甘蔗糖业
甘蔗糖業
감자당업
SUGARCANE AND CANESUGAR
2015年
4期
58-64
,共7页
黎志德%常国炜%黄曾慰%曾练强%梁达奉
黎誌德%常國煒%黃曾慰%曾練彊%樑達奉
려지덕%상국위%황증위%증련강%량체봉
右旋糖酐酶%大孔型%离子交换树脂
右鏇糖酐酶%大孔型%離子交換樹脂
우선당항매%대공형%리자교환수지
Dextranase%Macroporous%Ion exchange resin
本试验使用大孔型离子交换树脂对来源于重组毕赤酵母的右旋糖酐酶进行分离纯化，结果表明：D151离子交换树脂能有效地吸附右旋糖酐酶，静态吸附4 h后，酶的吸附率达到53%，24 h后，吸附率达到95%；动态洗脱实验离子交换树脂可以分离酶蛋白和杂蛋白，酶的比活力提高2.43倍，但酶的回收率较低，只有8%。
本試驗使用大孔型離子交換樹脂對來源于重組畢赤酵母的右鏇糖酐酶進行分離純化，結果錶明：D151離子交換樹脂能有效地吸附右鏇糖酐酶，靜態吸附4 h後，酶的吸附率達到53%，24 h後，吸附率達到95%；動態洗脫實驗離子交換樹脂可以分離酶蛋白和雜蛋白，酶的比活力提高2.43倍，但酶的迴收率較低，隻有8%。
본시험사용대공형리자교환수지대래원우중조필적효모적우선당항매진행분리순화，결과표명：D151리자교환수지능유효지흡부우선당항매，정태흡부4 h후，매적흡부솔체도53%，24 h후，흡부솔체도95%；동태세탈실험리자교환수지가이분리매단백화잡단백，매적비활력제고2.43배，단매적회수솔교저，지유8%。
Dextranase from recombinantPichia pastoris was separated and purified by macroporous ion exchange resin. Results show that dextranase can be absorbed by D151 effectively. Dextranase absorption rate is 53.51% after four hours of static adsorption, it will be 95% after twenty-four hours. D151 is able to separate dextranase and hybridprotein. Specific activity of dextranase is 2.43 times after dynamic elution purification, but the recovery rate of dextranase is only 8%.
