CAJ | 학술논문

目的：建立固相萃取及毛细管电泳法快速检测血浆和尿液中喹诺酮类药物洛美沙星、加替沙星、环丙沙星和氧氟沙星的方法。方法毛细管为熔融石英毛细管(75/365μm,40/47 cm),运行缓冲液为40 mmol/L 硼砂缓冲液(pH 9.0),电压13 kV,柱温为20℃,检测波长280 nm。样品经固相萃取后进样分析。结果4种喹诺酮类药物在6 min 内完全分离。在1~40μg/mL 浓度范围内,洛美沙星、加替沙星、环丙沙星和氧氟沙星峰面积和浓度的线性关系良好,相关系数 r 分别为0.9987、0.9976、0.9983和0.9942。尿液和血浆中4种喹诺酮类药物加标回收率为80.1%~107.6%,精密度相对标准差(RSD)为2.1%~6.2%。结论该方法具有快速、简便、准确度和精密度高等特点,适用于血浆和尿液中洛美沙星、加替沙星、环丙沙星和氧氟沙星水平的快速检测。
목적：건립고상췌취급모세관전영법쾌속검측혈장화뇨액중규낙동류약물락미사성、가체사성、배병사성화양불사성적방법。방법모세관위용융석영모세관(75/365μm,40/47 cm),운행완충액위40 mmol/L 붕사완충액(pH 9.0),전압13 kV,주온위20℃,검측파장280 nm。양품경고상췌취후진양분석。결과4충규낙동류약물재6 min 내완전분리。재1~40μg/mL 농도범위내,락미사성、가체사성、배병사성화양불사성봉면적화농도적선성관계량호,상관계수 r 분별위0.9987、0.9976、0.9983화0.9942。뇨액화혈장중4충규낙동류약물가표회수솔위80.1%~107.6%,정밀도상대표준차(RSD)위2.1%~6.2%。결론해방법구유쾌속、간편、준학도화정밀도고등특점,괄용우혈장화뇨액중락미사성、가체사성、배병사성화양불사성수평적쾌속검측。
Objective To develop a new method for the rapid determination of lomefloxacin,gatifloxacin,ciprofloxacin and oflox-acin in plasm and urine by solid phase extraction(SPE)and capillary electrophoresis.Methods The capillary was fused silica capil-lary with id/od of 75/365 μm and effective/total length of 40/47 cm.The running buffer was 40 mmol/L borate buffer at pH 9.0. Separation voltage was 13 kV.Temperature was 20 ℃.Detection wave-length was set at 280 nm.The sample was analyzed after the pretreatment of SPE.Results The analysis of lomefloxacin,gatifloxacin,ciprofloxacin and ofloxacin was completed in 6 minutes with satisfied accuracy and precision.Good linearity was found within the range of 1-40 μg/mL,and the r was 0.998 7,0.997 6, 0.998 3 and 0.994 2 respectively.The recoveries of four quinolones in plasm and urine ranged from 80.1% to 107.6%,and the rel-ative standard deviations(RSD)ranged from 2.1% to 6.2%.Conclusion This method is fast,simple,precise and it might be feasi-ble for the determination of lomefloxacin,gatifloxacin,ciprofloxacin and ofloxacin in plasm and urine samples.