CAJ | 학술논문

目的：??探讨程序性死亡配体1（PD-L1）在慢性牙周炎中是否有表达，以及与不同程度慢性牙周炎的相关关系，为阐明慢性牙周炎的免疫调控机制、临床治疗和预后提供依据。方法??收集健康人和慢性牙周炎患者的牙龈和牙周膜组织。根据临床探测，分为正常对照组、轻度牙周炎组、重度牙周炎组。运用荧光定量聚合酶链反应（PCR）测定不同组牙周组织中PD-L1?mRNA的表达；免疫印迹（Western?blot）以及免疫组织化学方法测定不同组牙周组织中PD-L1蛋白的表达。结合临床影像资料，分析PD-L1的差异表达量和不同程度慢性牙周炎的关系。结果??轻度牙周炎组牙周组织中PD-L1的相对表达量显著高于重度牙周炎组（P<0.01）；正常对照组牙周组织中PD-L1的相对表达量与重度牙周炎组牙周组织相比无统计学意义（P>0.05）。结论??PD-L1在牙周组织的表达可负性调控炎症牙周组织损伤。
목적：??탐토정서성사망배체1（PD-L1）재만성아주염중시부유표체，이급여불동정도만성아주염적상관관계，위천명만성아주염적면역조공궤제、림상치료화예후제공의거。방법??수집건강인화만성아주염환자적아간화아주막조직。근거림상탐측，분위정상대조조、경도아주염조、중도아주염조。운용형광정량취합매련반응（PCR）측정불동조아주조직중PD-L1?mRNA적표체；면역인적（Western?blot）이급면역조직화학방법측정불동조아주조직중PD-L1단백적표체。결합림상영상자료，분석PD-L1적차이표체량화불동정도만성아주염적관계。결과??경도아주염조아주조직중PD-L1적상대표체량현저고우중도아주염조（P<0.01）；정상대조조아주조직중PD-L1적상대표체량여중도아주염조아주조직상비무통계학의의（P>0.05）。결론??PD-L1재아주조직적표체가부성조공염증아주조직손상。
Objective??To?investigate?whether?programmed?death?ligand?1?(PD-L1)?expressed?in?the?periodontal?tissue?of?chronic?periodontitis?and?the?correlativity?of?PD-L1?and?different?degrees?of?chronic?periodontitis,?provide?experience?for?immunoregulation?mechanism,?clinical?treatment?and?prognosis?of?chronic?periodontitis. Methods??Gingiva?and?periodontal?tissue?of?healthy?people?and?chronic?periodontitis?patients?were?collected.?Based?on?clinical?probing,?periodontal?tissue?were?classified?into?three?groups:?periodontal?tissues?of?healthy?people,?periodontal?tissue?of?mild?chronic?periodontitis,?periodontal?tissue?of?severe?chronic?periodontitis.?Fluorescent?quantitation?polymerase?chain?reaction?was?applied?to?explore?the?expression?of?PD-L1?mRNA?in?the?periodontal?tissue?of?the?different?groups.?Western?blot?and?immunohistochemistry?method?were?utilized?to?test?the?expression?of?PD-L1?protein?in?the?periodontal?tissue?of?the?different?groups.?Combining?with?clinical?image?data,?the?relationship?between?differentially?expressions?of?PD-L1?and?different?degrees?of?chronic?periodontitis?was?analyzed.?Results??The?relative?expression?quantity?of?PD-L1?in?the?periodontal?tissue?of?the?mild?chronic?periodontitis?was?significantly?higher?that?of?the?severe?chronic?periodontitis?(P<0.01).?The?relative?expression?quantity?of?PD-L1?in?the?periodontal?tissue?of?healthy?subjects?and?severe?chronic?periodontitis?had?no?statistical?significance?(P>0.05).?Conclusion??The?expression?of?PD-L1?in?the?periodontal?tissue?negatively?regulates?inflammatory?periodontal?tissue?damage.