CAJ | 학술논문

目的：??采用JPK?Nano?Wizard?3型生物原子力显微镜（AFM）对两种类型的山羊颞下颌关节盘细胞（软骨细胞样细胞和成纤维细胞样细胞）的表面形貌和生物力学特性进行研究，为颞下颌关节盘组织工程研究中种子细胞的选择提供依据。方法??单层培养原代山羊颞下颌关节盘细胞，生理状态下用AFM接触模式扫描两种类型的关节盘细胞，获得形貌图。随机选取20个软骨细胞样细胞和成纤维细胞样细胞，扫描获得细胞核区和细胞质区的力-位移曲线，通过JPK?Data?Processing软件分析两种细胞的杨氏模量和黏附力。结果??AFM下，三角形的软骨细胞样细胞的细胞核占据其大部分体积，细胞骨架结构呈树枝状排列，边缘伸出许多伪足；长梭形的成纤维细胞样细胞的细胞核明显高于细胞质区，骨架结构规则排列，边缘光滑，极少有伪足伸出；两种细胞的表面粗糙度没有明显差异。分析力-位移曲线得出：两种类型细胞的杨氏模量在细胞核区没有明显差异（P>0.05），在细胞质区略有差异（P=0.047）；但两种细胞的黏附力没有明显差异（P>0.05）。结论??两种类型的关节盘细胞在形貌上虽有较大的差别，但在生物力学特性上的差异并不明显。
목적：??채용JPK?Nano?Wizard?3형생물원자력현미경（AFM）대량충류형적산양섭하합관절반세포（연골세포양세포화성섬유세포양세포）적표면형모화생물역학특성진행연구，위섭하합관절반조직공정연구중충자세포적선택제공의거。방법??단층배양원대산양섭하합관절반세포，생리상태하용AFM접촉모식소묘량충류형적관절반세포，획득형모도。수궤선취20개연골세포양세포화성섬유세포양세포，소묘획득세포핵구화세포질구적력-위이곡선，통과JPK?Data?Processing연건분석량충세포적양씨모량화점부력。결과??AFM하，삼각형적연골세포양세포적세포핵점거기대부분체적，세포골가결구정수지상배렬，변연신출허다위족；장사형적성섬유세포양세포적세포핵명현고우세포질구，골가결구규칙배렬，변연광활，겁소유위족신출；량충세포적표면조조도몰유명현차이。분석력-위이곡선득출：량충류형세포적양씨모량재세포핵구몰유명현차이（P>0.05），재세포질구략유차이（P=0.047）；단량충세포적점부력몰유명현차이（P>0.05）。결론??량충류형적관절반세포재형모상수유교대적차별，단재생물역학특성상적차이병불명현。
Objective??This?study?is?performed?to?investigate?the?cell?topographies?and?biomechanical?properties?of?two?different?types?of?temporomandibular?joint?(TMJ)?discs?from?goats?by?using?JPK?Nano?Wizard?3?biological?atomic?force?microscopy?(AFM).?This?process?provides?a?guideline?for?selecting?seed?cells?for?TMJ?disc?tissue?engineering. Methods??TMJ?disc?cells?from?primary?goats?were?cultured?by?monolayer?culture?method.?AFM?was?used?to?contact?scan?the?topographies?of?the?two?types?of?TMJ?disc?cells?under?physiological?environment.?Approximately?20?chondrocyte-like?and?fibroblast-like?cells?were?selected?randomly?to?plot?the?force-versus-distance?curves?of?the?cytoplasm?and?nucleus.?Young’s?modulus?and?adhesion?were?analyzed?by?JPK?Data?Processing.?Results??The?triangle-shapednucleus?of?the?chondrocyte-like?cell?occupied?a?large?portion?of?the?cell.?Cytoskeleton?was?arranged?dendritically?on?the?surface.?Pseudopodia?were?extended?from?cell?edges.?The?spindle-shapednucleus?of?the?fibroblast-like?cell?occupied?a?significantly?larger?region?compared?with?the?cytoplasmic?region.?Cytoskeleton?was?arranged?regularly.?Cell?edges?were?smooth?with?less?pseudopodia?extended.?No?difference?was?found?in?the?surface?roughness?between?the?two?types?of?cells.?According?to?the?force-versus-distance?curves,?the?Young’s?moduli?of?the?two?types?of?cells?were?not?statistically?different?(P>0.05),?but?differences?were?found?in?the?cytoplasmic?regions?(P=0.047).?No?statistical?difference?was?found?in?the?adhesions?between?the?two?types?of?cells?(P>0.05).?Conclusion??The?AFM?topography?and?curves?were?compared?and?analyzed.?The?two?types?of?TMJ?disc?cells?exhibited?significantly?different?topographies,?but?only?slight?difference?in?their?mechanical?abilities.