中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1779-1782
,共4页
覃建颖%曾建业%叶宁%段敏超%陈铭伍%郑晓宇
覃建穎%曾建業%葉寧%段敏超%陳銘伍%鄭曉宇
담건영%증건업%협저%단민초%진명오%정효우
T淋巴细胞%白细胞介素%非小细胞肺癌
T淋巴細胞%白細胞介素%非小細胞肺癌
T림파세포%백세포개소%비소세포폐암
T lymphocyte%Interleukin%Non-small-cell lung carcinoma
目的 观察白细胞介素-21(IL-21)+、CD8+、γ-干扰素+T细胞(Tc1)在非小细胞肺癌(NSCLC)患者外周血、肺癌组织及癌旁组织中的变化,探讨其在NSCLC中的作用及相互关系.方法 收集NSCLC患者外周血、癌组织及癌旁组织50例.同期收集健康对照组外周血30例.酶联免疫吸附试验(ELISA)法检测血清、癌组织及癌旁组织中IL-21的浓度;流式细胞术检测上述标本中CD8+ IL-21R+和Tc1细胞比例;磁珠分选纯化患者外周血CD8+T细胞,重组人白细胞介素-21(rhIL-21)与纯化的CD8+T细胞分别共培养24、48h,设对照组和共培养组,流式细胞术检测其Tc1细胞比例.结果 NSCLC血清中IL-21浓度高于对照组[(23.13±3.92) ng/L比(3.46±1.19) ng/L,P＜0.05],外周血CD8+ IL-21R+和Tc1细胞比例均低于对照组[(1.56±0.35)％比(3.11±1.00)％,(11.34±2.62)％比(33.94±8.38)％,P＜0.05].NSCLC癌组织中IL-21浓度高于癌旁组织[(37.82±7.24) ng/L比(15.87 ±3.82) ng/L,P＜0.05],CD8+ IL-21R+、Tc1细胞比例均高于癌旁组织[(1.62±0.25)％比(1.18±0.24)％,(5.70±1.10)％比(3.85±0.52)％,P＜0.05].IL-21浓度和CD8+ IL-21R+、Tc1细胞比例在不同TNM分期外周血、癌组织中有差异,随临床分期的进展有下降的趋势.rhIL-21与患者外周血CD8+T细胞在培养24h和48h后,培养组中Tc1细胞比例均高于对照组[(18.89±3.48)％比(13.03±2.22)％,(24.62±3.78)％比(15.14±2.76)％,P ＜0.05].结论 在NSCLC外周血及肺癌组织中,IL-21浓度升高,并随临床分期的进展而下调;CD8+IL-21R+及Tc1细胞在患者外周血中下降,而在肺癌组织处聚集,并随临床分期的进展而下调;IL-21可能通过与CD8+ IL-21R结合,刺激和活化Tc1的产生,促进γ-干扰素(IFN-γ)生成,杀伤肿瘤细胞.
目的 觀察白細胞介素-21(IL-21)+、CD8+、γ-榦擾素+T細胞(Tc1)在非小細胞肺癌(NSCLC)患者外週血、肺癌組織及癌徬組織中的變化,探討其在NSCLC中的作用及相互關繫.方法 收集NSCLC患者外週血、癌組織及癌徬組織50例.同期收集健康對照組外週血30例.酶聯免疫吸附試驗(ELISA)法檢測血清、癌組織及癌徬組織中IL-21的濃度;流式細胞術檢測上述標本中CD8+ IL-21R+和Tc1細胞比例;磁珠分選純化患者外週血CD8+T細胞,重組人白細胞介素-21(rhIL-21)與純化的CD8+T細胞分彆共培養24、48h,設對照組和共培養組,流式細胞術檢測其Tc1細胞比例.結果 NSCLC血清中IL-21濃度高于對照組[(23.13±3.92) ng/L比(3.46±1.19) ng/L,P＜0.05],外週血CD8+ IL-21R+和Tc1細胞比例均低于對照組[(1.56±0.35)％比(3.11±1.00)％,(11.34±2.62)％比(33.94±8.38)％,P＜0.05].NSCLC癌組織中IL-21濃度高于癌徬組織[(37.82±7.24) ng/L比(15.87 ±3.82) ng/L,P＜0.05],CD8+ IL-21R+、Tc1細胞比例均高于癌徬組織[(1.62±0.25)％比(1.18±0.24)％,(5.70±1.10)％比(3.85±0.52)％,P＜0.05].IL-21濃度和CD8+ IL-21R+、Tc1細胞比例在不同TNM分期外週血、癌組織中有差異,隨臨床分期的進展有下降的趨勢.rhIL-21與患者外週血CD8+T細胞在培養24h和48h後,培養組中Tc1細胞比例均高于對照組[(18.89±3.48)％比(13.03±2.22)％,(24.62±3.78)％比(15.14±2.76)％,P ＜0.05].結論 在NSCLC外週血及肺癌組織中,IL-21濃度升高,併隨臨床分期的進展而下調;CD8+IL-21R+及Tc1細胞在患者外週血中下降,而在肺癌組織處聚集,併隨臨床分期的進展而下調;IL-21可能通過與CD8+ IL-21R結閤,刺激和活化Tc1的產生,促進γ-榦擾素(IFN-γ)生成,殺傷腫瘤細胞.
목적 관찰백세포개소-21(IL-21)+、CD8+、γ-간우소+T세포(Tc1)재비소세포폐암(NSCLC)환자외주혈、폐암조직급암방조직중적변화,탐토기재NSCLC중적작용급상호관계.방법 수집NSCLC환자외주혈、암조직급암방조직50례.동기수집건강대조조외주혈30례.매련면역흡부시험(ELISA)법검측혈청、암조직급암방조직중IL-21적농도;류식세포술검측상술표본중CD8+ IL-21R+화Tc1세포비례;자주분선순화환자외주혈CD8+T세포,중조인백세포개소-21(rhIL-21)여순화적CD8+T세포분별공배양24、48h,설대조조화공배양조,류식세포술검측기Tc1세포비례.결과 NSCLC혈청중IL-21농도고우대조조[(23.13±3.92) ng/L비(3.46±1.19) ng/L,P＜0.05],외주혈CD8+ IL-21R+화Tc1세포비례균저우대조조[(1.56±0.35)％비(3.11±1.00)％,(11.34±2.62)％비(33.94±8.38)％,P＜0.05].NSCLC암조직중IL-21농도고우암방조직[(37.82±7.24) ng/L비(15.87 ±3.82) ng/L,P＜0.05],CD8+ IL-21R+、Tc1세포비례균고우암방조직[(1.62±0.25)％비(1.18±0.24)％,(5.70±1.10)％비(3.85±0.52)％,P＜0.05].IL-21농도화CD8+ IL-21R+、Tc1세포비례재불동TNM분기외주혈、암조직중유차이,수림상분기적진전유하강적추세.rhIL-21여환자외주혈CD8+T세포재배양24h화48h후,배양조중Tc1세포비례균고우대조조[(18.89±3.48)％비(13.03±2.22)％,(24.62±3.78)％비(15.14±2.76)％,P ＜0.05].결론 재NSCLC외주혈급폐암조직중,IL-21농도승고,병수림상분기적진전이하조;CD8+IL-21R+급Tc1세포재환자외주혈중하강,이재폐암조직처취집,병수림상분기적진전이하조;IL-21가능통과여CD8+ IL-21R결합,자격화활화Tc1적산생,촉진γ-간우소(IFN-γ)생성,살상종류세포.
Objective To explore the change and interaction of interleukin-21 (IL-21) + and CD8 + interferon (IFN)-γ+T (Tc1) cell in perigheral blood (PB) and lung lesion of the non small cell lung cancer (NSCLC) patients.Methods The samples of PB,lung lesion and para-cancer tissues was collected from 50 NSCLC inpatients,and the PB of 30 healthy people was control group (CG).The concentration of IL-21 in this samples was tested by enzyme linked immunosorbent assay (ELISA) kit and the proportion of CD8 + IL-21R+ and Tc1 cell was determined by flow cytometry;CD8 +T cells were obtained from PB of the patients using human T-cell isolation kit,purified CD8 + T cells were cultured with recombinant human interleukin 21 (rhIL-21) or without,co-cultured group and control group.After 24 h and 48 h of culture,the percentage of Tc1 cell was calculated by flow cytometry.Results The concentration of IL-21 in the serum from patients was significantly higher as compared to the CG [(23.13 ± 3.92) ng/L vs.(3.46 ± 1.19) ng/L (P ＜0.05)],the proportion of CD8 + IL-21R+ and Tc1 cell from patients PB was significantly lower compared with the CG,respectively [(1.56 ± 0.35) ％ vs.(3.11 ± 1.00)％ (P＜0.05),(11.34±2.62)％ vs.(33.94±8.38)％ (P＜0.05)].As well as the concentration of IL-21 from tissue homogenates [(37.82 ±7.24) ng/L vs.(15.87 ±3.82) ng/L (P＜ 0.05)],the percentage of CD8 + IL-21R + and Tc1 cell from lesion was significantly increase compared with the para-lung cancer tissues,respectively [(1.62 ± 0.25) ％ vs.(1.18 ± 0.24) ％ (P ＜ 0.05),(5.70 ± 1.10)％ vs.(3.85 ±0.52)％ (P＜0.05)].The concentration of IL-21 in serum and lesion from patients,and the frequency of Tc1,CD8 + IL-21R + cell in patients PB and lung cancer tissues both had the differentiation in different TNM phase,and had the downtrend with the development of the disease.After 24 h and 48h of culture with rhIL-21,the frequencies of Tc1 from co-cultured group were both higher than control group [(18.89±3.48)％ vs.(13.03 ±2.22)％ (P＜0.05),(24.62 ±3.78)％ vs.(15.14 ± 2.76) ％ (P ＜ 0.05)].Conclusion In the PB and lung lesion of NSCLC,the content of IL-21 was increasing and downtrend with the developing of the disease;the CD8 + IL-21R + and Tc1 cell in PB was down regulation and recruitment into lung lesion,and both had downtrend with the advance of the disease;IL-21 may combine to CD8 + IL-21 R,then promote CD8 +T cell secret the IFN-γand implement the anti-tumor effect.
