中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1802-1805
,共4页
杨丽华%徐玉灿%李志松%弓胜凯%张卫
楊麗華%徐玉燦%李誌鬆%弓勝凱%張衛
양려화%서옥찬%리지송%궁성개%장위
姜黄素%天冬氨酸特异性半胱氨酸蛋白酶-12%细胞凋亡%急性肺损伤%体外循环
薑黃素%天鼕氨痠特異性半胱氨痠蛋白酶-12%細胞凋亡%急性肺損傷%體外循環
강황소%천동안산특이성반광안산단백매-12%세포조망%급성폐손상%체외순배
Curcumin%Cycteinyl aspirate-specific protease 12%Apoptosis%Acute lung injury%Cardiopulmonary bypass
目的 观察姜黄素(Cur)对大鼠体外循环(CPB)所致急性肺损伤(ALI)的影响.方法 实验用SD大鼠50只,按照随机数字表法分为5组(n=10):假手术组(Sham组)、CPB组、CPB+ Cur剂量分别为100、150、200 mg/kg组(Cur-100组、Cur-150组和Cur-200组).CPB结束后留取左肺,测定肺湿/干重比(W/D)和总肺水含量(TLW),光镜下测定肺泡损伤数定量评价指标(IQA),电镜下观察肺组织超微结构改变,反转录-聚合酶链反应(RT-PCR)和Western blot分别检测天冬氨酸特异性半胱氨酸蛋白酶(Caspase)-12 mRNA及蛋白表达水平,原位末端细胞凋亡检测法(TUNEL法)检测肺组织细胞凋亡指数(AI).结果 与Sham组比较,CPB组Caspase-12 mRNA水平(0.96±0.18)及蛋白表达水平(2.79±0.74)均升高(P＜0.05),W/D(4.99±0.72)、TLW (3.99±0.72)、IQA(40.22±5.39)和AI (35.19±5.94)均增加(P＜0.05),肺组织超微结构发生明显损伤;与CPB组比较,Cur-100组、Cur-150组和Cur-200组Caspase-12 mRNA水平(0.87±0.18、0.69±0.13、0.56±0.12)及蛋白表达水平(2.88±0.79、1.96±0.58、1.34±0.49)均降低(P＜0.05),W/D (4.13±0.89、3.43±0.97、2.65±0.85)、TLW (3.13±0.89、2.43±0.97、1.65±0.85)、IQA (31.47±3.48、20.52±2.69、14.99±3.56)和AI(31.49±2.91、24.78±3.41、14.47±2.69)均下降(P＜0.05),肺组织超微结构损伤均有不同程度的减轻,以Cur-200组上述指标改善最为显著.结论 200 mg/kg的Cur对CPB引起的大鼠肺损伤具有较好的保护作用,其机制可能与其抑制Caspase-12表达,减少细胞凋亡有关.
目的 觀察薑黃素(Cur)對大鼠體外循環(CPB)所緻急性肺損傷(ALI)的影響.方法 實驗用SD大鼠50隻,按照隨機數字錶法分為5組(n=10):假手術組(Sham組)、CPB組、CPB+ Cur劑量分彆為100、150、200 mg/kg組(Cur-100組、Cur-150組和Cur-200組).CPB結束後留取左肺,測定肺濕/榦重比(W/D)和總肺水含量(TLW),光鏡下測定肺泡損傷數定量評價指標(IQA),電鏡下觀察肺組織超微結構改變,反轉錄-聚閤酶鏈反應(RT-PCR)和Western blot分彆檢測天鼕氨痠特異性半胱氨痠蛋白酶(Caspase)-12 mRNA及蛋白錶達水平,原位末耑細胞凋亡檢測法(TUNEL法)檢測肺組織細胞凋亡指數(AI).結果 與Sham組比較,CPB組Caspase-12 mRNA水平(0.96±0.18)及蛋白錶達水平(2.79±0.74)均升高(P＜0.05),W/D(4.99±0.72)、TLW (3.99±0.72)、IQA(40.22±5.39)和AI (35.19±5.94)均增加(P＜0.05),肺組織超微結構髮生明顯損傷;與CPB組比較,Cur-100組、Cur-150組和Cur-200組Caspase-12 mRNA水平(0.87±0.18、0.69±0.13、0.56±0.12)及蛋白錶達水平(2.88±0.79、1.96±0.58、1.34±0.49)均降低(P＜0.05),W/D (4.13±0.89、3.43±0.97、2.65±0.85)、TLW (3.13±0.89、2.43±0.97、1.65±0.85)、IQA (31.47±3.48、20.52±2.69、14.99±3.56)和AI(31.49±2.91、24.78±3.41、14.47±2.69)均下降(P＜0.05),肺組織超微結構損傷均有不同程度的減輕,以Cur-200組上述指標改善最為顯著.結論 200 mg/kg的Cur對CPB引起的大鼠肺損傷具有較好的保護作用,其機製可能與其抑製Caspase-12錶達,減少細胞凋亡有關.
목적 관찰강황소(Cur)대대서체외순배(CPB)소치급성폐손상(ALI)적영향.방법 실험용SD대서50지,안조수궤수자표법분위5조(n=10):가수술조(Sham조)、CPB조、CPB+ Cur제량분별위100、150、200 mg/kg조(Cur-100조、Cur-150조화Cur-200조).CPB결속후류취좌폐,측정폐습/간중비(W/D)화총폐수함량(TLW),광경하측정폐포손상수정량평개지표(IQA),전경하관찰폐조직초미결구개변,반전록-취합매련반응(RT-PCR)화Western blot분별검측천동안산특이성반광안산단백매(Caspase)-12 mRNA급단백표체수평,원위말단세포조망검측법(TUNEL법)검측폐조직세포조망지수(AI).결과 여Sham조비교,CPB조Caspase-12 mRNA수평(0.96±0.18)급단백표체수평(2.79±0.74)균승고(P＜0.05),W/D(4.99±0.72)、TLW (3.99±0.72)、IQA(40.22±5.39)화AI (35.19±5.94)균증가(P＜0.05),폐조직초미결구발생명현손상;여CPB조비교,Cur-100조、Cur-150조화Cur-200조Caspase-12 mRNA수평(0.87±0.18、0.69±0.13、0.56±0.12)급단백표체수평(2.88±0.79、1.96±0.58、1.34±0.49)균강저(P＜0.05),W/D (4.13±0.89、3.43±0.97、2.65±0.85)、TLW (3.13±0.89、2.43±0.97、1.65±0.85)、IQA (31.47±3.48、20.52±2.69、14.99±3.56)화AI(31.49±2.91、24.78±3.41、14.47±2.69)균하강(P＜0.05),폐조직초미결구손상균유불동정도적감경,이Cur-200조상술지표개선최위현저.결론 200 mg/kg적Cur대CPB인기적대서폐손상구유교호적보호작용,기궤제가능여기억제Caspase-12표체,감소세포조망유관.
Objective To investigate the effect of curcumin (Cur) on acute lung injury (ALI) induced by cardiopulmonary bypass (CPB) in rats.Methods Fifty Sprague-Dawley (SD) rats were randomly allocated into five groups (n =10):Sham operation group (Sham group),CPB group,CPB + Cur pre-treated with respectively 100,150 and 200 mg/kg groups (Cur-100 group,Cur-150 group and Cur-200 group).Rats were euthanized after CPB,and left lung tissue was excised.Wet lung weight to dry lung weight (W/D) and total lung water content (TLW) were tested.Index of quantitative evaluation for alveolar damage (IQA) was tested by light microscope.Pathological changes of ultrastructure of lung tissue were observed by transmission electron microscope.The expression levels of cycteinyl aspirate-specific protease (Caspase)-12 mRNA and protein were detected respectively by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Pneumocyte apoptosis index (AI) was determined by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method.Results Compared to Sham group,the expression levels of Caspase-12 mRNA (0.96 ± 0.18)and protein (2.79 ± 0.74) were all significantly increased (P ＜ 0.05) in CPB group,and W/D (4.99 ± 0.72),TLW (3.99 ±0.72),IQA (40.22 ±5.39),AI (35.19 ±5.94) were all notably higher (all P＜ 0.05);Ultrastructural injury in lung tissue was notably observed in CPB group.Compared to CPB group,the expression levels of Caspase-12 mRNA (0.87 ± 0.18,0.69 ± 0.13,0.56 ± 0.12) and protein (2.88 ± 0.79,1.96 ±0.58,1.34 ±0.49)were decreasingly lower (P ＜0.05) in Cur-100 group,Cur-150 group and Cur-200 group,W/D (4.13 ±0.89,3.43 ±0.97,2.65 ±0.85),TLW (3.13 ±0.89,2.43 ±0.97,1.65 ±0.85),IQA (31.47 ±3.48,20.52 ±2.69,14.99 ±3.56),AI (31.49 ±2.91,24.78 ±3.41,14.47 ± 2.69) were also decreased (all P ＜ 0.05);Ultrastructural injury in lung tissue were gradually alleviated in Cur-100 group,Cur-150 group and Cur-200 group.Conclusion Cur at a dose of 200 mg/kg has protective effects on lung against injury induced by CPB in rats,which may be related to inhibition of pneumocyte apoptosis mediated by Caspase-12.
