CAJ | 학술논문

目的观察食管鳞状细胞癌(ESCC)患者癌组织中程序性死亡受体-1(PD-1)表达,探讨其与患者预后关系.方法 225例ESCC组织蜡块分组为短期生存(SS)、超长期生存(ULS)、癌旁对照组织芯片.免疫组织化学(IHC)法检测组织中PD-1表达;采用t检验、方差分析确定差异显著性;Kaplan-Meier分析、Cox多因素回归模型分析PD-1蛋白表达与患者预后关系.结果不同组别患者PD-1蛋白表达比较差异有统计学意义[PD-1SS组=45,PD-1ULS组=27,PD-1对照组=10,x2=61.307,P＜0.05],且与肿瘤浸润深度(x2 =9.442,P＜0.01)、淋巴结有无转移(x2=8.599,P＜0.05)有关;生存分析显示PD-1阴性表达者预后好于阳性表达者(PD-1(-) =41,PD-1(+) =47,PD-1(++)=25,x2=14.155,P＜0.01);但其不是患者预后独立影响因素[相对危险度(RR)=1.44;95％可信区间(CI):1.01～2.06;P ＞0.05].结论 PD-1蛋白表达与ESCC患者肿瘤浸润深度、淋巴结有无转移及预后有关.
목적 관찰식관린상세포암(ESCC)환자암조직중정서성사망수체-1(PD-1)표체,탐토기여환자예후관계.방법 225례ESCC조직사괴분조위단기생존(SS)、초장기생존(ULS)、암방대조조직심편.면역조직화학(IHC)법검측조직중PD-1표체;채용t검험、방차분석학정차이현저성;Kaplan-Meier분석、Cox다인소회귀모형분석PD-1단백표체여환자예후관계.결과 불동조별환자PD-1단백표체비교차이유통계학의의[PD-1SS조=45,PD-1ULS조=27,PD-1대조조=10,x2=61.307,P＜0.05],차여종류침윤심도(x2 =9.442,P＜0.01)、림파결유무전이(x2=8.599,P＜0.05)유관;생존분석현시PD-1음성표체자예후호우양성표체자(PD-1(-) =41,PD-1(+) =47,PD-1(++)=25,x2=14.155,P＜0.01);단기불시환자예후독립영향인소[상대위험도(RR)=1.44;95％가신구간(CI):1.01～2.06;P ＞0.05].결론 PD-1단백표체여ESCC환자종류침윤심도、림파결유무전이급예후유관.
Objective To determine programmed death 1 (PD-1) expression on tumor tissues correlates with prognosis in esophageal squamous cell carcinoma (ESCC) patients.Methods Immunohistochemisty (IHC) was performed to detect the expression of PD-1 in ESCC tumor tissues and health controls.The chi-square test was applied for comparisons among different groups.Kaplan-Meier (K-M) analysis and Cox multivariate survival analysis assessed the associations of PD-1 expression with ESCC patient prognosis.Results PD-1 expressions in shorter survival (SS) group,ultra-longer survival (ULS) group and control group had a significantly difference [PD-1 SS group =45,PD-1 ULS group =27,PD-1 control group =10,x2 =61.307,P ＜ 0.05].The presence of PD-1 was related with tumor invasion (x2 =9.442,P ＜ 0.01) and lymph node metastasis (x2 =8.599,P ＜ 0.05).K-M survival analysis showed that patients for PD-1 negative expression was longer survival than those of positive ultra-positire expression [PD-1(negative) =41,PD-1 (positive) =47,PD-1(ultra-positive) =25,x2 =14.155,P＜0.01].Multivariate analysis demonstrated that PD-1 was not an independent prognostic factor for ESCC patients survival [relative risk (RR) =1.44;95％ confidence interval (CI):1.01-2.06;P ＞ 0.05].Conclusion The expression of PD-1 was correlated with tumor invasion and lymph node metastasis,the worst survival with the best positively expression.