中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
8期
1943-1946
,共4页
龙湟哲%姜华茂%傅德望%袁观远%贾凡振%左知润
龍湟哲%薑華茂%傅德望%袁觀遠%賈凡振%左知潤
룡황철%강화무%부덕망%원관원%가범진%좌지윤
钩藤碱%骶上脊髓损伤%T型钙通道
鉤籐堿%骶上脊髓損傷%T型鈣通道
구등감%저상척수손상%T형개통도
Rhynchophylline%Suprasacral spinal cord injury%T type calcium channel
目的 探讨钩藤碱对骶上脊髓损伤(SCI)大鼠的膀胱功能障碍治疗作用与膀胱逼尿肌T型钙通道受体亚型(α1G)表达改变的关系.方法 将60只SD大鼠随机分为钩藤碱组、对照组、假手术组,每组20只,造模后分别腹腔注射钩藤碱5 ml/(kg·d)、等体积生理盐水、不给予药物干预.用尿动力仪评估膀胱功能;膀胱组织苏木素-伊红(HE)染色;用免疫组织化学法检测膀胱酪氨酸激酶受体(C-kit)、α1G的表达.结果 钩藤碱组膀胱最大容量较对照组增加(64.00±0.42)％、漏尿点压及收缩频率分别下降(16.00±0.31)％和(13.00±0.26)％ (P ＜0.01).HE染色显示钩藤碱组逼尿肌紊乱及水肿程度较对照组低.免疫组织化学检测显示钩藤碱组大鼠C-kit、α1G受体表达量较对照组明显下降.结论 钩藤碱可抑制膀胱Cajal间质细胞(ICCs)表面α1G的表达,从而降低骶上脊髓损伤后大鼠膀胱逼尿肌兴奋性.
目的 探討鉤籐堿對骶上脊髓損傷(SCI)大鼠的膀胱功能障礙治療作用與膀胱逼尿肌T型鈣通道受體亞型(α1G)錶達改變的關繫.方法 將60隻SD大鼠隨機分為鉤籐堿組、對照組、假手術組,每組20隻,造模後分彆腹腔註射鉤籐堿5 ml/(kg·d)、等體積生理鹽水、不給予藥物榦預.用尿動力儀評估膀胱功能;膀胱組織囌木素-伊紅(HE)染色;用免疫組織化學法檢測膀胱酪氨痠激酶受體(C-kit)、α1G的錶達.結果 鉤籐堿組膀胱最大容量較對照組增加(64.00±0.42)％、漏尿點壓及收縮頻率分彆下降(16.00±0.31)％和(13.00±0.26)％ (P ＜0.01).HE染色顯示鉤籐堿組逼尿肌紊亂及水腫程度較對照組低.免疫組織化學檢測顯示鉤籐堿組大鼠C-kit、α1G受體錶達量較對照組明顯下降.結論 鉤籐堿可抑製膀胱Cajal間質細胞(ICCs)錶麵α1G的錶達,從而降低骶上脊髓損傷後大鼠膀胱逼尿肌興奮性.
목적 탐토구등감대저상척수손상(SCI)대서적방광공능장애치료작용여방광핍뇨기T형개통도수체아형(α1G)표체개변적관계.방법 장60지SD대서수궤분위구등감조、대조조、가수술조,매조20지,조모후분별복강주사구등감5 ml/(kg·d)、등체적생리염수、불급여약물간예.용뇨동력의평고방광공능;방광조직소목소-이홍(HE)염색;용면역조직화학법검측방광락안산격매수체(C-kit)、α1G적표체.결과 구등감조방광최대용량교대조조증가(64.00±0.42)％、루뇨점압급수축빈솔분별하강(16.00±0.31)％화(13.00±0.26)％ (P ＜0.01).HE염색현시구등감조핍뇨기문란급수종정도교대조조저.면역조직화학검측현시구등감조대서C-kit、α1G수체표체량교대조조명현하강.결론 구등감가억제방광Cajal간질세포(ICCs)표면α1G적표체,종이강저저상척수손상후대서방광핍뇨기흥강성.
Objective To investigate the relationship between the efficacy of rhynchophylline for bladder dysfunction in suprasacral spinal cord injury rats and T type calcium channel changes in bladder detusor.Methods Sixty female Sprague Dawley rats were randomly allocated into rhynchophylline group,control group,and sham operation group (n =20 each).After models established,rhynchophylline group was given intraperitoneal injection of rhynchophylline [5 ml/(kg·d)] and control group given the same volume of saline,and sham group given no treatment.Urodynamics was monitored to evaluate the founction of bladder.Bladder tissue was stained with hematoxylin and eosin (HE) method.Immunohistochemical method was used to detect the expression level of α1 G and C-kit.Results The maximum bladder capacity in rhynchophylline group was increased by (64.00 ± 0.42) ％ compared to control group,while the bladder leak point pressure and contraction frequency in rhynchophylline group were declined by (16.00 ± 0.31) ％ and (13.00 ± 0.26) ％,respectively (P ＜ 0.01).HE staining showed detrusor disorder and mucosa edema in control group were more obvious than rhynchophylline group.The α1G and C-kit expression was significantly reduced in rhynchophylline group as compared with control group.Conclusion Rhynchophylline restrained the expression of T type calcium channel subtype α1 G on the surface of interstitial cells of Cajal (ICCs) in rat bladder,and then reduced the excitability of detrusor caused by suprasacral spinal cord injury.
