CAJ | 학술논문

目的：筛选碳青霉烯类耐药鲍曼不动杆菌并了解其耐药情况,分析碳青霉烯类耐药菌及碳青霉烯类敏感菌的外膜蛋白表达情况。方法采用琼脂二倍稀释法筛选碳青霉烯类耐药鲍曼不动杆菌,运用聚合酶链反应(PCR)技术检测外膜蛋白基因carO；十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析细菌外膜蛋白的表达情况。结果110株临床分离鲍曼不动杆菌中的32株细菌对碳青霉烯类抗菌药物耐药,此32株碳青霉烯类耐药菌除对多黏菌素 B 敏感性为100%,对其他所试药物均呈不同程度耐药；碳青霉烯类鲍曼不动杆菌均携带 carO 基因,SDS-PAGE 分析显示碳青霉烯类耐药菌株与敏感菌株相比,在相对分子质量约22×103和50×103等处的蛋白条带存在差异。结论鲍曼不动杆菌对抗菌药物的耐药情况严重,且碳青霉烯类耐药菌与敏感菌的外膜蛋白在表达上存在明显差异。
목적：사선탄청매희류내약포만불동간균병료해기내약정황,분석탄청매희류내약균급탄청매희류민감균적외막단백표체정황。방법채용경지이배희석법사선탄청매희류내약포만불동간균,운용취합매련반응(PCR)기술검측외막단백기인carO；십이완기류산납-취병희선알응효전영(SDS-PAGE)분석세균외막단백적표체정황。결과110주림상분리포만불동간균중적32주세균대탄청매희류항균약물내약,차32주탄청매희류내약균제대다점균소 B 민감성위100%,대기타소시약물균정불동정도내약；탄청매희류포만불동간균균휴대 carO 기인,SDS-PAGE 분석현시탄청매희류내약균주여민감균주상비,재상대분자질량약22×103화50×103등처적단백조대존재차이。결론포만불동간균대항균약물적내약정황엄중,차탄청매희류내약균여민감균적외막단백재표체상존재명현차이。
Objective To Screen the carbapenem-resistant acinetobacter baumannii so as to understand the drug resistance,and to analyze the outer membrane protein expression between the carbapenem-resistant Acinetobacter baumannii and the sensitive strains.Methods The twofold agar dilution method was used to screen the carbapenem-resistant acinetobacter baumannii.PCR technique was used to detect the outer membrane protein-encoding gene carO.SDS-PAGE was used to analyze the expression of outer membrane protein.Results 32 strains were carbapenem-resistant acinetobacter baumannii separated from 1 10 strains,which were 100% sensitive to polymyxin B and were resistant to other drugs with different degrees O.They all carried carO gene.There were different protein bands between the carbapenem-resistant strains and the sensitive strains,in which two protein bands of 50× 10 3 and 22×10 3 were recognized as outer membrane proteins through the analysis of SDS-PAGE.Conclusion Drug resistance of acinetobacter baumannii is serious,and the outer membrane protein expression between the carbapenem-resistant strains and the sensitive strains presents discrepancy.