CAJ | 학술논문

目的比较淫羊藿素对人肝癌细胞HepG2、人肺癌细胞A549、人肾癌细胞786-O等3种人肿瘤细胞增殖的抑制效果,并探讨其作用机制.方法通过噻唑蓝(MTT)法比较淫羊藿素对肿瘤细胞HepG2、A549、786-O增殖的影响,流式细胞仪检测并确定淫羊藿素对肿瘤细胞凋亡的影响.Western blot法检测淫羊藿素处理前后细胞增殖和凋亡相关蛋白合成的变化.结果淫羊藿素均呈时间和浓度依赖性地抑制3种人肿瘤细胞的增殖,其中对786-O的抑制效果最为明显,5μmol/L淫羊藿素处理24h细胞增殖抑制率达44％,半数抑制剂量(IC50)为5.85 μmol/L;淫羊藿素促进3种肿瘤细胞凋亡,诱导786-O细胞凋亡的效果最显著,10 μmol/L淫羊藿素处理24h后,凋亡细胞比率达到45.8％;抑制周期相关蛋白细胞周期蛋白(Cyclin)A、Cyclin D1、Cyclin E、周期蛋白依赖性激酶2(CDK2)和B细胞淋巴瘤/白血病-2(bcl-2)的表达,提高促凋亡蛋白bcl-2相关X蛋白(bax)的表达.结论淫羊藿素在体外能明显抑制3种人肿瘤细胞的增殖,诱导其凋亡,对786-O效果最为显著;它可能通过调节细胞增殖和凋亡相关蛋白的表达.
목적 비교음양곽소대인간암세포HepG2、인폐암세포A549、인신암세포786-O등3충인종류세포증식적억제효과,병탐토기작용궤제.방법 통과새서람(MTT)법비교음양곽소대종류세포HepG2、A549、786-O증식적영향,류식세포의검측병학정음양곽소대종류세포조망적영향.Western blot법검측음양곽소처리전후세포증식화조망상관단백합성적변화.결과 음양곽소균정시간화농도의뢰성지억제3충인종류세포적증식,기중대786-O적억제효과최위명현,5μmol/L음양곽소처리24h세포증식억제솔체44％,반수억제제량(IC50)위5.85 μmol/L;음양곽소촉진3충종류세포조망,유도786-O세포조망적효과최현저,10 μmol/L음양곽소처리24h후,조망세포비솔체도45.8％;억제주기상관단백세포주기단백(Cyclin)A、Cyclin D1、Cyclin E、주기단백의뢰성격매2(CDK2)화B세포림파류/백혈병-2(bcl-2)적표체,제고촉조망단백bcl-2상관X단백(bax)적표체.결론 음양곽소재체외능명현억제3충인종류세포적증식,유도기조망,대786-O효과최위현저;타가능통과조절세포증식화조망상관단백적표체.
Objective To compare the effects of Icaritin on proliferation of human hepatocellular carcinoma cell line HepG2,human lung carcinoma cell line A549 and human renal cell carcinoma cell line 786-O,and study the mechanism during those processes.Methods Methyl thiazol tetrazolium (MTT) assay was used to test the effect of Icaritin on proliferation of HepG2,A549 and 786-O cells.Apoptosis of the cells was detected by flow cytometry.The expression of several key proliferative and apoptotic related proteins was investigated by Western blotting.Results Icaritin inhibited the proliferation of HepG2,A549 and 786-O cells in a time-and dose-dependent way.The inhibition effect on 786-O cells was the highest with 44％ inhibition rate after 5 μ mol/L Icaritin treatment for 24 h and 50％ inhibitory dose (IC50) was 5.85 μmol/L.Icaritin induced apoptosis in all the three cell lines with the highest effect on 786-O cells with the apoptosis rate being 45.8％.Icaritin reduced the expression of Cyclin A,Cyclin D1,Cyclin E,cyclin dependent kinase 2 (CDK2) and B cell lymphoma/leukemia-2 (bcl-2).The up-regulated protein expression of bcl-2 associated X protein (bax) was also observed.Conclusion Icaritin inhibited proliferation of HepG2,A549 and 786-O cells and induced apoptosis significantly.The effect on 786-O cells was the highest.This process may be involved in the regulation of several Cyclins,bcl-2-associated X protein (bax) and bcl-2.