CAJ | 학술논문

目的观察核转录因子-κB(NF-κB)信号通路对甲状腺乳头状癌(PTC)细胞株CGTHW3生物学行为的影响.方法培养PTC细胞株CGTH W3,将细胞随机分为3组:NF-κB信号通路激活剂组、NF-κB信号通路抑制剂组及空白对照组.用细胞计数试剂盒(CCK-8)法观察细胞增殖情况,流式细胞仪分析细胞凋亡率及周期分布,Transwell小室实验评价迁移能力.结果 (1)NF-κB信号通路的激活可显著促进CGTH W3细胞的增殖;(2)与NF-κB信号通路抑制剂组[(59.17±2.32)％]和空白对照组[(28.50±1.71)％]比较,NF-κB信号通路激活剂组的细胞凋亡比例[(19.85±0.93)％]明显下降(P＜0.05);(3)与NF-κB信号通路抑制剂组和空白对照组比较,NF-κB信号通路激活剂组G1期细胞显著降低,而S期细胞显著升高(P＜0.05);(4) NF-κB信号通路激活组细胞迁移数为(157±8)个,与NF-κB信号通路抑制剂组[(30±2)个]和空白对照组[(52±4)个]比较,细胞迁移能力明显增强(P＜0.05).结论 NF-κB在PTC细胞增殖、凋亡及迁移等过程中起重要作用.
목적 관찰핵전록인자-κB(NF-κB)신호통로대갑상선유두상암(PTC)세포주CGTHW3생물학행위적영향.방법 배양PTC세포주CGTH W3,장세포수궤분위3조:NF-κB신호통로격활제조、NF-κB신호통로억제제조급공백대조조.용세포계수시제합(CCK-8)법관찰세포증식정황,류식세포의분석세포조망솔급주기분포,Transwell소실실험평개천이능력.결과 (1)NF-κB신호통로적격활가현저촉진CGTH W3세포적증식;(2)여NF-κB신호통로억제제조[(59.17±2.32)％]화공백대조조[(28.50±1.71)％]비교,NF-κB신호통로격활제조적세포조망비례[(19.85±0.93)％]명현하강(P＜0.05);(3)여NF-κB신호통로억제제조화공백대조조비교,NF-κB신호통로격활제조G1기세포현저강저,이S기세포현저승고(P＜0.05);(4) NF-κB신호통로격활조세포천이수위(157±8)개,여NF-κB신호통로억제제조[(30±2)개]화공백대조조[(52±4)개]비교,세포천이능력명현증강(P＜0.05).결론 NF-κB재PTC세포증식、조망급천이등과정중기중요작용.
Objective To observe the effects of nuclear factor-κB (NF-κB) signaling on the biological behavior of papillary thyroid carcinoma cell line CGTH W3.Methods Cultured CGTH W3 cells were randomly divided into 3 groups:NF-κB signaling activator lipopolysaccharide (LPS),NF-κB signaling inhibitor PDTC and phosphate buffer (PBS).Cell proliferation was observed with cell counting kit-8 (CCK-8) method.Cell apoptosis rate and cell cycle distribution were analyzed by flow cytometry.The migration ability was tested by transwell methods.Results (1) Activation of NF-κB signaling could promote the proliferation of CGTH W3 cell line.(2) Compared with group of NF-κB signaling inhibitor (59.17 ± 2.32) ％ and blank control group (28.50 ± 1.71) ％,the cell apoptosis ration of NF-κB signaling activator group (19.85 ± 0.93) ％ decreased significantly (P ＜ 0.05).(3) Compared with group of NF-κB signaling inhibitor and blank control group,the G1 phase was dramatically reduced and the S phase was significant increased (P ＜ 0.05).(4) Compared with group of NF-κB signaling inhibitor (30 ± 2) and blank control group (52 ± 4),the cell migration number of NF-κB signaling activator group was 157 ± 8 (P ＜ 0.05).Conclusion NF-κB signaling pathway plays an important role in the promotion of cell proliferation and migration ability,inhibition of cell apoptosis of PTC cell line CGTH W3,which establishs the NF-κB signaling pathway as a target for tumor therapy.