中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1826-1830
,共5页
刘波%黄晓帆%李冬寒%王生伟%杜心灵
劉波%黃曉帆%李鼕寒%王生偉%杜心靈
류파%황효범%리동한%왕생위%두심령
环磷酸腺苷%结缔组织生长因子%心肌梗死%心肌纤维化
環燐痠腺苷%結締組織生長因子%心肌梗死%心肌纖維化
배린산선감%결체조직생장인자%심기경사%심기섬유화
Cyclic adenosine monophosphate%Connective tissue growth factor%Myocardial infarction%Myocardial fibrosis
目的 观察环磷酸腺苷(cAMP)对小鼠心肌梗死后结缔组织生长因子(CTGF)的调控作用以及对心肌纤维化的影响.方法 72只C57BL雄性小鼠,随机分为假手术组(MS组)、心肌梗死组(MI组)及药物干预组(MT组),每组各24只.术后1周MT组腹腔注射环磷酸腺苷葡胺(MAC),连续给药4周;MS组及MI组注射等量生理盐水.各组随机抽取10只小鼠行心脏超声检测及组织病理形态学检测;每组剩余小鼠按照5、5、4只随机分成3组,分离左心室梗死区及非梗死区,分别行羟脯氨酸含量测定,CTGF蛋白表达采用Western blot检测及CTGF mRNA、转化生长因子-β1(TGF-β1) mRNA表达采用实时定量聚合酶链反应(Real-time PCR)检测.结果 MT组射血分数(EF)为(19.83 ±3.95)％,左室短轴缩短率(FS)为(9.07±1.90)％,均高于MI组[EF:(13.65±2.14)％,FS:(6.22±1.03)％,P＜0.05];左心室每搏输出量(SV) MT组[(26.26±3.57)μl]优于MI组[(18.82±2.71)μl,P＜0.05].Masson染色提示MT组胶原纤维向非梗死区心肌浸润减少,免疫组织化学CTGF阳性表达较MI组减少.MT组梗死区羟脯氨酸含量[(1 388.98±86.26) μg/mg]低于MI组[(1 673.12±199.97) μg/mg,P＜0.05];非梗死区羟脯氨酸含量[(662.92 ±98.05) μg/mg]低于MI组[(855.84±83.30)μg/mg,P＜0.05].Western blot检测结果提示MT组CTGF蛋白表达量低于MI组(P＜0.05).Real-time PCR检测提示MT组CTGF mRNA表达量低于MI组(P＜0.05);TGF-β1 mRNA表达量在梗死区及非梗死区较MI组差异无统计学意义(P＞0.05).结论 环磷酸腺苷可以抑制CTGF表达,降低小鼠心肌梗死后胶原纤维的表达,减轻梗死区胶原纤维向非梗死区心肌的浸润程度,改善心脏功能.
目的 觀察環燐痠腺苷(cAMP)對小鼠心肌梗死後結締組織生長因子(CTGF)的調控作用以及對心肌纖維化的影響.方法 72隻C57BL雄性小鼠,隨機分為假手術組(MS組)、心肌梗死組(MI組)及藥物榦預組(MT組),每組各24隻.術後1週MT組腹腔註射環燐痠腺苷葡胺(MAC),連續給藥4週;MS組及MI組註射等量生理鹽水.各組隨機抽取10隻小鼠行心髒超聲檢測及組織病理形態學檢測;每組剩餘小鼠按照5、5、4隻隨機分成3組,分離左心室梗死區及非梗死區,分彆行羥脯氨痠含量測定,CTGF蛋白錶達採用Western blot檢測及CTGF mRNA、轉化生長因子-β1(TGF-β1) mRNA錶達採用實時定量聚閤酶鏈反應(Real-time PCR)檢測.結果 MT組射血分數(EF)為(19.83 ±3.95)％,左室短軸縮短率(FS)為(9.07±1.90)％,均高于MI組[EF:(13.65±2.14)％,FS:(6.22±1.03)％,P＜0.05];左心室每搏輸齣量(SV) MT組[(26.26±3.57)μl]優于MI組[(18.82±2.71)μl,P＜0.05].Masson染色提示MT組膠原纖維嚮非梗死區心肌浸潤減少,免疫組織化學CTGF暘性錶達較MI組減少.MT組梗死區羥脯氨痠含量[(1 388.98±86.26) μg/mg]低于MI組[(1 673.12±199.97) μg/mg,P＜0.05];非梗死區羥脯氨痠含量[(662.92 ±98.05) μg/mg]低于MI組[(855.84±83.30)μg/mg,P＜0.05].Western blot檢測結果提示MT組CTGF蛋白錶達量低于MI組(P＜0.05).Real-time PCR檢測提示MT組CTGF mRNA錶達量低于MI組(P＜0.05);TGF-β1 mRNA錶達量在梗死區及非梗死區較MI組差異無統計學意義(P＞0.05).結論 環燐痠腺苷可以抑製CTGF錶達,降低小鼠心肌梗死後膠原纖維的錶達,減輕梗死區膠原纖維嚮非梗死區心肌的浸潤程度,改善心髒功能.
목적 관찰배린산선감(cAMP)대소서심기경사후결체조직생장인자(CTGF)적조공작용이급대심기섬유화적영향.방법 72지C57BL웅성소서,수궤분위가수술조(MS조)、심기경사조(MI조)급약물간예조(MT조),매조각24지.술후1주MT조복강주사배린산선감포알(MAC),련속급약4주;MS조급MI조주사등량생리염수.각조수궤추취10지소서행심장초성검측급조직병리형태학검측;매조잉여소서안조5、5、4지수궤분성3조,분리좌심실경사구급비경사구,분별행간포안산함량측정,CTGF단백표체채용Western blot검측급CTGF mRNA、전화생장인자-β1(TGF-β1) mRNA표체채용실시정량취합매련반응(Real-time PCR)검측.결과 MT조사혈분수(EF)위(19.83 ±3.95)％,좌실단축축단솔(FS)위(9.07±1.90)％,균고우MI조[EF:(13.65±2.14)％,FS:(6.22±1.03)％,P＜0.05];좌심실매박수출량(SV) MT조[(26.26±3.57)μl]우우MI조[(18.82±2.71)μl,P＜0.05].Masson염색제시MT조효원섬유향비경사구심기침윤감소,면역조직화학CTGF양성표체교MI조감소.MT조경사구간포안산함량[(1 388.98±86.26) μg/mg]저우MI조[(1 673.12±199.97) μg/mg,P＜0.05];비경사구간포안산함량[(662.92 ±98.05) μg/mg]저우MI조[(855.84±83.30)μg/mg,P＜0.05].Western blot검측결과제시MT조CTGF단백표체량저우MI조(P＜0.05).Real-time PCR검측제시MT조CTGF mRNA표체량저우MI조(P＜0.05);TGF-β1 mRNA표체량재경사구급비경사구교MI조차이무통계학의의(P＞0.05).결론 배린산선감가이억제CTGF표체,강저소서심기경사후효원섬유적표체,감경경사구효원섬유향비경사구심기적침윤정도,개선심장공능.
Objective To observe the regulatory effects of cyclic adenosine monophosphate (cAMP) on connective tissue growth factor (CTGF) and myocardial fibrosis.Methods 72 specific pathogen free (SPF) C57BL mice were random divided into three groups:sham operation group (MS group),myocardial infarction group (MI group) and drug treatment group (MT group).MT group were injected meglumine adenosine cyclophosphate (MAC) by intraperitoneal for 4 weeks after 7 days postoperation.10 mices in each group were examined by transthoracic echocardiography and then harvested heart tissues for histopathological morphologic detection.The left mices were random divided into three groups,separated the infracted zone and non-infracted zone of left ventricle,then detected the expression level of CTGF mRNA,transforming growth factor-β1 (TGF-β1) mRNA,CTGF protein and hydroxyproline separately.Results The ejection fraction,fraction shortening and stroke volume in MT group were higher than MI group [(19.83 ±3.95)％ vs.(13.65 ±2.14)％;(9.07 ±1.90)％ vs.(6.22± 1.03) ％;(26.26 ± 3.57) μl vs.(18.82 ± 2.71) μl,P ＜ 0.05].Masson staining examination shows the degree of collagenous fiber infiltration from infracted zone to non-infracted zone in MT group was lower than MI group.Compared with MI group,the expression level of CTGF protein,CTGF mRNA and hydroxyproline in MT group decreased after intervening by MAC (P ＜ 0.05).But the TGF-β1 mRNA expression level has no statistically significant differences both in infracted zone and non-infracted zone between MT group and MI group (P ＞ 0.05).Conclusion cAMP can reduce the collagenous fiber expression level by inhibiting CTGF expression level,decrease the infiltration degree of collagenous fiber from infracted zone to non-infracted zone and improve the heart function after myocardial infarction in mice.
