CAJ | 학술논문

背景:将羟基磷灰石与聚乙烯醇水凝胶复合之后应用于软骨缺损修复中,可在软骨连接部位产生良好的生物活性,有效促进骨细胞的生长,提高植入材料的稳定性和生物活性.目的:观察聚乙烯醇/羟基磷灰石复合水凝胶修复兔膝关节软骨缺损的组织相容性.方法:取 20 只新西兰大白兔,随机分为空白对照组(n=6)与实验组(n=14),构建单侧膝关节软骨缺损模型,空白对照组不予以修复,实验组予以聚乙烯醇/羟基磷灰石复合水凝胶修复.术后 4,8,12 周获取膝关节标本进行大体观察及组织学观察.结果与结论:空白对照组关节软骨面在术后12周内始终未得到修复,软骨下缺损存在肉芽组织充填现象,组织学也未见明显修复.实验组术后4周可见聚乙烯醇/羟基磷灰石复合水凝胶填充于缺损处,与周围软骨组织之间连接紧密,且存在清晰的界限,未出现细胞长入情况;至 12 周时,聚乙烯醇/羟基磷灰石复合水凝胶呈白色、半透明状,表面平坦,与周围软骨组织之间存在清晰界限,两者交界面存在软骨细胞大量增殖现象,与周围组织发生紧密结合,二者之间无间隙,底部与软骨下骨紧密连接,并存在骨样组织长入.表明聚乙烯醇羟基磷灰石复合水凝胶修复兔膝关节软骨缺损具有良好的组织相容性.
배경:장간기린회석여취을희순수응효복합지후응용우연골결손수복중,가재연골련접부위산생량호적생물활성,유효촉진골세포적생장,제고식입재료적은정성화생물활성.목적:관찰취을희순/간기린회석복합수응효수복토슬관절연골결손적조직상용성.방법:취 20 지신서란대백토,수궤분위공백대조조(n=6)여실험조(n=14),구건단측슬관절연골결손모형,공백대조조불여이수복,실험조여이취을희순/간기린회석복합수응효수복.술후 4,8,12 주획취슬관절표본진행대체관찰급조직학관찰.결과여결론:공백대조조관절연골면재술후12주내시종미득도수복,연골하결손존재육아조직충전현상,조직학야미견명현수복.실험조술후4주가견취을희순/간기린회석복합수응효전충우결손처,여주위연골조직지간련접긴밀,차존재청석적계한,미출현세포장입정황;지 12 주시,취을희순/간기린회석복합수응효정백색、반투명상,표면평탄,여주위연골조직지간존재청석계한,량자교계면존재연골세포대량증식현상,여주위조직발생긴밀결합,이자지간무간극,저부여연골하골긴밀련접,병존재골양조직장입.표명취을희순간기린회석복합수응효수복토슬관절연골결손구유량호적조직상용성.
BACKGROUND:Hydroxyapatite and polyvinyl alcohol composite hydrogel used for the repair of cartilage defects can have good biological activity in the cartilage joints, effectively promote the growth of bone cels, and enhance the stability and biological activity of implant materials. OBJECTIVE:To explore the histocompatibility of polyvinyl alcohol/hydroxyapatite composite hydrogel transplantation for the repair of articular cartilage defects. METHODS: Twenty New Zealand rabbits were enroled and randomly divided into control group (n=6) and experimental group (n=14). Unilateral knee cartilage defect models were constructed in the two groups. No treatment was done in the control group, and polyvinyl alcohol/hydroxyapatite composite hydrogel transplantation was performed in the experimental group. After 4, 8, 12 weeks, the animals were kiled to colect knee joint specimens for gross observation and histological observation. RESULTS AND CONCLUSION:The surface of articular cartilage in the control group was not recovered at 12 weeks after operation, and granulation tissues were ful of cartilage defects. In the experimental group, the polyvinyl alcohol/hydroxyapatite composite hydrogel was filed in the defects, and tightly connected with the surrounding cartilage tissues with clear boundary and no cel ingrowth at 4 weeks after operation. Until the 12th week, in the experimental group, the composite hydrogel was white and translucent with smooth surface. There was a clear boundary between the composite hydrogel and surrounding cartilage tissues, a great amount of cartilage cels proliferated in the interface that tightly bound to the surrounding tissues, and osteoid tissue grew. These findings indicate that the polyvinyl alcohol/hydroxyapatite composite hydrogel has good histocompatibility in the repair of cartilage defects of rabbit knee joints.