CAJ | 학술논문

目的：调查分析深圳地区2003~2013年献血者抗-丙型肝炎病毒(HCV)抗体/病毒核酸检测(NAT)阳性检出情况。方法献血者经乙型肝炎病毒表面抗原(HBsAg)、丙氨酸氨基转移酶(ALT)快速试纸条初筛后,使用进口与国产两种酶联免疫吸附试验(ELISA)及血液病毒 NAT 方法检测抗-HCV 抗体、HCV RNA,对11年来献血者抗-HCV 抗体、NAT 阳性率流行趋势进行统计分析比较。结果深圳地区2003~2013年献血人数逐年增长,抗-HCV 抗体阳性率呈 M 型检出走势,于2013年达到最低。献血者抗-HCV 抗体阳性/NAT 阴性检出率与抗-HCV 抗体走势一致,而真实感染 HCV 的指标抗-HCV 抗体与 NAT 双阳性组检出率则缓慢下降；对抗-HCV 抗体阳性献血者在不同分类组别比较发现,31~45岁献血者组、初次献血者组的抗-HCV 抗体检出率分别高于其他年龄组与重复献血者(P <0.05)；抗-HCV 抗体阴性/NAT 阳性献血者有3例(检出率为1/134518)。结论NAT 检测为常规酶联免疫方法的补充检测手段,缺一不可,能大大降低输血风险,而 NAT 阴性献血者的抗-HCV 抗体阳性占多数的检测结果应引起重视,避免因假阳性导致此类献血者资格淘汰。
목적：조사분석심수지구2003~2013년헌혈자항-병형간염병독(HCV)항체/병독핵산검측(NAT)양성검출정황。방법헌혈자경을형간염병독표면항원(HBsAg)、병안산안기전이매(ALT)쾌속시지조초사후,사용진구여국산량충매련면역흡부시험(ELISA)급혈액병독 NAT 방법검측항-HCV 항체、HCV RNA,대11년래헌혈자항-HCV 항체、NAT 양성솔류행추세진행통계분석비교。결과심수지구2003~2013년헌혈인수축년증장,항-HCV 항체양성솔정 M 형검출주세,우2013년체도최저。헌혈자항-HCV 항체양성/NAT 음성검출솔여항-HCV 항체주세일치,이진실감염 HCV 적지표항-HCV 항체여 NAT 쌍양성조검출솔칙완만하강；대항-HCV 항체양성헌혈자재불동분류조별비교발현,31~45세헌혈자조、초차헌혈자조적항-HCV 항체검출솔분별고우기타년령조여중복헌혈자(P <0.05)；항-HCV 항체음성/NAT 양성헌혈자유3례(검출솔위1/134518)。결론NAT 검측위상규매련면역방법적보충검측수단,결일불가,능대대강저수혈풍험,이 NAT 음성헌혈자적항-HCV 항체양성점다수적검측결과응인기중시,피면인가양성도치차류헌혈자자격도태。
Objective To investigate and analyze the popular trend of HBV infection in blood donors in Shenzhen City from 2003 to 2012.Methods After preliminary routine strip screening of HBsAg、ALT,blood samples were tested by using both domestic and imported ELISA reagents along with nucleic acid detection,then tried to find a trend of HCV prevalence in blood donor by analysis data.Results Blood donors′number rised year by year from 2003 to 2013,but prevalence of anti-HCV gave a volatile reception, which had a lowest rate at 2013.The detection rate of anti-HCV positive/NAT-was basically the same as anti-HCV positive,where-as the detection rate of anti-HCV positive/NAT positive declined slightly in those confirmed HCV infectors;blood donors were di-vided into different age groups,found that most HCV positive donors were between 31 to 45 yearsold and prevalence of HCV in first-time donors was much higher than repeat donors (P <0.05);only 3 anti-HCV negative/NAT positive samples were found in this study(detection rate:1/134 5 18).Conclusion According to this study,NAT test might be a necessary auxiliary blood screen method to routine ELISA test which could reduce the risk of blood transfusion.Besides,it is necessary to pay attention to the large amount of anti-HCV positive/NAT-samples and avoid to discard safe blood samples because of false positive.