CAJ | 학술논문

目的观察糖皮质激素诱导的亮氨酸拉链(GILZ)对脊髓损伤的保护作用.方法 T淋巴细胞GILZ转基因小鼠及野生型小鼠(WT)制备脊髓损伤模型,观察GILZ对脊髓损伤的保护作用以及机制.结果 GILZ小鼠在脊髓损伤后的组织学评分为1.1分,显著低于WT模型小鼠的4.2分(P＜0.05).WT小鼠的胶质纤维酸性蛋白(GFAP)表达、促炎因子表达、CD4和CD8型T细胞表达和细胞凋亡在脊髓损伤后均显著增强(P＜0.05).而GILZ模型小鼠均可降低上述反应(P＜0.05).GILZ通过抑制核因子(NF)-κB的核转移抑制炎性反应,抑制凋亡基因和增强抗凋亡基因减少细胞凋亡.结论 T淋巴细胞高表达糖皮质激素可以对小鼠脊髓损伤产生保护作用.
목적 관찰당피질격소유도적량안산랍련(GILZ)대척수손상적보호작용.방법 T림파세포GILZ전기인소서급야생형소서(WT)제비척수손상모형,관찰GILZ대척수손상적보호작용이급궤제.결과 GILZ소서재척수손상후적조직학평분위1.1분,현저저우WT모형소서적4.2분(P＜0.05).WT소서적효질섬유산성단백(GFAP)표체、촉염인자표체、CD4화CD8형T세포표체화세포조망재척수손상후균현저증강(P＜0.05).이GILZ모형소서균가강저상술반응(P＜0.05).GILZ통과억제핵인자(NF)-κB적핵전이억제염성반응,억제조망기인화증강항조망기인감소세포조망.결론 T림파세포고표체당피질격소가이대소서척수손상산생보호작용.
Objective To determine the protective effects of glucocorticoid-induced leucine zipper (GILZ) on spinal cord injury in mice.Methods T lymphocyte GILZ transgenic mice and wild-type mice (WT) underwent spinal cord injury model,and the protective effect and mechanism of GILZ on spinal cord injury were also observed.Results The histological score in GILZ mice undergoing spinal cord injury was 1.1,which was significantly lower than that of WT model mice of 4.2 (P ＜ 0.05).The expression of glial fibrillary acidic protein (GFAP),pro-inflammatory factor,CD4 and CD8 T cells,as well as apoptosis in WT mice were significantly enhanced after spinal cord injury.Reduction of above reaction in GILZ model mice was all observed (P ＜ 0.05).The anti-inflammation or anti-apoptosis of GILZ was achieved through prevention of nuclear factor-κB (NF-κB) translocation and elevation of anti-apoptotic gene expression (P ＜ 0.05).Conclusion T lymphocyte highly expressed glucocorticoid can protect the spinal cord injury in mice.GILZ can be used as the target for the treatment of spinal cord injury.