CAJ | 학술논문

目的：制备鼠抗人B7-H4单克隆抗体（mAb），并对其生物学功能进行初步研究。方法以表达B7-H4分子的转基因细胞L929/B7-H4为免疫原，免疫Balb/c小鼠，采用杂交瘤技术，融合小鼠骨髓瘤细胞与脾脏细胞，用流式细胞术筛选阳性克隆，获得稳定分泌抗B7-H4 mAb的杂交瘤细胞株，并对mAb的生物学功能进行初步研究。结果成功获得1株稳定分泌抗人B7-H4 mAb的杂交瘤细胞株，命名为8F10。Western blot结果显示：8F10特异性识别B7-H4蛋白；T细胞增殖抑制阻断实验显示8F10可以阻断B7-H4对T细胞增殖的抑制作用。结论成功获得鼠抗人B7-H4 mAb，为进一步研究B7-H4分子的生物学功能提供了有效的工具。
목적：제비서항인B7-H4단극륭항체（mAb），병대기생물학공능진행초보연구。방법이표체B7-H4분자적전기인세포L929/B7-H4위면역원，면역Balb/c소서，채용잡교류기술，융합소서골수류세포여비장세포，용류식세포술사선양성극륭，획득은정분비항B7-H4 mAb적잡교류세포주，병대mAb적생물학공능진행초보연구。결과성공획득1주은정분비항인B7-H4 mAb적잡교류세포주，명명위8F10。Western blot결과현시：8F10특이성식별B7-H4단백；T세포증식억제조단실험현시8F10가이조단B7-H4대T세포증식적억제작용。결론성공획득서항인B7-H4 mAb，위진일보연구B7-H4분자적생물학공능제공료유효적공구。
ObjectiveTo prepare anti-human B7-H4 monoclonal antibody (mAb) and study its biological functions.Methods The L929/B7-H4 transfectants were used as immunogen to immunize Balb/c mice. After the fusion of murine myeloma cells with spleen B cells of the mice through hybrid technology, FCM was employed to screen positive clones and to obtain hybridoma cell line stably secreting mAb against human B7-H4, of which biological functions were studied.Results One hybridoma cell line stably secreting specific anti-human B7-H4 was established, named 8F10. Western blot result showed that 8F10 could specifi cally bind to human B7-H4 protein. The results of blocking T cell proliferation inhibition displayed that mAb 8F10 could block the inhibitory effects of B7-H4 on T cell proliferation.Conclusion One hybridoma cell line secreting anti-B7-H4 mAb was obtained. This mAb provides useful tool for further studies of B7-H4 biological function.