CAJ | 학술논문

目的：了解该院耐碳青霉烯类肺炎克雷伯菌的耐药机制。方法选择对亚胺培南耐药的肺炎克雷伯菌(KP)7株，采用 K-B 法对其进行药敏实验。PCR 检测18种耐药基因，分析耐亚胺培南 KP 的耐药表型及其基因型。结果7株 KP 对阿米卡星、庆大霉素、妥布霉素、复方磺胺甲噁唑较为敏感，对其他常用抗菌药物均耐药。耐药基因 KPC、ant(3′′)-Ⅰ、SHV、CTX-M、ant(2′′)-Ⅰ的阳性检出率较高。结论分离的对亚胺培南耐药的 KP 携带多种耐药基因，其耐药机制主要与 KPC 有关，在此基础上可能存在其他耐药机制。
목적：료해해원내탄청매희류폐염극뢰백균적내약궤제。방법선택대아알배남내약적폐염극뢰백균(KP)7주，채용 K-B 법대기진행약민실험。PCR 검측18충내약기인，분석내아알배남 KP 적내약표형급기기인형。결과7주 KP 대아미잡성、경대매소、타포매소、복방광알갑오서교위민감，대기타상용항균약물균내약。내약기인 KPC、ant(3′′)-Ⅰ、SHV、CTX-M、ant(2′′)-Ⅰ적양성검출솔교고。결론분리적대아알배남내약적 KP 휴대다충내약기인，기내약궤제주요여 KPC 유관，재차기출상가능존재기타내약궤제。
Objective To investigate the molecular mechanism of Klebsiella pneumoniae resistant to imipenem.Methods Anti-microbial susceptibility was evaluated by disk diffusion test,Seven imipenem-resistant strains of Klebsiella pneumoniae were select-ed.And PCR was used to amplify so as to detect eighteen resistant genes.Results Seven imipenem-resistant strains of Klebsiella pneumoniae were more sensitive to aminoglycosides and sulfamethoxazole/trimethoprim.The positive rates of KPC,ant(3′′)-Ⅰ, SHV,CTX-Mand ant(2′′)-Ⅰin these imipenem-resistant strains were high.Conclusion Carbapenem-resistant Klebsiella pneumon-iae isolated from our hospital carries several kinds of resistant genes.Carrying KPC may be the main resistant mechanism of Kleb-siella pneumoniae resistant to carbapenem,but other mechanisms also exist at the same time.