CAJ | 학술논문

目的：用4-硝基喹啉-1-氧化物（4NQO）诱导C57BL/6小鼠舌黏膜癌变。方法将85只小鼠随机分为蒸馏水对照组（n=5）、1,2丙二醇对照组（n=5）、实验组（n=75），分别给予蒸馏水、1,2丙二醇及4NQO溶液饮用。实验组小鼠再分为15笼，每2周处死一笼，对照组小鼠于28周处死。对小鼠进行称质量和大体观察及组织病理学检查。结果实验组死亡1只。实验组小鼠体质量在24周后体质量较对照组明显降低（P<0.05）。实验动物的舌、口底、上颚、颊部共出现79处肉眼可见的病变，其中舌部病变70处（88.6%）。实验组动物在不同时间点黏膜病变不同，28周时均发生高分化鳞状细胞癌。对照组未见病变。结论舌癌动物模型被成功建立。实验12~16周及20~28周可作为研究舌黏膜癌变早期和中晚期阶段的时间点。
목적：용4-초기규람-1-양화물（4NQO）유도C57BL/6소서설점막암변。방법장85지소서수궤분위증류수대조조（n=5）、1,2병이순대조조（n=5）、실험조（n=75），분별급여증류수、1,2병이순급4NQO용액음용。실험조소서재분위15롱，매2주처사일롱，대조조소서우28주처사。대소서진행칭질량화대체관찰급조직병이학검사。결과실험조사망1지。실험조소서체질량재24주후체질량교대조조명현강저（P<0.05）。실험동물적설、구저、상악、협부공출현79처육안가견적병변，기중설부병변70처（88.6%）。실험조동물재불동시간점점막병변불동，28주시균발생고분화린상세포암。대조조미견병변。결론설암동물모형피성공건립。실험12~16주급20~28주가작위연구설점막암변조기화중만기계단적시간점。
Objective This?study?aimed?to?induce?carcinogenesis?of?lingual?mucosa?in?C57BL/6?mice?by?feeding?them?4-nitroquinoline?1-oxide?(4NQO)?solution.?Methods???A?total?of?85?C57BL/6?mice?were?randomly?divided?into?distilled?water?control?group?(DD?group,?n=5),?1,2-propylene?glycol?control?group?(PG?group,?n=5),?and?experimental?group?(EP?group,?n=75).?The?mice?in?the?experimental?group?were?medially?fed?in?15?cages.?By?contrast,?the?mice?in?DD,?EP,?and?PG?groups?were?watered with distilled water, 50 mg·L-1?4NQO?solution,?and?1,2-propylene?glycol?solution.?The?mice?in?EP?group?were?executed?every?two?weeks?from?week?0,?and?the?mice?in?the?control?groups?were?sacrificed?at?the?28th?week.?The?mice?were?weighed.?Mucosal?lesions?were?measured?by?macroscopic?observation?and?histopathologic?detection.?Results???One?mouse?in?EP?group?died?of?unknown?reason.?The?weight?of?the?mice?in?EP?group?presented?weight?loss?compared?with?the?mice?in?DD?and?PG?groups?after?the?24th?week.?Seventy-nine?macroscopic?lesions?were?observed?in?the?lingual?mucosa,?oral?floor,?and?upper?palatal?and?buccal?mucosa.?A?total?of?70?macroscopic?lesions?(88.6%)?were?located?in?the?lingual?mucosa.?Mucosal?lesions?changed?from?simple?hyperplasia?to?squamous?cell?carcinomas.?Well-differentiated?squamous?cell?carcinomas?were?observed?in?all?mice?of?EP?group?by?pathological?section?at?the?28th?week.?No?lesion?was?found?in?the?mice?of?DD?and?PG?groups.?Conclusion?The?animal?model?of?lingual?squamous?cell?carcinomas?was?successfully?established.?The?periods?from?12th?to?16th?week?and?20th?to?28th?week?were?the?ideal?times?for?the?research?on?pathogenesis?of?early?and?medial-advanced?stage?during?carcinogenesis?of?squamous?cell?carcinomas.