CAJ | 학술논문

目的：??探讨局部应用人脂联素（hAPN）基因重组腺病毒Ad-hAPN-EGFP对SD大鼠胫骨骨缺损修复的影响。方法??采用36只SD大鼠进行胫骨骨缺损（缺损直径2?mm）修复实验，36只大鼠（72侧）随机分为A、B、C共3组（n=24），在术中及术后第2天分别注射重组腺病毒Ad-hAPN-EGFP、空白腺病毒Ad-EGFP和生理盐水。术后1周采用实时荧光定量聚合酶链反应（real-time?PCR）对hAPN及成骨相关因子的表达进行检测，术后3周采用显微CT、苏木精-伊红（HE）染色及Masson染色对骨缺损修复情况进行对比。结果??1）术后1周real-time?PCR显示，A组hAPN表达成功，而B、C组无表达；A组成骨相关因子的表达量明显高于B、C组（P<0.05）。2）术后3周显微CT、HE染色及Masson染色显示，A组新生骨密度、骨体积分数、骨小梁数量及骨小梁厚度均大于B、C组（P<0.05），而B、C两组间无明显差异（P>0.05）。结论??局部应用hAPN基因重组腺病毒对大鼠骨缺损修复具有促进作用。
목적：??탐토국부응용인지련소（hAPN）기인중조선병독Ad-hAPN-EGFP대SD대서경골골결손수복적영향。방법??채용36지SD대서진행경골골결손（결손직경2?mm）수복실험，36지대서（72측）수궤분위A、B、C공3조（n=24），재술중급술후제2천분별주사중조선병독Ad-hAPN-EGFP、공백선병독Ad-EGFP화생리염수。술후1주채용실시형광정량취합매련반응（real-time?PCR）대hAPN급성골상관인자적표체진행검측，술후3주채용현미CT、소목정-이홍（HE）염색급Masson염색대골결손수복정황진행대비。결과??1）술후1주real-time?PCR현시，A조hAPN표체성공，이B、C조무표체；A조성골상관인자적표체량명현고우B、C조（P<0.05）。2）술후3주현미CT、HE염색급Masson염색현시，A조신생골밀도、골체적분수、골소량수량급골소량후도균대우B、C조（P<0.05），이B、C량조간무명현차이（P>0.05）。결론??국부응용hAPN기인중조선병독대대서골결손수복구유촉진작용。
Objective??To?investigate?the?effect?of?local?administration?of?recombinant?adenovirus?of?human?adiponectin?(hAPN)?Ad-hAPN-EGFP?on?tibial?defect?repair?of?SD?rats. Methods??Tibial?defect?(2?mm)?models?of?36?SD?rats?(72?sides)?were?randomly?divided?into?three?groups?(A,?B,?and?C;?n=24).?The?three?groups?were?injected?with?Ad-hAPN-EGFP,?Ad-EGFP,?and?normal?saline,?respectively,?during?operation?and?the?day?after?operation.?A?week?after?the?operation,?the?expres-sion?of?hAPN?and?osteogenesis-related?factors?were?detected?by?real-time?polymerase?chain?reaction.?Three?weeks?after?operation,?the?tibias?were?examined?by?micro-computed?tomography,?hematoxylin-eosin?staining,?and?Masson?staining?to?evaluate?the?restoration?of?bone?defects.?Results??1)?The?hAPN?expression?was?detected?in?group?A?but?not?in?groups?B?and?C.?Osteogenesis-related?factors?expression?of?group?A?was?significantly?higher?than?that?of?the?other?groups?(P<0.05).?2)?Osteogenesis?(including?bone?mineral?density,?relative?bone?volume,?trabecular?number?and?trabecular?thickness)?in?group?A?were?more?evident?than?those?in?groups?B?and?C?(P<0.05).?No?significant?differences?were?found?between?groups?B?and?C?(P>0.05).?Conclusion??Local?administration?of?recombinant?adenovirus?Ad-hAPN-EGFP?may?be?an?effective?strategy?to?improve?the?restoration?of?bone?defects?in vivo.