CAJ | 학술논문

目的：研究伊犁黑蜂蜂胶对口腔常见致龋细菌生物膜的影响，探讨其防龋效果及可能的防龋机制。方法通过结晶紫染色法测定黑蜂蜂胶对口腔常见致龋菌（变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌）的最小生物膜清除浓度（MBEC）；培养测试细菌24?h单菌生物膜，加入MBEC及以下的3个浓度配置成初始pH值为7.0的含药培养基，厌氧培养24?h后测pH值，并计算pH变化值以检测不同浓度黑蜂蜂胶对测试菌单菌生物膜产酸能力的影响。蒽酮法测定MBEC及以下的3个浓度的黑蜂蜂胶对变异链球菌24?h单菌生物膜产生水不溶性胞外多糖的影响。结果黑蜂蜂胶对变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌的MBEC分别是6.25、1.56、3.13、0.78、0.78 mg·mL-1；黑蜂蜂胶可使各测试菌单菌生物膜ΔpH降低，蜂胶各组与对照组相比差异均有统计学意义（P<0.05）；在MBEC浓度时，蜂胶可使变异链球菌合成水不溶性胞外多糖的能力降低。结论伊犁黑蜂蜂胶具有一定的防龋效果，其可能的防龋机制是通过有效清除口腔主要致龋细菌单菌生物膜，抑制测试菌株产酸、合成水不溶性胞外多糖的能力起作用的。
목적：연구이리흑봉봉효대구강상견치우세균생물막적영향，탐토기방우효과급가능적방우궤제。방법통과결정자염색법측정흑봉봉효대구강상견치우균（변이련구균、표형련구균、혈련구균、점성방선균、내씨방선균）적최소생물막청제농도（MBEC）；배양측시세균24?h단균생물막，가입MBEC급이하적3개농도배치성초시pH치위7.0적함약배양기，염양배양24?h후측pH치，병계산pH변화치이검측불동농도흑봉봉효대측시균단균생물막산산능력적영향。은동법측정MBEC급이하적3개농도적흑봉봉효대변이련구균24?h단균생물막산생수불용성포외다당적영향。결과흑봉봉효대변이련구균、표형련구균、혈련구균、점성방선균、내씨방선균적MBEC분별시6.25、1.56、3.13、0.78、0.78 mg·mL-1；흑봉봉효가사각측시균단균생물막ΔpH강저，봉효각조여대조조상비차이균유통계학의의（P<0.05）；재MBEC농도시，봉효가사변이련구균합성수불용성포외다당적능력강저。결론이리흑봉봉효구유일정적방우효과，기가능적방우궤제시통과유효청제구강주요치우세균단균생물막，억제측시균주산산、합성수불용성포외다당적능력기작용적。
Objective To?evaluate?the?effects?of?Yili?dark?bee?propolis?on?the?main?cariogenic?biofilm?and?mechanisms.?Methods???Susceptibilities?to?the?ethanolic?extract?of?propolis?against?Streptococcus mutans?(S. mutans),?Streptococcus sobrinus?(S. sobrinus),?Streptococcus sanguis?(S. sanguis),?Actinomyces viscosus?(A. viscosus),?and?Actinomyces naeslundii (A. naeslundii)?were?analyzed?by?crystal?violet?stain?method?to?determine?the?minimum?biofilm?eradication?concentration?(MBEC).?The?biofilm?was?initially?cultivated?for?24?h.?Subsequently,?the?propolis?groups?with?different?concentration?MBEC?and?initial?pH?7.0?were?cultured?for?24?h.?Moreover,?the?pH?value?was?measured?to?evaluate?the?acid-producing?ability?of?the?tested?plaque?biofilm.?The?effects?of?propolis?on?the?insoluble?extracellular?polysaccharide?synthesis?of?S. mutans?biofilm?were?evaluated?by?anthrone?method.?Results???The?MBEC?of?Yili?propolis?on?S. mutans,?S. sobrinus,?S. sanguis,?A. viscosus,?and?A. naeslundii?were?6.25,?1.56, 3.13, 0.78, and 0.78 mg·mL-1, respectively. Propolis could decrease theΔpH of the tested plaque biofilm, and the diffe-rences?between?the?control?and?propolis?groups?were?statistically?significant?(P<0.05).?At?MBEC,?propolis?could?reduce?the?ability?of?S. mutans?in?synthesizing?insoluble?extracellular?polysaccharides.?Conclusion???Yili?propolis?demonstrate?remarkable?eradicative?effects?on?the?cariogenic?plaque?biofilm,?showing?inhibition?of?the?synthesis?of?biofilm-produced?acids?and?inso-luble?extracellular?polysaccharides.