中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1915-1918
,共4页
刘霞%于泳%卢高峰%黄煌%刘超%郑鹏远%韩娜
劉霞%于泳%盧高峰%黃煌%劉超%鄭鵬遠%韓娜
류하%우영%로고봉%황황%류초%정붕원%한나
慢性心理应激%肠易激综合征%远端结肠%平滑肌%收缩
慢性心理應激%腸易激綜閤徵%遠耑結腸%平滑肌%收縮
만성심리응격%장역격종합정%원단결장%평활기%수축
Chronic psychological stress%Irritable bowel syndrome%Distal colon%Smooth muscles%Contraction
目的 观察慢性心理应激对大鼠远端结肠收缩的影响.方法 将SD大鼠随机分为对照组和实验组,束缚制动法制备慢性心理应激大鼠模型,离体灌流法观察远端结肠平滑肌的自发收缩活动及乙酰胆碱(Ach)对平滑肌收缩的影响.结果 行为学测定结果表明成功制备慢性心理应激大鼠模型.对照组大鼠纵形肌和环形肌的自发收缩幅度均显著低于实验组[(0.42 ±0.09)g比(0.58 ±0.10)g和(0.52±0.11)g比(0.67±0.11)g,P＜0.05].Ach剂量依赖性增加了各组大鼠远端结肠平滑肌的自发收缩幅度(P＜0.01),其在实验组的效应显著高于对照组(P＜0.05).结论 在慢性心理应激大鼠模型,远端结肠平滑肌的收缩强度及其对Ach的反应均增加.
目的 觀察慢性心理應激對大鼠遠耑結腸收縮的影響.方法 將SD大鼠隨機分為對照組和實驗組,束縳製動法製備慢性心理應激大鼠模型,離體灌流法觀察遠耑結腸平滑肌的自髮收縮活動及乙酰膽堿(Ach)對平滑肌收縮的影響.結果 行為學測定結果錶明成功製備慢性心理應激大鼠模型.對照組大鼠縱形肌和環形肌的自髮收縮幅度均顯著低于實驗組[(0.42 ±0.09)g比(0.58 ±0.10)g和(0.52±0.11)g比(0.67±0.11)g,P＜0.05].Ach劑量依賴性增加瞭各組大鼠遠耑結腸平滑肌的自髮收縮幅度(P＜0.01),其在實驗組的效應顯著高于對照組(P＜0.05).結論 在慢性心理應激大鼠模型,遠耑結腸平滑肌的收縮彊度及其對Ach的反應均增加.
목적 관찰만성심리응격대대서원단결장수축적영향.방법 장SD대서수궤분위대조조화실험조,속박제동법제비만성심리응격대서모형,리체관류법관찰원단결장평활기적자발수축활동급을선담감(Ach)대평활기수축적영향.결과 행위학측정결과표명성공제비만성심리응격대서모형.대조조대서종형기화배형기적자발수축폭도균현저저우실험조[(0.42 ±0.09)g비(0.58 ±0.10)g화(0.52±0.11)g비(0.67±0.11)g,P＜0.05].Ach제량의뢰성증가료각조대서원단결장평활기적자발수축폭도(P＜0.01),기재실험조적효응현저고우대조조(P＜0.05).결론 재만성심리응격대서모형,원단결장평활기적수축강도급기대Ach적반응균증가.
Objective To investigate the effect of chronic psychological stress on the motility of distal colon.Methods SD rats were divided into control group and experimental group randomly.Chronic psychological stress rat model was induced by bondage immobilization.Feces granules counting,tail-suspension test and sucrose consumption test were used for behavior test.In vitro perfusion method was employed to investigate the motility of smooth muscles in distal colon and their response to acetylcholine (Ach).Results Chronic psychological stress rat model was established successfully.The amplitude but not the frequency of spontaneous contractions of longitudinal and circular muscles in experimental group increased significantly compared with that of control group [(0.42 ± 0.09) g vs.(0.58 ± 0.10) g and (0.52 ± 0.11) g vs.(0.67 ± 0.11) g respectively,P ＜ 0.05].Ach dose-dependently elevated the amplitude but not the frequency of spontaneous contractions of smooth muscles in both groups.Additionally,the response of smooth muscles to Ach was significantly higher in experimental group than that of control group (P ＜ 0.05).Conclusion Chronic psychological stress increases the motility of smooth muscles in distal colon and elevates their response to Ach,which is a possible mechanism responsible for diarrhea of irritable bowel syndrome (IBS).
