中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1820-1822
,共3页
曹克鑫%温丰标%吴恺%韩高扬%赵松%齐宇
曹剋鑫%溫豐標%吳愷%韓高颺%趙鬆%齊宇
조극흠%온봉표%오개%한고양%조송%제우
食管肿瘤%鳞状细胞%人类黑色素瘤相关抗原A9%预后
食管腫瘤%鱗狀細胞%人類黑色素瘤相關抗原A9%預後
식관종류%린상세포%인류흑색소류상관항원A9%예후
Esophageal neoplasms%Squamous cell%Melanoma associated antigen A9%Prognosis
目的 探讨人食管鳞状细胞癌中人类黑色素瘤相关抗原A9 (MAGE-A9)的表达及临床意义.方法 应用免疫组织化学检测53例食管鳞癌和30例正常食管组织中MAGE-A9蛋白表达水平,实时定量反转录聚合酶链反应(RT-qPCR)检测食管鳞癌癌组织和正常食管黏膜组织中MAGE-A9基因表达水平.结果 MAGE-A9在正常食管黏膜中微弱表达,而在食管鳞癌组织中显著高表达;癌组织中MAGE-A9蛋白相对表达量(3.620±2.009)明显高于正常食管黏膜组织(0.300±0.652),差异有统计学意义(P＜0.05).MAGE-A9mRNA表达水平(0.722±0.352)显著高于正常食管组织(0.223±0.292),差异有统计学意义(P＜0.05).结论 MAGE-A9在食管鳞癌中高表达,且与临床病理因素及预后相关.
目的 探討人食管鱗狀細胞癌中人類黑色素瘤相關抗原A9 (MAGE-A9)的錶達及臨床意義.方法 應用免疫組織化學檢測53例食管鱗癌和30例正常食管組織中MAGE-A9蛋白錶達水平,實時定量反轉錄聚閤酶鏈反應(RT-qPCR)檢測食管鱗癌癌組織和正常食管黏膜組織中MAGE-A9基因錶達水平.結果 MAGE-A9在正常食管黏膜中微弱錶達,而在食管鱗癌組織中顯著高錶達;癌組織中MAGE-A9蛋白相對錶達量(3.620±2.009)明顯高于正常食管黏膜組織(0.300±0.652),差異有統計學意義(P＜0.05).MAGE-A9mRNA錶達水平(0.722±0.352)顯著高于正常食管組織(0.223±0.292),差異有統計學意義(P＜0.05).結論 MAGE-A9在食管鱗癌中高錶達,且與臨床病理因素及預後相關.
목적 탐토인식관린상세포암중인류흑색소류상관항원A9 (MAGE-A9)적표체급림상의의.방법 응용면역조직화학검측53례식관린암화30례정상식관조직중MAGE-A9단백표체수평,실시정량반전록취합매련반응(RT-qPCR)검측식관린암암조직화정상식관점막조직중MAGE-A9기인표체수평.결과 MAGE-A9재정상식관점막중미약표체,이재식관린암조직중현저고표체;암조직중MAGE-A9단백상대표체량(3.620±2.009)명현고우정상식관점막조직(0.300±0.652),차이유통계학의의(P＜0.05).MAGE-A9mRNA표체수평(0.722±0.352)현저고우정상식관조직(0.223±0.292),차이유통계학의의(P＜0.05).결론 MAGE-A9재식관린암중고표체,차여림상병리인소급예후상관.
Objective To investigate the expression of melanoma associated antigen A9 (MAGE-A9) in esophageal squamous cell carcinoma (ESCC) and normal esophageal mucosa tissues,and explore its correlation with clinicopathological characteristics and prognosis.Methods The expression of MAGE-A9 protein in ESCC tissues (n =53) and normal esophageal mucosa tissues (n =30) was detected by immunohistochemistry.Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to examine the expression of MAGE-A9 mRNA in fresh ESCC specimens and normal esophageal mucosa tissues.Results The MAGE-A9 positive staining was predominantly localized in the cytoplasm of cancer cells.The expression of MAGE-A9 protein and mRNA was significantly higher in ESCC than in normal tissues (3.620 ±2.009 vs.0.300 ±0.652,P ＜0.05).Moreover,the expression level of MAGE-A9 protein in ESCC tissues was significantly correlated to smoking (P ＜ 0.05),tumor sizes (P ＜ 0.05),and lymph node metastasis (P ＜ 0.05).Cox regression analysis revealed that MAGE-A9 expression level (P ＜ 0.05) and lymph node metastasis (P ＜ 0.05) were independent prognostic factors of ESCC.Conclusion MAGE-A9 is up-regulated in ESCC.High expression of MAGE-A9 correlates with clinicopathological characteristics and unfavorable survival in ESCC.
