中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1806-1809
,共4页
刘洋%金振晓%魏旭峰%杨剑%程亮%刘维永%俞世强
劉洋%金振曉%魏旭峰%楊劍%程亮%劉維永%俞世彊
류양%금진효%위욱봉%양검%정량%류유영%유세강
体外实验%生物相容性%便携%体外模式氧合
體外實驗%生物相容性%便攜%體外模式氧閤
체외실험%생물상용성%편휴%체외모식양합
In vitro%Biocompatibility%Wearable%Extracorporeal membrane oxygenation
目的 观察便携式人工心肺辅助装置的体外生物相容性.方法 应用肝素涂层中空纤维氧合技术与磁悬浮离心泵装置整合构建小型化、便携式心肺辅助装置.采用红细胞压积30％的牛血进行体外生物相容性检测,分别在0.5～5.0 L/min流量运转检测装置流入道及流出道血氧饱和度、氧分压、二氧化碳分压等,计算装置氧合效率.并在4.0 L/min运转24h,每4h采样检测游离血红蛋白含量,评价装置溶血性能并检测血小板激活率.转流结束后行中空氧合纤维取材苏木素-伊红(HE)染色光镜观察及4',6-二脒基-2-苯基吲哚(DAPI)染色荧光显微镜观察,评价细胞黏附情况.结果 装置流出道氧饱和度在各流量点均保持98％以上,氧合效率与转速及流量呈正相关(P＜0.01).在4.0 L/min装置运行24h,游离血红蛋白含量均在20 mg/dl以下,血小板激活均小于8％.24 h后,氧合纤维表面血细胞沉积不明显.结论 应用肝素涂层中空纤维氧合器与磁悬浮离心血泵构建便携式整合动力氧合装置,体外生物相容性可靠.
目的 觀察便攜式人工心肺輔助裝置的體外生物相容性.方法 應用肝素塗層中空纖維氧閤技術與磁懸浮離心泵裝置整閤構建小型化、便攜式心肺輔助裝置.採用紅細胞壓積30％的牛血進行體外生物相容性檢測,分彆在0.5～5.0 L/min流量運轉檢測裝置流入道及流齣道血氧飽和度、氧分壓、二氧化碳分壓等,計算裝置氧閤效率.併在4.0 L/min運轉24h,每4h採樣檢測遊離血紅蛋白含量,評價裝置溶血性能併檢測血小闆激活率.轉流結束後行中空氧閤纖維取材囌木素-伊紅(HE)染色光鏡觀察及4',6-二脒基-2-苯基吲哚(DAPI)染色熒光顯微鏡觀察,評價細胞黏附情況.結果 裝置流齣道氧飽和度在各流量點均保持98％以上,氧閤效率與轉速及流量呈正相關(P＜0.01).在4.0 L/min裝置運行24h,遊離血紅蛋白含量均在20 mg/dl以下,血小闆激活均小于8％.24 h後,氧閤纖維錶麵血細胞沉積不明顯.結論 應用肝素塗層中空纖維氧閤器與磁懸浮離心血泵構建便攜式整閤動力氧閤裝置,體外生物相容性可靠.
목적 관찰편휴식인공심폐보조장치적체외생물상용성.방법 응용간소도층중공섬유양합기술여자현부리심빙장치정합구건소형화、편휴식심폐보조장치.채용홍세포압적30％적우혈진행체외생물상용성검측,분별재0.5～5.0 L/min류량운전검측장치류입도급류출도혈양포화도、양분압、이양화탄분압등,계산장치양합효솔.병재4.0 L/min운전24h,매4h채양검측유리혈홍단백함량,평개장치용혈성능병검측혈소판격활솔.전류결속후행중공양합섬유취재소목소-이홍(HE)염색광경관찰급4',6-이미기-2-분기신타(DAPI)염색형광현미경관찰,평개세포점부정황.결과 장치류출도양포화도재각류량점균보지98％이상,양합효솔여전속급류량정정상관(P＜0.01).재4.0 L/min장치운행24h,유리혈홍단백함량균재20 mg/dl이하,혈소판격활균소우8％.24 h후,양합섬유표면혈세포침적불명현.결론 응용간소도층중공섬유양합기여자현부리심혈빙구건편휴식정합동력양합장치,체외생물상용성가고.
Objective To evaluate the biocompatibility of a wearable artificial cardiopulmonary support deviceinvitro.Methods Heparin-coating hollow fiber and axial pump were used to setup a wearable artificial cardiopulmonary support device.The In vitro biocompatibility was investigated by experimental circulate with fresh bovine blood in 30％ hematocrit.The saturation,partial oxygen pressure and partial carbon dioxidepressure of inlet and outlet were measured,and oxygen transfer rate was calculated.The plasma free hemoglobin and platelet activation were evaluated every 4 hours at 4.0 L/min for 24 hours.The hollow fiber was analyzed by hematoxylin-eosin staining (HE) and 4',6-diamidino-2-phenylindole (DAPI) staining at the end of experiment.Results The saturation of outlet was stable at over 98％ at different flow rate from 0.5-5.0 L/min.The oxygen transfer rate was compared with flow rate.The plasma free hemoglobin was lower than 20 mg/dl and the platelet activation was lower than 8％ during 24 hours.There was no significant cell consumption at the end of study.Conclusion The wearable artificial cardiopulmonary support devicewith heparin-coating hollow fiber and axial pump conducted the reliable biocompatibility in vitro.
