CAJ | 학술논문

目的：通过测定过敏性紫癜患者白细胞介素-8(IL-8)、免疫球蛋白 A/补体3(IgA/C3),探讨二者之间的相关性,研究过敏性紫癜患者相应的免疫失衡状况,为临床采用更有效的治疗方法提供理论依据。方法酶联免疫吸附法测定 IL-8,全自动生化分析仪测定 IgA、C3,并计算 IgA/C3。结果双抗体夹心法(ELISA)检测实验组血清中 IL-8表达量为(389.00±51.68)ng/L,与对照组相比显著升高(P <0.05)。IgA 水平为(306.2±32.21)mg/L,与对照组相比显著升高(P <0.05)。C3水平为(114.5±20.7)mg/L,与对照组比较,差异无统计学意义(P >0.05)。IgA/C3为3.0410±0.5085,与对照组相比显著升高(P <0.05)。IL-8与 IgA/C3呈正相关关系(r=0.534,P <0.05)。结论过敏性紫癜患者存在细胞因子与免疫球蛋白水平紊乱,体液免疫和细胞免疫均处在高反应状态,IL-8与 IgA/C3呈正相关关系。
목적：통과측정과민성자전환자백세포개소-8(IL-8)、면역구단백 A/보체3(IgA/C3),탐토이자지간적상관성,연구과민성자전환자상응적면역실형상황,위림상채용경유효적치료방법제공이론의거。방법매련면역흡부법측정 IL-8,전자동생화분석의측정 IgA、C3,병계산 IgA/C3。결과쌍항체협심법(ELISA)검측실험조혈청중 IL-8표체량위(389.00±51.68)ng/L,여대조조상비현저승고(P <0.05)。IgA 수평위(306.2±32.21)mg/L,여대조조상비현저승고(P <0.05)。C3수평위(114.5±20.7)mg/L,여대조조비교,차이무통계학의의(P >0.05)。IgA/C3위3.0410±0.5085,여대조조상비현저승고(P <0.05)。IL-8여 IgA/C3정정상관관계(r=0.534,P <0.05)。결론과민성자전환자존재세포인자여면역구단백수평문란,체액면역화세포면역균처재고반응상태,IL-8여 IgA/C3정정상관관계。
Objective To investigate the correlation between serum interleukin-8 (IL-8 )and immunoglobulin/complement 3A (IgA/C3)and immune imbalance in Henoch-Schonlein Purpura(HSP)in children,and provide evidence and guidance for clinical therapy of HSP.Methods Serum IL-8 was measured by Enzyme-linked immunosorbent assay(ELISA),and IgA and C3 were de-tected by automatic biochemistry analyzer,and calculate the ratio of IgA/C3.Results Serum IL-8 levels were (389±5 1.68)ng/L, significantly higher than that in healthy children (P < 0.05 ).Serum IgA levels were(306.2 ± 32.21 )mg/L,significantly higher (P <0.05).Serum C3 levels were (1 14.5 ±20.7)mg/L,of no statistical difference.IgA/C3 were (3.041 ±0.508 5),significantly higher(P <0.05).There was a positive correlation between IL-8 and IgA/C3 (r =0.534,P <0.05 ).Conclusion The children of HSP has cytokine and immunoglobulin disturbance,and presents a hyperreactivity in both humoral immunity and cellular immunity. Positive correlation between IL-8 and IgA/C3 was observed.