华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2015年
4期
361-365
,共5页
吕雪%柳娜%杜文%李佳洋%孙玥%罗恩
呂雪%柳娜%杜文%李佳洋%孫玥%囉恩
려설%류나%두문%리가양%손모%라은
脂联素%腺病毒%骨缺损%成骨
脂聯素%腺病毒%骨缺損%成骨
지련소%선병독%골결손%성골
adiponectin%adenovirus%bone defect%osteogenesis
目的:??探讨局部应用人脂联素(hAPN)基因重组腺病毒Ad-hAPN-EGFP对SD大鼠胫骨骨缺损修复的影响。方法??采用36只SD大鼠进行胫骨骨缺损(缺损直径2?mm)修复实验,36只大鼠(72侧)随机分为A、B、C共3组(n=24),在术中及术后第2天分别注射重组腺病毒Ad-hAPN-EGFP、空白腺病毒Ad-EGFP和生理盐水。术后1周采用实时荧光定量聚合酶链反应(real-time?PCR)对hAPN及成骨相关因子的表达进行检测,术后3周采用显微CT、苏木精-伊红(HE)染色及Masson染色对骨缺损修复情况进行对比。结果??1)术后1周real-time?PCR显示,A组hAPN表达成功,而B、C组无表达;A组成骨相关因子的表达量明显高于B、C组(P<0.05)。2)术后3周显微CT、HE染色及Masson染色显示,A组新生骨密度、骨体积分数、骨小梁数量及骨小梁厚度均大于B、C组(P<0.05),而B、C两组间无明显差异(P>0.05)。结论??局部应用hAPN基因重组腺病毒对大鼠骨缺损修复具有促进作用。
目的:??探討跼部應用人脂聯素(hAPN)基因重組腺病毒Ad-hAPN-EGFP對SD大鼠脛骨骨缺損脩複的影響。方法??採用36隻SD大鼠進行脛骨骨缺損(缺損直徑2?mm)脩複實驗,36隻大鼠(72側)隨機分為A、B、C共3組(n=24),在術中及術後第2天分彆註射重組腺病毒Ad-hAPN-EGFP、空白腺病毒Ad-EGFP和生理鹽水。術後1週採用實時熒光定量聚閤酶鏈反應(real-time?PCR)對hAPN及成骨相關因子的錶達進行檢測,術後3週採用顯微CT、囌木精-伊紅(HE)染色及Masson染色對骨缺損脩複情況進行對比。結果??1)術後1週real-time?PCR顯示,A組hAPN錶達成功,而B、C組無錶達;A組成骨相關因子的錶達量明顯高于B、C組(P<0.05)。2)術後3週顯微CT、HE染色及Masson染色顯示,A組新生骨密度、骨體積分數、骨小樑數量及骨小樑厚度均大于B、C組(P<0.05),而B、C兩組間無明顯差異(P>0.05)。結論??跼部應用hAPN基因重組腺病毒對大鼠骨缺損脩複具有促進作用。
목적:??탐토국부응용인지련소(hAPN)기인중조선병독Ad-hAPN-EGFP대SD대서경골골결손수복적영향。방법??채용36지SD대서진행경골골결손(결손직경2?mm)수복실험,36지대서(72측)수궤분위A、B、C공3조(n=24),재술중급술후제2천분별주사중조선병독Ad-hAPN-EGFP、공백선병독Ad-EGFP화생리염수。술후1주채용실시형광정량취합매련반응(real-time?PCR)대hAPN급성골상관인자적표체진행검측,술후3주채용현미CT、소목정-이홍(HE)염색급Masson염색대골결손수복정황진행대비。결과??1)술후1주real-time?PCR현시,A조hAPN표체성공,이B、C조무표체;A조성골상관인자적표체량명현고우B、C조(P<0.05)。2)술후3주현미CT、HE염색급Masson염색현시,A조신생골밀도、골체적분수、골소량수량급골소량후도균대우B、C조(P<0.05),이B、C량조간무명현차이(P>0.05)。결론??국부응용hAPN기인중조선병독대대서골결손수복구유촉진작용。
Objective??To?investigate?the?effect?of?local?administration?of?recombinant?adenovirus?of?human?adiponectin?(hAPN)?Ad-hAPN-EGFP?on?tibial?defect?repair?of?SD?rats. Methods??Tibial?defect?(2?mm)?models?of?36?SD?rats?(72?sides)?were?randomly?divided?into?three?groups?(A,?B,?and?C;?n=24).?The?three?groups?were?injected?with?Ad-hAPN-EGFP,?Ad-EGFP,?and?normal?saline,?respectively,?during?operation?and?the?day?after?operation.?A?week?after?the?operation,?the?expres-sion?of?hAPN?and?osteogenesis-related?factors?were?detected?by?real-time?polymerase?chain?reaction.?Three?weeks?after?operation,?the?tibias?were?examined?by?micro-computed?tomography,?hematoxylin-eosin?staining,?and?Masson?staining?to?evaluate?the?restoration?of?bone?defects.?Results??1)?The?hAPN?expression?was?detected?in?group?A?but?not?in?groups?B?and?C.?Osteogenesis-related?factors?expression?of?group?A?was?significantly?higher?than?that?of?the?other?groups?(P<0.05).?2)?Osteogenesis?(including?bone?mineral?density,?relative?bone?volume,?trabecular?number?and?trabecular?thickness)?in?group?A?were?more?evident?than?those?in?groups?B?and?C?(P<0.05).?No?significant?differences?were?found?between?groups?B?and?C?(P>0.05).?Conclusion??Local?administration?of?recombinant?adenovirus?Ad-hAPN-EGFP?may?be?an?effective?strategy?to?improve?the?restoration?of?bone?defects?in vivo.