직중적표체명현고우암방조직7.01배，추측Artemin삼여료위암적발생혹발전적과정。결론위암조직중적Artemin단백표체여종류적침습전이유밀절관계，가작위유효적검측지표래예측분석위암적발생、발전급침습과정，구체궤제유대진일보연구。
Objective To investigate expression of Artemin protein in gastric carcinoma tissues and its rela-tionship with the clinicopathological features. Methods The expression of Artemin protein was detected by immuno-histochemistry in gastric carcinoma tissues (from 90 patients of gastric cancer, the study group) and peficancerous tis-sues (from 30 patients of gastric cancer, the control group). The relationship of Artemin protein with the clinicopatho-logical features of gastric carcinoma patients was analyzed. The expression difference of Artemin mRNA in gastric car-cinoma tissues and peficancerous tissues was also analyzed. Results The positive expression of Artemin protein in gastric carcinoma tissues was significantly higher than that in peficancerous tissues (78.89%vs 10.00%, P<0.05). The expression of Artemin protein in gastric carcinoma tissues was correlated with TNM stage, infiltration depth and lyr-moh node tastasis (P<0.05), but not correlated with age, sex, tumor size or degree of differentiation (P>0.05). The ex-pression of Artemin mRNA in gastric carcinoma tissues was 7.01 times higher than that in the peficancerous tissues, which indicates that Artemin plays a role in the development of gastric carcinoma. Conclusion The expression of Ar-temin protein in gastric carcinoma significantly correlates with tumor invasion and metastasis, which could be used as one of the indicators for the clinical evaluation of gastric cancer progression and the prognosis of patients.
