中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
8期
779-783
,共5页
催乳素瘤%雌激素受体α%雌激素受体β%性别因素
催乳素瘤%雌激素受體α%雌激素受體β%性彆因素
최유소류%자격소수체α%자격소수체β%성별인소
Prolactinoma%Estrogen receptor alpha%Estrogen receptor beta%Sex factors
目的 探讨不同性别垂体催乳素(PRL)腺瘤患者雌激素受体(ER)表达的差异及其与PRL水平、肿瘤大小的关系.方法 纳入2013年3月至2014年7月中国医学科学院北京协和医院神经外科手术治疗的垂体PRL腺瘤患者26例,其中男7例,女19例.收集患者术前的PRL水平、肿瘤大小等临床资料,采用实时定量PCR法检测ERα和ERβ mRNA的表达,免疫组化法检测ERα和ERβ蛋白的表达.分析不同性别患者术前的PRL水平、肿瘤大小与ERα和ERβ mRNA表达的关系.结果 术前男、女患者PRL值的中位数(范围)分别为1 574(224 ～4 305)、270(129 ～1 000) ng/ml,肿瘤体积的中位数(范围)分别为3.496(0.150～13.13)、0.832(0.100 ～ 16.20)cm3,差异均有统计学意义(均P＜0.01).男性患者肿瘤标本中ERα和ERβ的mRNA及蛋白的表达水平均明显高于女性患者,差异有统计学意义(均P＜0.01).ERα-mRNA、ERβ-mRNA的表达水平与术前PRL水平均呈正相关(R2值分别为0.8753、0.2601,均P＜0.01),与肿瘤大小同样呈正相关(R2值分别为0.4598,0.3740,均P＜0.01).结论 垂体PRL腺瘤患者的PRL水平和肿瘤体积具有性别差异,引起差异的机制可能与ERα和ERβ通过多种途径参与PRL腺瘤的增殖有关.
目的 探討不同性彆垂體催乳素(PRL)腺瘤患者雌激素受體(ER)錶達的差異及其與PRL水平、腫瘤大小的關繫.方法 納入2013年3月至2014年7月中國醫學科學院北京協和醫院神經外科手術治療的垂體PRL腺瘤患者26例,其中男7例,女19例.收集患者術前的PRL水平、腫瘤大小等臨床資料,採用實時定量PCR法檢測ERα和ERβ mRNA的錶達,免疫組化法檢測ERα和ERβ蛋白的錶達.分析不同性彆患者術前的PRL水平、腫瘤大小與ERα和ERβ mRNA錶達的關繫.結果 術前男、女患者PRL值的中位數(範圍)分彆為1 574(224 ～4 305)、270(129 ～1 000) ng/ml,腫瘤體積的中位數(範圍)分彆為3.496(0.150～13.13)、0.832(0.100 ～ 16.20)cm3,差異均有統計學意義(均P＜0.01).男性患者腫瘤標本中ERα和ERβ的mRNA及蛋白的錶達水平均明顯高于女性患者,差異有統計學意義(均P＜0.01).ERα-mRNA、ERβ-mRNA的錶達水平與術前PRL水平均呈正相關(R2值分彆為0.8753、0.2601,均P＜0.01),與腫瘤大小同樣呈正相關(R2值分彆為0.4598,0.3740,均P＜0.01).結論 垂體PRL腺瘤患者的PRL水平和腫瘤體積具有性彆差異,引起差異的機製可能與ERα和ERβ通過多種途徑參與PRL腺瘤的增殖有關.
목적 탐토불동성별수체최유소(PRL)선류환자자격소수체(ER)표체적차이급기여PRL수평、종류대소적관계.방법 납입2013년3월지2014년7월중국의학과학원북경협화의원신경외과수술치료적수체PRL선류환자26례,기중남7례,녀19례.수집환자술전적PRL수평、종류대소등림상자료,채용실시정량PCR법검측ERα화ERβ mRNA적표체,면역조화법검측ERα화ERβ단백적표체.분석불동성별환자술전적PRL수평、종류대소여ERα화ERβ mRNA표체적관계.결과 술전남、녀환자PRL치적중위수(범위)분별위1 574(224 ～4 305)、270(129 ～1 000) ng/ml,종류체적적중위수(범위)분별위3.496(0.150～13.13)、0.832(0.100 ～ 16.20)cm3,차이균유통계학의의(균P＜0.01).남성환자종류표본중ERα화ERβ적mRNA급단백적표체수평균명현고우녀성환자,차이유통계학의의(균P＜0.01).ERα-mRNA、ERβ-mRNA적표체수평여술전PRL수평균정정상관(R2치분별위0.8753、0.2601,균P＜0.01),여종류대소동양정정상관(R2치분별위0.4598,0.3740,균P＜0.01).결론 수체PRL선류환자적PRL수평화종류체적구유성별차이,인기차이적궤제가능여ERα화ERβ통과다충도경삼여PRL선류적증식유관.
Objective To investigate the difference of estrogen receptor (ER) expression of different genders in patients with pituitary prolactin (PRL) adenoma and its relationship with PRL levels and tumor size.Methods Twenty-six patients with PRL adenoma treated surgically at the Department of Neurosurgery,Chinese Academy of Medical Sciences & Peking Union Medical College Hospital from March 2013 to July 2014 were enrolled,including 19 females and 7 males.The clinical information,including the preoperative PRL levels and tumor sizes of the patients,was collected.Real-time quantitative PCR was used to detect the expression levels of ERα and ERβ mRNA.Immunohistochemistry was used to detect the expression levels of ERα and ERβ.The relationship between the preoperative PRL levels,tumor size and the expression levels of ERα and ERβ were analyzed.Results The preoperative median PRL values of the male and female patients were 1574 (224-4305) and 270 (129-1000) ng/ml respectively;their median tumor volumes were 3.496 (0.150-13.13) and 0.832 (0.100-16.20) cm3 respectively,and there were significant differences (all P ＜0.01).The expression levels of ERo,ERβ mRNA,and protein of the male patients in the tumor samples were significantly higher than those of the female patients,and there was significant difference (all P ＜ 0.01).The expression levels of ERα mRNA,ERβ mRNA and the level of preoperative PRL had positive correlation (R2 =0.8753 and 0.2601 respectively;all P ＜ 0.01),and they also had positive correlation with the tumor size (R2 =0.4598 and 0.3740 respectively;all P ＜ 0.01).Conclusions There is a sex difference in the PRL levels and tumor volumes in patients with PRL adenoma.The mechanisms of causing difference may be associated with ERα and ERβ participating in the proliferation of PRL adenoma through a variety of approaches.
